Cloned (Comment) | Organism |
---|---|
gene ht2, quantitative RT-PCR enzyme expression analysis | Schizosaccharomyces pombe |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction and analysis of an enzyme knockout strain DELTAsqr, phenotypes, overview | Schizosaccharomyces pombe |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Schizosaccharomyces pombe | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
n H2S + n quinone | Schizosaccharomyces pombe | - |
polysulfide + n quinol | - |
? | |
n H2S + n quinone | Schizosaccharomyces pombe ATCC 24843 | - |
polysulfide + n quinol | - |
? | |
n H2S + n quinone | Schizosaccharomyces pombe 972 | - |
polysulfide + n quinol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Schizosaccharomyces pombe | O94284 | - |
- |
Schizosaccharomyces pombe 972 | O94284 | - |
- |
Schizosaccharomyces pombe ATCC 24843 | O94284 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
n H2S + n quinone | - |
Schizosaccharomyces pombe | polysulfide + n quinol | - |
? | |
n H2S + n quinone | - |
Schizosaccharomyces pombe ATCC 24843 | polysulfide + n quinol | - |
? | |
n H2S + n quinone | - |
Schizosaccharomyces pombe 972 | polysulfide + n quinol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HMT2 | - |
Schizosaccharomyces pombe |
SQR | - |
Schizosaccharomyces pombe |
sulfide-quinone oxidoreductase | - |
Schizosaccharomyces pombe |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Schizosaccharomyces pombe |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Schizosaccharomyces pombe |
General Information | Comment | Organism |
---|---|---|
malfunction | enzyme Sqr knockout leads to morphological changes and functional deficiencies of mitochondria and apoptosis in Schizosaccharomyces pombe. The Sqr knockout strain displays the same phenotypes as the cysteine-synthesis-deficient strain. Cysteine addition complements the effects caused by Sqr knockout. Sqr knockout also results in physiological changes. The DELTAsqr strain shows reduced cell viability compared to the wild-type strain when cultivated in YES medium. There are more cells of early apoptosis in DELTAsqr culture than that in wild-type culture. Sqr knockout impaires mitochondrial health. Phenotypes and transcription and metabolism changes caused by sqr knockout, overview | Schizosaccharomyces pombe |
metabolism | in Schizosaccharomyces pombe, Sqr is the main reactive sulfur species (RSS) producer in mitochondria, and RSS instead of H2S is used by cysteine synthase to synthesize cysteine. In terms of RSS metabolism, Schizosaccharomyces pombe does not contain genes encoding cystathionine beta-synthase (Cbs), cystathionine gamma-lyase (Cse), cysteinyl-tRNA synthetase 2 (Crs2), or persulfide dioxygenase (Pdo) | Schizosaccharomyces pombe |
additional information | reactive sulfur species (RSS) include inorganic polysulfide (HSnH, n>2), organic polysulfide (RSnH, n>2), and polysulfane (RSnR, n>2) | Schizosaccharomyces pombe |
physiological function | sulfide:quinone oxidoreductase (Sqr), which oxidizes hydrogen sulfide to reactive sulfur species (RSS), is indispensable to mitochondria health in the eukaryotic model microorganism Schizosaccharomyces pombe. RSS play critical functions in the cell, such as signaling, redox homeostasis maintenance, and metabolic regulation | Schizosaccharomyces pombe |