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show all sequences of 1.8.5.5

Engineering hydrogen sulfide production and cadmium removal by expression of the thiosulfate reductase gene (phsABC) from Salmonella enterica serovar typhimurium in Escherichia coli

Bang, S.W.; Clark, D.S.; Keasling, J.D.; Appl. Environ. Microbiol. 66, 3939-3944 (2000)

Data extracted from this reference:

Application
Application
Commentary
Organism
environmental protection
Escherichia coli strains harboring thiosulfate reductase gene phsABC expression constructs show higher thiosulfate reductase activity and produce significantly more sulfide than the control strains under both aerobic and anaerobic conditions. The most effecitve expression construct produces thiosulfate reductase at the highest level and removes the most cadmium from solution under anaerobic conditions: 98% of all concentrations up to 150 microM and 91% of 200 microM. The metal removed from solution precipitates as a complex of cadmium and sulfur, most likely cadmium sulfide
Salmonella enterica subsp. enterica serovar Typhimurium
Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Salmonella enterica subsp. enterica serovar Typhimurium
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
P37600 and P0A1I1
P37600 i.e. subunit PhsA, P0A1I1 i.e. subunit PhsB
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Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720
P37600 and P0A1I1
P37600 i.e. subunit PhsA, P0A1I1 i.e. subunit PhsB
-
Application (protein specific)
Application
Commentary
Organism
environmental protection
Escherichia coli strains harboring thiosulfate reductase gene phsABC expression constructs show higher thiosulfate reductase activity and produce significantly more sulfide than the control strains under both aerobic and anaerobic conditions. The most effecitve expression construct produces thiosulfate reductase at the highest level and removes the most cadmium from solution under anaerobic conditions: 98% of all concentrations up to 150 microM and 91% of 200 microM. The metal removed from solution precipitates as a complex of cadmium and sulfur, most likely cadmium sulfide
Salmonella enterica subsp. enterica serovar Typhimurium
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Salmonella enterica subsp. enterica serovar Typhimurium
Other publictions for EC 1.8.5.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725273
Stoffels
Thiosulfate reduction in Salmo ...
Salmonella enterica
J. Bacteriol.
194
475-485
2012
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645578
Bang
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Cadmium, lead, and zinc remova ...
Salmonella enterica subsp. enterica serovar Typhimurium
Biotechnol. Lett.
22
1331-1335
2000
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737488
Bang
Engineering hydrogen sulfide p ...
Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720
Appl. Environ. Microbiol.
66
3939-3944
2000
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738317
Alami
Cloning and characterization o ...
Salmonella enterica subsp. enterica serovar Typhimurium
Gene
156
53-57
1995
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1
1
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738502
Heinzinger
Sequence analysis of the phs o ...
Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720
J. Bacteriol.
177
2813-2820
1995
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3
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6
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738491
Clark
The phs gene and hydrogen sulf ...
Salmonella enterica subsp. enterica serovar Typhimurium
J. Bacteriol.
169
2391-2397
1987
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