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Literature summary for 1.8.5.4 extracted from

  • Mannacharaju, M.; Chittybabu, S.; Sheikh John, S.; Somasundaram, S.; Ganesan, S.
    Bio catalytic oxidation of sulphide laden wastewater from leather industry using sulfide Quinone oxidoreductase immobilized bio reactor (2020), Biocatal. Biotransform., 38, 123-137 .
No PubMed abstract available
Search for more literature for 1.8.5.4

Application

Application Comment Organism
environmental protection anaerobic treatment of sulphate rich wastewater results in high amount of sulfide in liquid phase and gaseous phase. Sulfide is malodorous in gaseous phase and toxic even at very low concentrations in liquid phase and causes objectionable environmental issues. Sulfide present in the up-flow anaerobic sludge blanket (UASB)-treated post tanning wastewater is oxidized into elemental sulfur using sulfide:quinone oxidoreductase (SQR) immobilized on functionalized carbon-silica matrix (FCSM) in a packed bed reactor. Optimum conditions for immobilization of SQR onto FCSM are pH, 7.0, 40°C, and 10mg/g SQR during 240 min. The immobilization of SQR onto FCSM obeys the Langmuir isotherm model. The maximum sulfide oxidation is 99% at HRT of 15 h with residual sulfide of 2.4 mg/l. The formation of elemental Sulphur is confirmed by XRD studies Alkalihalobacillus clausii

Protein Variants

Protein Variants Comment Organism
additional information sulfide present in the up-flow anaerobic sludge blanket (UASB)-treated post tanning wastewater is oxidized into elemental sulfur using sulfide:quinone oxidoreductase (SQR) immobilized on functionalized carbon-silica matrix (FCSM) in a packed bed reactor. Optimum conditions for immobilization of SQR onto FCSM are pH, 7.0, 40°C, and 10mg/g SQR during 240 min. The immobilization of SQR onto FCSM obeys the Langmuir isotherm model. The maximum sulfide oxidation is 99% at HRT of 15 h with residual sulfide of 2.4 mg/l. The formation of elemental Sulphur is confirmed by XRD studies. Enzyme immobilization method evaluation and optimization, adsorption kinetics and thermodynamics, detailed overview Alkalihalobacillus clausii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
n H2S + n quinone Alkalihalobacillus clausii
-
 polysulfide + n quinol
-
 ?

Organism

Organism UniProt Comment Textmining
Alkalihalobacillus clausii
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.1
-
 purified enzyme, substrate decylubiquinone, pH 7.0, 40°C Alkalihalobacillus clausii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
n H2S + n quinone
-
 Alkalihalobacillus clausii polysulfide + n quinol
-
 ?
n Na2S + n decylubiquinone
-
 Alkalihalobacillus clausii polysulfide + n decylubiquinol + 2 Na+
-
 ?

Synonyms

Synonyms Comment Organism
SQR
-
 Alkalihalobacillus clausii
sulfide: quinone oxidoreductase
-
 Alkalihalobacillus clausii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Alkalihalobacillus clausii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 60 SQR activity is gradually decreased at temperature below 30°C and above 40°C Alkalihalobacillus clausii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 stable at Alkalihalobacillus clausii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 8
-
 Alkalihalobacillus clausii

pH Range

pH Minimum pH Maximum Comment Organism
2 10 activity range, SQR activity decreases rapidly at the pH above 8.0 and also below pH 5.0. There is no SQR activity found at pH above 10.0 and at pH below 2.0 Alkalihalobacillus clausii

Cofactor

Cofactor Comment Organism Structure
decylubiquinone
-
 Alkalihalobacillus clausii
quinone
-
 Alkalihalobacillus clausii