Cloned (Comment) | Organism |
---|---|
- |
Acidithiobacillus ferrooxidans |
Crystallization (Comment) | Organism |
---|---|
native or in complex with decylubiquinone, hanging drop vapor diffusion method, using 30% (w/v) polyethylene glycol 600, 0.1 M 2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)propane-1,3-diol (pH 5.5 or pH 6.5), 0.1 M magnesium sulfate, and 0.05% (w/v) n-dodecyl beta-D-maltoside | Acidithiobacillus ferrooxidans |
Protein Variants | Comment | Organism |
---|---|---|
C128A | in the decylubiquinone assay, the mutant shows 30-35% activity compared to the wild type enzyme. However, in the FAD reduction assay, both the wild type and the Cys128Ala variant are fully active (100%) | Acidithiobacillus ferrooxidans |
C160A | inactive | Acidithiobacillus ferrooxidans |
C356A | inactive | Acidithiobacillus ferrooxidans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0028 | - |
Sulfide | in 50 mM 2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)propane-1,3-diol (pH 6.5), temperature not specified in the publication | Acidithiobacillus ferrooxidans | |
0.022 | - |
decylubiquinone | in 50 mM 2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)propane-1,3-diol (pH 6.5), temperature not specified in the publication | Acidithiobacillus ferrooxidans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Acidithiobacillus ferrooxidans | 16020 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acidithiobacillus ferrooxidans | B7JBP8 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Acidithiobacillus ferrooxidans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzymatic reaction catalyzed by sulfide:quinone oxidoreductase includes the oxidation of sulfide compounds H2S, HS-, and S2- to soluble polysulfide chains or to elemental sulfur in the form of octasulfur rings | Acidithiobacillus ferrooxidans | ? | - |
? | |
sulfide + decylubiquinone | - |
Acidithiobacillus ferrooxidans | sulfur + decylubiquinol | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | x-ray crystallography | Acidithiobacillus ferrooxidans |
Synonyms | Comment | Organism |
---|---|---|
sulfide:quinone oxidoreductase | - |
Acidithiobacillus ferrooxidans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | noncovalently bound | Acidithiobacillus ferrooxidans |