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Literature summary for 1.8.5.3 extracted from
- Modulation of the substrate specificity of Escherichia coli dimethylsulfoxide reductase (1998), Eur. J. Biochem., 251, 510-515.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A178Q | mutation in subunit DmsA. About 1200% of wild-type catalytic efficiency | Escherichia coli |
| A178Q | mutation in subunit DmsA. Mutant is functionally impairment, with abnormal anaerobic growth with dimethylsulfoxide as the sole terminal acceptor, in a recombinant strain deleted for chromosomal dmsABC | Escherichia coli |
| A181T | mutation in subunit DmsA. About 300% of wild-type catalytic efficiency | Escherichia coli |
| G167N | mutation in subunit DmsA. About 20% of wild-type catalytic efficiency | Escherichia coli |
| G167N | mutation in subunit DmsA. Mutant is functionally impairment, with abnormal anaerobic growth with dimethylsulfoxide as the sole terminal acceptor, in a recombinant strain deleted for chromosomal dmsABC | Escherichia coli |
| G190D | mutation in subunit DmsA. About 80% of wild-type catalytic efficiency | Escherichia coli |
| G190V | mutation in subunit DmsA. About 180% of wild-type catalytic efficiency | Escherichia coli |
| M147I | mutation in subunit DmsA. About 65% of wild-type catalytic efficiency | Escherichia coli |
| M147L | mutation in subunit DmsA. About 50% of wild-type catalytic efficiency | Escherichia coli |
| Q179I | mutation in subunit DmsA. About 500% of wild-type catalytic efficiency | Escherichia coli |
| Q179I | mutation in subunit DmsA. Mutant is functionally impairment, with abnormal anaerobic growth with dimethylsulfoxide as the sole terminal acceptor, in a recombinant strain deleted for chromosomal dmsABC | Escherichia coli |
| R149C | mutation in subunit DmsA. About 50% of wild-type catalytic efficiency | Escherichia coli |
| R217Q | mutation in subunit DmsA. About 2.7% of wild-type catalytic efficiency | Escherichia coli |
| R217Q | mutation in subunit DmsA. Mutant is functionally impairment, with abnormal anaerobic growth with dimethylsulfoxide as the sole terminal acceptor, in a recombinant strain deleted for chromosomal dmsABC | Escherichia coli |
| T148S | mutation in subunit DmsA. About 150% of wild-type catalytic efficiency | Escherichia coli |
| T148S | mutation in subunit DmsA. Mutant shows altered kinetic parameters for pyridine N-oxide and dimethylsulfoxide, with Km and kcat decreasing and increasing approximately fourfold,respectively | Escherichia coli |
| W191G | mutation in subunit DmsA. About 80% of wild-type catalytic efficiency | Escherichia coli |
| W357C | mutation in subunit DmsA. About 100% of wild-type catalytic efficiency | Escherichia coli |
| W357F | mutation in subunit DmsA. About 40% of wild-type catalytic efficiency | Escherichia coli |
| W357Y | mutation in subunit DmsA. About 60% of wild-type catalytic efficiency | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.001 | - |
reduced benzyl viologen | mutant A178Q, pH not specified in the publication, temperature not specified in the publication | Escherichia coli |  |
| 0.001 | - |
Pyridine N-oxide | mutant A178Q, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 0.019 | - |
reduced benzyl viologen | mutant Q179I, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 0.019 | - |
reduced benzyl viologen | mutant W357Y, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 0.02 | - |
reduced benzyl viologen | mutant M147I, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 0.023 | - |
reduced benzyl viologen | mutant W357F, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 0.024 | - |
Pyridine N-oxide | mutant T148S, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 0.024 | - |
reduced benzyl viologen | mutant W357C, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 0.028 | - |
reduced benzyl viologen | mutant W191G, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 0.028 | - |
Pyridine N-oxide | mutant W357Y, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 0.032 | - |
reduced benzyl viologen | wild-type, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 0.033 | - |
reduced benzyl viologen | mutant G190V, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 0.034 | - |
Pyridine N-oxide | mutant Q179I, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 0.038 | - |
reduced benzyl viologen | mutant G167N, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 0.038 | - |
reduced benzyl viologen | mutant G190D, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 0.039 | - |
reduced benzyl viologen | mutant A181T, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 0.04 | - |
reduced benzyl viologen | mutant M147L, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 0.048 | - |
Pyridine N-oxide | mutant W191G, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 0.048 | - |
Pyridine N-oxide | mutant W357F, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 0.076 | - |
Pyridine N-oxide | mutant G167N, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 0.076 | - |
reduced benzyl viologen | mutant R149C, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 0.095 | - |
Pyridine N-oxide | mutant W357C, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 0.1 | - |
Pyridine N-oxide | wild-type, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 0.11 | - |
Pyridine N-oxide | mutant M147L, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 0.11 | - |
reduced benzyl viologen | mutant T148S, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 0.12 | - |
Pyridine N-oxide | mutant G190V, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 0.12 | - |
Pyridine N-oxide | mutant R149C, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 0.14 | - |
Pyridine N-oxide | mutant G190D, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 0.18 | - |
Pyridine N-oxide | mutant M147I, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 0.24 | - |
Pyridine N-oxide | mutant A181T, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 1.1 | - |
reduced benzyl viologen | mutant R217Q, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 3.8 | - |
Pyridine N-oxide | mutant R217Q, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dimethylsulfide + H2O + pyridine N-oxide | - |
Escherichia coli | dimethylsulfoxide + pyridine | - |
? | |
| dimethylsulfoxide + 2,3-dimethyl-1,4-naphthoquinol | - |
Escherichia coli | dimethylsulfide + H2O + 2,3-dimethyl-1,4-naphthoquinone | - |
r | |
| dimethylsulfoxide + reduced benzyl viologen | - |
Escherichia coli | dimethylsulfide + H2O + oxidized benzyl viologen | - |
r | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3 | 6 | Pyridine N-oxide | mutant Q179I, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 10 | - |
Pyridine N-oxide | mutant A178Q, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 14 | - |
reduced benzyl viologen | mutant G167N, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 17 | - |
reduced benzyl viologen | mutant W357F, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 19 | - |
reduced benzyl viologen | mutant Q179I, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 23 | - |
reduced benzyl viologen | mutant A178Q, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 24 | - |
reduced benzyl viologen | mutant W357Y, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 27 | - |
reduced benzyl viologen | mutant M147I, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 33 | - |
Pyridine N-oxide | mutant W357Y, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 39 | - |
reduced benzyl viologen | mutant M147L, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 41 | - |
Pyridine N-oxide | mutant W357F, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 46 | - |
reduced benzyl viologen | mutant W357C, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 47 | - |
reduced benzyl viologen | mutant W191G, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 55 | - |
reduced benzyl viologen | mutant R217Q, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 60 | - |
reduced benzyl viologen | mutant G190D, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 61 | - |
reduced benzyl viologen | wild-type, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 68 | - |
reduced benzyl viologen | mutant R149C, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 95 | - |
Pyridine N-oxide | mutant R149C, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 96 | - |
Pyridine N-oxide | mutant T148S, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 99 | - |
Pyridine N-oxide | mutant G167N, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 99 | - |
Pyridine N-oxide | mutant W191G, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 100 | - |
Pyridine N-oxide | mutant W357C, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 110 | - |
reduced benzyl viologen | mutant G190V, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 110 | - |
Pyridine N-oxide | mutant M147L, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 120 | - |
Pyridine N-oxide | mutant R217Q, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 190 | - |
Pyridine N-oxide | mutant G190D, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 190 | - |
Pyridine N-oxide | mutant M147I, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 200 | - |
Pyridine N-oxide | wild-type, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 210 | - |
reduced benzyl viologen | mutant A181T, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 260 | - |
Pyridine N-oxide | mutant G190V, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 370 | - |
reduced benzyl viologen | mutant T148S, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 940 | - |
Pyridine N-oxide | mutant A181T, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 33 | - |
Pyridine N-oxide | mutant R217Q, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 50 | - |
reduced benzyl viologen | mutant R217Q, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 370 | - |
reduced benzyl viologen | mutant G167N, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 760 | - |
reduced benzyl viologen | mutant W357F, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 810 | - |
Pyridine N-oxide | mutant R149C, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 850 | - |
Pyridine N-oxide | mutant W357F, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 900 | - |
reduced benzyl viologen | mutant R149C, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 960 | - |
reduced benzyl viologen | mutant M147L, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 1000 | - |
Pyridine N-oxide | mutant M147L, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 1000 | - |
reduced benzyl viologen | mutant Q179I, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 1100 | - |
Pyridine N-oxide | mutant M147I, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 1100 | - |
Pyridine N-oxide | mutant Q179I, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 1100 | - |
Pyridine N-oxide | mutant W357C, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 1200 | - |
Pyridine N-oxide | mutant G190D, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 1200 | - |
Pyridine N-oxide | mutant W357Y, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 1300 | - |
Pyridine N-oxide | mutant G167N, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 1300 | - |
reduced benzyl viologen | mutant W357Y, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 1360 | - |
reduced benzyl viologen | mutant M147I, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 1600 | - |
reduced benzyl viologen | mutant G190D, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 1600 | - |
reduced benzyl viologen | mutant W191G, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 1900 | - |
reduced benzyl viologen | mutant W357C, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 1900 | - |
reduced benzyl viologen | wild-type, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 2000 | - |
Pyridine N-oxide | wild-type, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 2100 | - |
Pyridine N-oxide | mutant G190V, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 2100 | - |
Pyridine N-oxide | mutant W191G, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 3300 | - |
reduced benzyl viologen | mutant T148S, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 3400 | - |
reduced benzyl viologen | mutant G190V, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 4000 | - |
Pyridine N-oxide | mutant A181T, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 4000 | - |
Pyridine N-oxide | mutant T148S, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 5500 | - |
reduced benzyl viologen | mutant A181T, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 10000 | - |
Pyridine N-oxide | mutant A178Q, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 23300 | - |
reduced benzyl viologen | mutant A178Q, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
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