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Literature summary for 1.8.5.1 extracted from
- Purification and characterization of dehydroascorbate reductase from rice (1997), Plant Cell Physiol., 38, 173-178.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | 5 mM, enhances the activity by 13% | Oryza sativa |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| iodoacetic acid | 0.1 mM, 48% loss of activity. 1.0 mM, 91% loss of activity | Oryza sativa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.35 | - |
dehydroascorbate | - |
Oryza sativa | |
| 0.84 | - |
GSH | - |
Oryza sativa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26000 | - |
1 * 26000, SDS-PAGE | Oryza sativa |
| 27000 | - |
gel filtration | Oryza sativa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione + dehydroascorbate | Oryza sativa | the enzyme is critical for maintenance of an appropriate level of ascorbate in plant cells | GSSG + ascorbate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryza sativa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Oryza sativa |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 49.1 | - |
- |
Oryza sativa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione + dehydroascorbate | the enzyme is critical for maintenance of an appropriate level of ascorbate in plant cells | Oryza sativa | GSSG + ascorbate | - |
? | |
| GSH + dehydroascorbate | - |
Oryza sativa | GSSG + ascorbate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 26000, SDS-PAGE | Oryza sativa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
10 min, 12% loss of activity | Oryza sativa |
| 80 | - |
10 min, 10% loss of activity | Oryza sativa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.2 | - |
- |
Oryza sativa |
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Release 2023.1 (January 2023)
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