Literature summary for 1.8.4.9 extracted from

Suter, M.; von Ballmoos, P.; Kopriva, S.; Op den Camp, R.; Schaller, J.; Kuhlemeier, C.; Schurmann, P.; Brunold, C.
Adenosine 5'-phosphosulfate sulfotransferase and adenosine 5'-phosphosulfate reductase are identical enzymes (2000), J. Biol. Chem., 275, 930-936.

Search for more literature for 1.8.4.9

Cloned(Commentary)

Cloned (Comment)	Organism
-	Lemna minor

General Stability

General Stability	Organism
enzyme unstable during purification and storage, addition of 5 mM 5'-adenosine monophosphate stabilizes activity	Lemna minor

Inhibitors

Inhibitors	Comment	Organism	Structure
5'-adenylylsulfate	at 0.12 mM	Lemna minor
adenosine-5'-phosphate	competitive inhibition, Ki of 0.34 mM with 10 mM glutathione	Lemna minor
glutathione	at 60 mM	Lemna minor

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0065	-	5'-adenylylsulfate	with 10 mM glutathione as reductant	Lemna minor
0.0122	-	5'-adenylylsulfate	with 40 mM glutathione as reductant	Lemna minor
16.8	-	glutathione	with 0.04 mM adenylylsulfate	Lemna minor

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
chloroplast	sequence contains chloroplast transit peptide	Lemna minor	9507	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
43000	-	SDS-PAGE	Lemna minor
43640	-	calculated from cDNA sequence	Lemna minor
91300	-	estimation by gel filtration with Superose 12	Lemna minor

Organism

Organism	UniProt	Comment	Textmining
Lemna minor	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
purification to homogeneity	Lemna minor

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.02	0.025	crude extract, plants treated with 10 mM NH4Cl	Lemna minor

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3'-phosphoadenosine-5'-phosphosulfate + glutathione	no activity with 3'-phosphoadenosine-5'-phosphosulfate	Lemna minor	adenosine 3',5'-diphosphate + sulfite + oxidized glutathione	-	?
5'-adenylylsulfate + dithioerythritol	-	Lemna minor	adenosine 5'-monophosphate + sulfite + oxidized dithioerythritol	-	?
5'-adenylylsulfate + glutathione	-	Lemna minor	adenosine monophosphate + sulfite + oxidized glutathione	-	?

Subunits

Subunits	Comment	Organism
dimer	alpha2, 2 * 43000, comparison of native mass after gel filtration with denatured after SDS-PAGE	Lemna minor

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
56	-	5'-adenylylsulfate	-	Lemna minor

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Release 2023.1 (January 2023)