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Literature summary for 1.8.5.4 extracted from
- Cytoplasmic localization of sulfide quinone oxidoreductase and persulfide dioxygenase of Cupriavidus pinatubonensis JMP134 (2017), Appl. Environ. Microbiol., 83, e01820-17 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Rhodanese | speeds up the reaction of polysulfide with glutathione (GSH) to produce glutathione persulfide (GSSH) | Cupriavidus pinatubonensis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene sqr, recombinant expression of GFP-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Cupriavidus pinatubonensis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | for analysis of the membrane association of CpSQR: construction of C-terminally truncated CpSQR mutants containing the N-terminal fragment sx, where x stands for the number of amino acid residues from the N terminus (x = 26, 49, 71, 83, 107, 115, 128, 157, 176, 230, 359, or 450). Generation of a CpSQR-PhoA fusion protein and comparison with the TolB-PhoA fusion protein. Cells with the fusion of CpSQR and PhoA show SQR activity, while other fusions with SQR fragments have no SQR activity, full-length PhoA and TolB-PhoA are used as the positive controls | Cupriavidus pinatubonensis |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cell membrane | CpSQR is located on the cytoplasmic side of the membrane | Cupriavidus pinatubonensis | - |
- |
| additional information | localization of SQR and PDO inside the cytoplasm in the vicinity of the membrane | Cupriavidus pinatubonensis | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| n H2S + n ubiquinone | Cupriavidus pinatubonensis | - |
polysulfide + n ubiquinol | - |
? |  |
| n Na2S + n ubiquinone | Cupriavidus pinatubonensis | - |
polysulfide + n ubiquinol | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cupriavidus pinatubonensis | Q46V88 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| n H2S + n ubiquinone | - |
Cupriavidus pinatubonensis | polysulfide + n ubiquinol | - |
? | |
| n Na2S + n ubiquinone | - |
Cupriavidus pinatubonensis | polysulfide + n ubiquinol | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CpSQR | - |
Cupriavidus pinatubonensis |
| SQR | - |
Cupriavidus pinatubonensis |
| sulfide:quinone oxidoreductase | - |
Cupriavidus pinatubonensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Cupriavidus pinatubonensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Cupriavidus pinatubonensis |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | in bacteria, sulfide:quinone oxidoreductase (SQR) oxidizes sulfide to polysulfide, rhodanese speeds up the reaction of polysulfide with glutathione (GSH) to produce glutathione persulfide (GSSH), and persulfide dioxygenase (PDO) oxidizes GSSH to sulfite, which spontaneously reacts with polysulfide to produce thiosulfate. Ubiquinone is a cosubstrate in the reaction | Cupriavidus pinatubonensis |
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