BRENDA - Enzyme Database show
show all sequences of 1.8.5.3

Modulation of the substrate specificity of Escherichia coli dimethylsulfoxide reductase

Simala-Grant, J.L.; Weiner, J.H.; Eur. J. Biochem. 251, 510-515 (1998)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
A178Q
mutation in subunit DmsA. About 1200% of wild-type catalytic efficiency; mutation in subunit DmsA. Mutant is functionally impairment, with abnormal anaerobic growth with dimethylsulfoxide as the sole terminal acceptor, in a recombinant strain deleted for chromosomal dmsABC
Escherichia coli
A181T
mutation in subunit DmsA. About 300% of wild-type catalytic efficiency
Escherichia coli
G167N
mutation in subunit DmsA. About 20% of wild-type catalytic efficiency; mutation in subunit DmsA. Mutant is functionally impairment, with abnormal anaerobic growth with dimethylsulfoxide as the sole terminal acceptor, in a recombinant strain deleted for chromosomal dmsABC
Escherichia coli
G190D
mutation in subunit DmsA. About 80% of wild-type catalytic efficiency
Escherichia coli
G190V
mutation in subunit DmsA. About 180% of wild-type catalytic efficiency
Escherichia coli
M147I
mutation in subunit DmsA. About 65% of wild-type catalytic efficiency
Escherichia coli
M147L
mutation in subunit DmsA. About 50% of wild-type catalytic efficiency
Escherichia coli
Q179I
mutation in subunit DmsA. About 500% of wild-type catalytic efficiency; mutation in subunit DmsA. Mutant is functionally impairment, with abnormal anaerobic growth with dimethylsulfoxide as the sole terminal acceptor, in a recombinant strain deleted for chromosomal dmsABC
Escherichia coli
R149C
mutation in subunit DmsA. About 50% of wild-type catalytic efficiency
Escherichia coli
R217Q
mutation in subunit DmsA. About 2.7% of wild-type catalytic efficiency; mutation in subunit DmsA. Mutant is functionally impairment, with abnormal anaerobic growth with dimethylsulfoxide as the sole terminal acceptor, in a recombinant strain deleted for chromosomal dmsABC
Escherichia coli
T148S
mutation in subunit DmsA. About 150% of wild-type catalytic efficiency; mutation in subunit DmsA. Mutant shows altered kinetic parameters for pyridine N-oxide and dimethylsulfoxide, with Km and kcat decreasing and increasing approximately fourfold,respectively
Escherichia coli
W191G
mutation in subunit DmsA. About 80% of wild-type catalytic efficiency
Escherichia coli
W357C
mutation in subunit DmsA. About 100% of wild-type catalytic efficiency
Escherichia coli
W357F
mutation in subunit DmsA. About 40% of wild-type catalytic efficiency
Escherichia coli
W357Y
mutation in subunit DmsA. About 60% of wild-type catalytic efficiency
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.001
-
Pyridine N-oxide
mutant A178Q, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.001
-
reduced benzyl viologen
mutant A178Q, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.019
-
reduced benzyl viologen
mutant Q179I, pH not specified in the publication, temperature not specified in the publication; mutant W357Y, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.02
-
reduced benzyl viologen
mutant M147I, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.023
-
reduced benzyl viologen
mutant W357F, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.024
-
Pyridine N-oxide
mutant T148S, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.024
-
reduced benzyl viologen
mutant W357C, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.028
-
Pyridine N-oxide
mutant W357Y, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.028
-
reduced benzyl viologen
mutant W191G, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.032
-
reduced benzyl viologen
wild-type, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.033
-
reduced benzyl viologen
mutant G190V, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.034
-
Pyridine N-oxide
mutant Q179I, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.038
-
reduced benzyl viologen
mutant G167N, pH not specified in the publication, temperature not specified in the publication; mutant G190D, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.039
-
reduced benzyl viologen
mutant A181T, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.04
-
reduced benzyl viologen
mutant M147L, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.048
-
Pyridine N-oxide
mutant W191G, pH not specified in the publication, temperature not specified in the publication; mutant W357F, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.076
-
Pyridine N-oxide
mutant G167N, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.076
-
reduced benzyl viologen
mutant R149C, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.095
-
Pyridine N-oxide
mutant W357C, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.1
-
Pyridine N-oxide
wild-type, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.11
-
Pyridine N-oxide
mutant M147L, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.11
-
reduced benzyl viologen
mutant T148S, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.12
-
Pyridine N-oxide
mutant G190V, pH not specified in the publication, temperature not specified in the publication; mutant R149C, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.14
-
Pyridine N-oxide
mutant G190D, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.18
-
Pyridine N-oxide
mutant M147I, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.24
-
Pyridine N-oxide
mutant A181T, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
1.1
-
reduced benzyl viologen
mutant R217Q, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
3.8
-
Pyridine N-oxide
mutant R217Q, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dimethylsulfide + H2O + pyridine N-oxide
-
711886
Escherichia coli
dimethylsulfoxide + pyridine
-
-
-
?
dimethylsulfoxide + 2,3-dimethyl-1,4-napthoquinol
-
711886
Escherichia coli
dimethylsulfide + H2O + 2,3-dimethyl-1,4-napthoquinone
-
-
-
r
dimethylsulfoxide + reduced benzyl viologen
-
711886
Escherichia coli
dimethylsulfide + H2O + oxidized benzyl viologen
-
-
-
r
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3
6
Pyridine N-oxide
mutant Q179I, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
10
-
Pyridine N-oxide
mutant A178Q, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
14
-
reduced benzyl viologen
mutant G167N, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
17
-
reduced benzyl viologen
mutant W357F, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
19
-
reduced benzyl viologen
mutant Q179I, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
23
-
reduced benzyl viologen
mutant A178Q, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
24
-
reduced benzyl viologen
mutant W357Y, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
27
-
reduced benzyl viologen
mutant M147I, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
33
-
Pyridine N-oxide
mutant W357Y, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
39
-
reduced benzyl viologen
mutant M147L, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
41
-
Pyridine N-oxide
mutant W357F, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
46
-
reduced benzyl viologen
mutant W357C, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
47
-
reduced benzyl viologen
mutant W191G, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
55
-
reduced benzyl viologen
mutant R217Q, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
60
-
reduced benzyl viologen
mutant G190D, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
61
-
reduced benzyl viologen
wild-type, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
68
-
reduced benzyl viologen
mutant R149C, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
95
-
Pyridine N-oxide
mutant R149C, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
96
-
Pyridine N-oxide
mutant T148S, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
99
-
Pyridine N-oxide
mutant G167N, pH not specified in the publication, temperature not specified in the publication; mutant W191G, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
100
-
Pyridine N-oxide
mutant W357C, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
110
-
Pyridine N-oxide
mutant M147L, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
110
-
reduced benzyl viologen
mutant G190V, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
120
-
Pyridine N-oxide
mutant R217Q, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
190
-
Pyridine N-oxide
mutant G190D, pH not specified in the publication, temperature not specified in the publication; mutant M147I, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
200
-
Pyridine N-oxide
wild-type, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
210
-
reduced benzyl viologen
mutant A181T, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
260
-
Pyridine N-oxide
mutant G190V, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
370
-
reduced benzyl viologen
mutant T148S, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
940
-
Pyridine N-oxide
mutant A181T, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
A178Q
mutation in subunit DmsA. About 1200% of wild-type catalytic efficiency; mutation in subunit DmsA. Mutant is functionally impairment, with abnormal anaerobic growth with dimethylsulfoxide as the sole terminal acceptor, in a recombinant strain deleted for chromosomal dmsABC
Escherichia coli
A181T
mutation in subunit DmsA. About 300% of wild-type catalytic efficiency
Escherichia coli
G167N
mutation in subunit DmsA. About 20% of wild-type catalytic efficiency; mutation in subunit DmsA. Mutant is functionally impairment, with abnormal anaerobic growth with dimethylsulfoxide as the sole terminal acceptor, in a recombinant strain deleted for chromosomal dmsABC
Escherichia coli
G190D
mutation in subunit DmsA. About 80% of wild-type catalytic efficiency
Escherichia coli
G190V
mutation in subunit DmsA. About 180% of wild-type catalytic efficiency
Escherichia coli
M147I
mutation in subunit DmsA. About 65% of wild-type catalytic efficiency
Escherichia coli
M147L
mutation in subunit DmsA. About 50% of wild-type catalytic efficiency
Escherichia coli
Q179I
mutation in subunit DmsA. About 500% of wild-type catalytic efficiency; mutation in subunit DmsA. Mutant is functionally impairment, with abnormal anaerobic growth with dimethylsulfoxide as the sole terminal acceptor, in a recombinant strain deleted for chromosomal dmsABC
Escherichia coli
R149C
mutation in subunit DmsA. About 50% of wild-type catalytic efficiency
Escherichia coli
R217Q
mutation in subunit DmsA. About 2.7% of wild-type catalytic efficiency; mutation in subunit DmsA. Mutant is functionally impairment, with abnormal anaerobic growth with dimethylsulfoxide as the sole terminal acceptor, in a recombinant strain deleted for chromosomal dmsABC
Escherichia coli
T148S
mutation in subunit DmsA. About 150% of wild-type catalytic efficiency; mutation in subunit DmsA. Mutant shows altered kinetic parameters for pyridine N-oxide and dimethylsulfoxide, with Km and kcat decreasing and increasing approximately fourfold,respectively
Escherichia coli
W191G
mutation in subunit DmsA. About 80% of wild-type catalytic efficiency
Escherichia coli
W357C
mutation in subunit DmsA. About 100% of wild-type catalytic efficiency
Escherichia coli
W357F
mutation in subunit DmsA. About 40% of wild-type catalytic efficiency
Escherichia coli
W357Y
mutation in subunit DmsA. About 60% of wild-type catalytic efficiency
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.001
-
Pyridine N-oxide
mutant A178Q, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.001
-
reduced benzyl viologen
mutant A178Q, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.019
-
reduced benzyl viologen
mutant Q179I, pH not specified in the publication, temperature not specified in the publication; mutant W357Y, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.02
-
reduced benzyl viologen
mutant M147I, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.023
-
reduced benzyl viologen
mutant W357F, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.024
-
Pyridine N-oxide
mutant T148S, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.024
-
reduced benzyl viologen
mutant W357C, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.028
-
Pyridine N-oxide
mutant W357Y, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.028
-
reduced benzyl viologen
mutant W191G, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.032
-
reduced benzyl viologen
wild-type, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.033
-
reduced benzyl viologen
mutant G190V, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.034
-
Pyridine N-oxide
mutant Q179I, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.038
-
reduced benzyl viologen
mutant G167N, pH not specified in the publication, temperature not specified in the publication; mutant G190D, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.039
-
reduced benzyl viologen
mutant A181T, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.04
-
reduced benzyl viologen
mutant M147L, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.048
-
Pyridine N-oxide
mutant W191G, pH not specified in the publication, temperature not specified in the publication; mutant W357F, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.076
-
Pyridine N-oxide
mutant G167N, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.076
-
reduced benzyl viologen
mutant R149C, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.095
-
Pyridine N-oxide
mutant W357C, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.1
-
Pyridine N-oxide
wild-type, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.11
-
Pyridine N-oxide
mutant M147L, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.11
-
reduced benzyl viologen
mutant T148S, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.12
-
Pyridine N-oxide
mutant G190V, pH not specified in the publication, temperature not specified in the publication; mutant R149C, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.14
-
Pyridine N-oxide
mutant G190D, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.18
-
Pyridine N-oxide
mutant M147I, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
0.24
-
Pyridine N-oxide
mutant A181T, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
1.1
-
reduced benzyl viologen
mutant R217Q, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
3.8
-
Pyridine N-oxide
mutant R217Q, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dimethylsulfide + H2O + pyridine N-oxide
-
711886
Escherichia coli
dimethylsulfoxide + pyridine
-
-
-
?
dimethylsulfoxide + 2,3-dimethyl-1,4-napthoquinol
-
711886
Escherichia coli
dimethylsulfide + H2O + 2,3-dimethyl-1,4-napthoquinone
-
-
-
r
dimethylsulfoxide + reduced benzyl viologen
-
711886
Escherichia coli
dimethylsulfide + H2O + oxidized benzyl viologen
-
-
-
r
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3
6
Pyridine N-oxide
mutant Q179I, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
10
-
Pyridine N-oxide
mutant A178Q, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
14
-
reduced benzyl viologen
mutant G167N, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
17
-
reduced benzyl viologen
mutant W357F, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
19
-
reduced benzyl viologen
mutant Q179I, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
23
-
reduced benzyl viologen
mutant A178Q, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
24
-
reduced benzyl viologen
mutant W357Y, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
27
-
reduced benzyl viologen
mutant M147I, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
33
-
Pyridine N-oxide
mutant W357Y, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
39
-
reduced benzyl viologen
mutant M147L, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
41
-
Pyridine N-oxide
mutant W357F, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
46
-
reduced benzyl viologen
mutant W357C, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
47
-
reduced benzyl viologen
mutant W191G, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
55
-
reduced benzyl viologen
mutant R217Q, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
60
-
reduced benzyl viologen
mutant G190D, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
61
-
reduced benzyl viologen
wild-type, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
68
-
reduced benzyl viologen
mutant R149C, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
95
-
Pyridine N-oxide
mutant R149C, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
96
-
Pyridine N-oxide
mutant T148S, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
99
-
Pyridine N-oxide
mutant G167N, pH not specified in the publication, temperature not specified in the publication; mutant W191G, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
100
-
Pyridine N-oxide
mutant W357C, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
110
-
Pyridine N-oxide
mutant M147L, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
110
-
reduced benzyl viologen
mutant G190V, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
120
-
Pyridine N-oxide
mutant R217Q, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
190
-
Pyridine N-oxide
mutant G190D, pH not specified in the publication, temperature not specified in the publication; mutant M147I, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
200
-
Pyridine N-oxide
wild-type, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
210
-
reduced benzyl viologen
mutant A181T, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
260
-
Pyridine N-oxide
mutant G190V, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
370
-
reduced benzyl viologen
mutant T148S, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
940
-
Pyridine N-oxide
mutant A181T, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
33
-
Pyridine N-oxide
mutant R217Q, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
50
-
reduced benzyl viologen
mutant R217Q, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
370
-
reduced benzyl viologen
mutant G167N, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
760
-
reduced benzyl viologen
mutant W357F, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
810
-
Pyridine N-oxide
mutant R149C, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
850
-
Pyridine N-oxide
mutant W357F, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
900
-
reduced benzyl viologen
mutant R149C, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
960
-
reduced benzyl viologen
mutant M147L, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
1000
-
Pyridine N-oxide
mutant M147L, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
1000
-
reduced benzyl viologen
mutant Q179I, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
1100
-
Pyridine N-oxide
mutant M147I, pH not specified in the publication, temperature not specified in the publication; mutant Q179I, pH not specified in the publication, temperature not specified in the publication; mutant W357C, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
1200
-
Pyridine N-oxide
mutant G190D, pH not specified in the publication, temperature not specified in the publication; mutant W357Y, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
1300
-
Pyridine N-oxide
mutant G167N, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
1300
-
reduced benzyl viologen
mutant W357Y, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
1360
-
reduced benzyl viologen
mutant M147I, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
1600
-
reduced benzyl viologen
mutant G190D, pH not specified in the publication, temperature not specified in the publication; mutant W191G, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
1900
-
reduced benzyl viologen
mutant W357C, pH not specified in the publication, temperature not specified in the publication; wild-type, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
2000
-
Pyridine N-oxide
wild-type, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
2100
-
Pyridine N-oxide
mutant G190V, pH not specified in the publication, temperature not specified in the publication; mutant W191G, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
3300
-
reduced benzyl viologen
mutant T148S, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
3400
-
reduced benzyl viologen
mutant G190V, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
4000
-
Pyridine N-oxide
mutant A181T, pH not specified in the publication, temperature not specified in the publication; mutant T148S, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
5500
-
reduced benzyl viologen
mutant A181T, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
10000
-
Pyridine N-oxide
mutant A178Q, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
23300
-
reduced benzyl viologen
mutant A178Q, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
33
-
Pyridine N-oxide
mutant R217Q, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
50
-
reduced benzyl viologen
mutant R217Q, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
370
-
reduced benzyl viologen
mutant G167N, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
760
-
reduced benzyl viologen
mutant W357F, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
810
-
Pyridine N-oxide
mutant R149C, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
850
-
Pyridine N-oxide
mutant W357F, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
900
-
reduced benzyl viologen
mutant R149C, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
960
-
reduced benzyl viologen
mutant M147L, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
1000
-
Pyridine N-oxide
mutant M147L, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
1000
-
reduced benzyl viologen
mutant Q179I, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
1100
-
Pyridine N-oxide
mutant M147I, pH not specified in the publication, temperature not specified in the publication; mutant Q179I, pH not specified in the publication, temperature not specified in the publication; mutant W357C, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
1200
-
Pyridine N-oxide
mutant G190D, pH not specified in the publication, temperature not specified in the publication; mutant W357Y, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
1300
-
Pyridine N-oxide
mutant G167N, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
1300
-
reduced benzyl viologen
mutant W357Y, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
1360
-
reduced benzyl viologen
mutant M147I, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
1600
-
reduced benzyl viologen
mutant G190D, pH not specified in the publication, temperature not specified in the publication; mutant W191G, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
1900
-
reduced benzyl viologen
mutant W357C, pH not specified in the publication, temperature not specified in the publication; wild-type, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
2000
-
Pyridine N-oxide
wild-type, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
2100
-
Pyridine N-oxide
mutant G190V, pH not specified in the publication, temperature not specified in the publication; mutant W191G, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
3300
-
reduced benzyl viologen
mutant T148S, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
3400
-
reduced benzyl viologen
mutant G190V, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
4000
-
Pyridine N-oxide
mutant A181T, pH not specified in the publication, temperature not specified in the publication; mutant T148S, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
5500
-
reduced benzyl viologen
mutant A181T, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
10000
-
Pyridine N-oxide
mutant A178Q, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
23300
-
reduced benzyl viologen
mutant A178Q, pH not specified in the publication, temperature not specified in the publication
Escherichia coli
Other publictions for EC 1.8.5.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742743
Ha
Sulfur K-edge X-ray absorptio ...
Escherichia coli
J. Am. Chem. Soc.
136
9094-9105
2014
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
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1
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-
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-
-
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1
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-
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-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
724457
Tang
A variant conferring cofactor- ...
Escherichia coli
Biochim. Biophys. Acta
1827
730-737
2013
-
-
-
-
1
-
-
-
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
725605
Li
Large density-functional and b ...
Rhodobacter capsulatus
J. Chem. Theory Comput.
9
1799-1807
2013
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
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-
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-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
725419
Tang
Correct assembly of iron-sulfu ...
Escherichia coli
J. Biol. Chem.
286
15147-15154
2011
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
10
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
10
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
724687
Hernandez-Marin
-
A kinetic study of dimethyl su ...
Rhodobacter capsulatus
Can. J. Chem.
88
683-693
2010
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
712155
George
X-ray absorption spectroscopic ...
Escherichia coli
Inorg. Chem.
46
2-4
2007
-
-
-
1
-
-
-
-
-
1
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
712156
George
Interaction of product analogu ...
Rhodobacter sphaeroides
Inorg. Chem.
46
3097-3104
2007
-
-
1
1
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
711196
Cheng
Investigation of the environme ...
Escherichia coli
Biochemistry
44
8068-8077
2005
-
-
-
-
32
-
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
32
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
711815
McNamara
-
The nature and function of the ...
synthetic construct
Dalton Trans.
2005
3572-3579
2005
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
712000
Ridge
The critical role of tryptopha ...
Rhodobacter capsulatus
FEBS Lett.
563
197-202
2004
-
-
-
-
1
-
-
4
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
4
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
4
-
-
-
-
-
-
-
-
4
4
712148
Baltes
Identification of dimethyl sul ...
Actinobacillus pleuropneumoniae, Actinobacillus pleuropneumoniae serotype 7
Infect. Immun.
71
6784-6792
2003
-
-
1
-
-
-
-
-
1
-
1
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
1
1
1
-
-
711193
Bray
Reactions of dimethylsulfoxide ...
Rhodobacter capsulatus
Biochemistry
40
9810-9820
2001
-
-
-
1
-
-
-
-
-
1
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
712347
Johnson
An active site tyrosine influe ...
Rhodobacter sphaeroides
J. Biol. Chem.
276
13178-13185
2001
-
-
1
-
2
-
-
12
-
-
-
-
-
3
-
-
-
-
-
-
-
-
4
-
-
-
-
12
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
12
-
-
-
-
-
-
-
-
12
12
712734
Stewart
Dimethylsulfoxide reductase: a ...
Rhodobacter capsulatus
J. Mol. Biol.
299
593-600
2000
-
-
1
1
-
-
-
-
-
1
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
711190
Adams
Reactions of dimethylsulfoxide ...
Rhodobacter capsulatus
Biochemistry
38
8501-8511
1999
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
5
-
-
-
-
3
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
3
2
-
-
-
-
-
-
-
-
-
712341
Rothery
Interactions between the molyb ...
Escherichia coli
J. Biol. Chem.
274
13002-13009
1999
-
-
-
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
711886
Simala-Grant
Modulation of the substrate sp ...
Escherichia coli
Eur. J. Biochem.
251
510-515
1998
-
-
-
-
15
-
-
28
-
-
-
-
-
4
-
-
-
-
-
-
-
-
3
-
-
-
-
30
-
-
-
-
-
-
-
-
-
-
-
-
15
-
-
-
-
28
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
30
-
-
-
-
-
-
-
-
25
25
712339
Zhao
Interaction of 2-n-heptyl-4-hy ...
Escherichia coli
J. Biol. Chem.
273
20758-20763
1998
-
-
-
-
1
-
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
712960
Hanlon
Asymmetric reduction of racemi ...
Escherichia coli, Proteus mirabilis, Rhodobacter capsulatus
Microbiology
144
2247-2253
1998
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
20
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
20
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
711885
Solomon
Characterisation of the pterin ...
Rhodobacter capsulatus
Eur. J. Biochem.
246
200-203
1997
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
713451
Turner
Expression and epitope tagging ...
Escherichia coli
Protein Eng.
10
285-290
1997
-
-
-
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
675504
Schneider
Crystal structure of dimethyl ...
Rhodobacter capsulatus
J. Mol. Biol.
263
53-69
1996
-
-
-
1
-
-
-
-
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
710991
Hilton
Identification of the molybden ...
Rhodobacter sphaeroides, Rhodobacter sphaeroides f. sp. denitrificans
Arch. Biochem. Biophys.
325
139-143
1996
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
712959
Simala-Grant
Kinetic analysis and substrate ...
Escherichia coli
Microbiology
142
3231-3239
1996
-
-
-
-
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20
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2
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25
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25
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20
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25
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25
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1
1
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20
20
713506
Schindelin
Crystal structure of DMSO redu ...
Rhodobacter sphaeroides
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1615-1621
1996
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1
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2
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1
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1
1
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711479
Abo
Enantioselective deoxygenation ...
Rhodobacter sphaeroides, Rhodobacter sphaeroides f.s. denitrificans
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109-112
1995
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4
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14
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14
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710783
Fischer
Molybdenum-pterin complexes: A ...
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338
369-372
1993
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1
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713250
Masui
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Accumulation on the cytoplasmi ...
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463-469
1992
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1
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3
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1
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1
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1
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1
1
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711803
Miguel
-
Electron donors and the quinon ...
Escherichia coli
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22
109-115
1991
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1
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1
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1
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1
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1
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1
1
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-
712043
Daruwala
Dimethyl sulfoxide reductase i ...
Escherichia coli
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83
255-259
1991
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2
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1
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1
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1
1
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712267
Sambasivarao
Dimethyl sulfoxide reductase o ...
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5935-5943
1991
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1
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1
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2
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1
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1
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1
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1
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