BRENDA - Enzyme Database show
show all sequences of 1.8.4.9

Adenosine 5'-phosphosulfate sulfotransferase and adenosine 5'-phosphosulfate reductase are identical enzymes

Suter, M.; von Ballmoos, P.; Kopriva, S.; Op den Camp, R.; Schaller, J.; Kuhlemeier, C.; Schurmann, P.; Brunold, C.; J. Biol. Chem. 275, 930-936 (2000)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
-
Lemna minor
General Stability
General Stability
Organism
enzyme unstable during purification and storage, addition of 5 mM 5'-adenosine monophosphate stabilizes activity
Lemna minor
Inhibitors
Inhibitors
Commentary
Organism
Structure
5'-adenylylsulfate
at 0.12 mM
Lemna minor
adenosine-5'-phosphate
competitive inhibition, Ki of 0.34 mM with 10 mM glutathione
Lemna minor
glutathione
at 60 mM
Lemna minor
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0065
-
5'-adenylylsulfate
with 10 mM glutathione as reductant
Lemna minor
0.0122
-
5'-adenylylsulfate
with 40 mM glutathione as reductant
Lemna minor
16.8
-
glutathione
with 0.04 mM adenylylsulfate
Lemna minor
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
sequence contains chloroplast transit peptide
Lemna minor
9507
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
43000
-
SDS-PAGE
Lemna minor
43640
-
calculated from cDNA sequence
Lemna minor
91300
-
estimation by gel filtration with Superose 12
Lemna minor
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Lemna minor
-
-
-
Purification (Commentary)
Commentary
Organism
purification to homogeneity
Lemna minor
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
0.02
0.025
crude extract, plants treated with 10 mM NH4Cl
Lemna minor
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3'-phosphoadenosine-5'-phosphosulfate + glutathione
no activity with 3'-phosphoadenosine-5'-phosphosulfate
288644
Lemna minor
adenosine 3',5'-diphosphate + sulfite + oxidized glutathione
-
-
-
-
5'-adenylylsulfate + dithioerythritol
-
288644
Lemna minor
adenosine 5'-monophosphate + sulfite + oxidized dithioerythritol
-
-
-
?
5'-adenylylsulfate + glutathione
-
288644
Lemna minor
adenosine monophosphate + sulfite + oxidized glutathione
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
alpha2, 2 * 43000, comparison of native mass after gel filtration with denatured after SDS-PAGE
Lemna minor
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
56
-
5'-adenylylsulfate
-
Lemna minor
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Lemna minor
General Stability (protein specific)
General Stability
Organism
enzyme unstable during purification and storage, addition of 5 mM 5'-adenosine monophosphate stabilizes activity
Lemna minor
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
5'-adenylylsulfate
at 0.12 mM
Lemna minor
adenosine-5'-phosphate
competitive inhibition, Ki of 0.34 mM with 10 mM glutathione
Lemna minor
glutathione
at 60 mM
Lemna minor
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0065
-
5'-adenylylsulfate
with 10 mM glutathione as reductant
Lemna minor
0.0122
-
5'-adenylylsulfate
with 40 mM glutathione as reductant
Lemna minor
16.8
-
glutathione
with 0.04 mM adenylylsulfate
Lemna minor
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
sequence contains chloroplast transit peptide
Lemna minor
9507
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
43000
-
SDS-PAGE
Lemna minor
43640
-
calculated from cDNA sequence
Lemna minor
91300
-
estimation by gel filtration with Superose 12
Lemna minor
Purification (Commentary) (protein specific)
Commentary
Organism
purification to homogeneity
Lemna minor
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
0.02
0.025
crude extract, plants treated with 10 mM NH4Cl
Lemna minor
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3'-phosphoadenosine-5'-phosphosulfate + glutathione
no activity with 3'-phosphoadenosine-5'-phosphosulfate
288644
Lemna minor
adenosine 3',5'-diphosphate + sulfite + oxidized glutathione
-
-
-
-
5'-adenylylsulfate + dithioerythritol
-
288644
Lemna minor
adenosine 5'-monophosphate + sulfite + oxidized dithioerythritol
-
-
-
?
5'-adenylylsulfate + glutathione
-
288644
Lemna minor
adenosine monophosphate + sulfite + oxidized glutathione
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
alpha2, 2 * 43000, comparison of native mass after gel filtration with denatured after SDS-PAGE
Lemna minor
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
56
-
5'-adenylylsulfate
-
Lemna minor
Other publictions for EC 1.8.4.9
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743157
Chung
-
The Arabidopsis thaliana aden ...
Arabidopsis thaliana
J. Plant Biol.
58
128-136
2015
-
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1
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1
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1
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1
1
1
1
-
-
689431
Phartiyal
The role of 5-adenylylsulfate ...
Glycine max
Phytochemistry
69
356-364
2008
-
-
1
-
-
-
2
4
-
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1
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1
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1
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2
1
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2
1
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4
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1
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1
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2
1
4
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1
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1
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2
1
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2
1
-
-
-
4
-
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-
-
694712
Koprivova
Complex signaling network in r ...
Arabidopsis thaliana
Plant Physiol.
146
1408-1420
2008
-
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-
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1
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1
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2
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1
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1
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2
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1
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2
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667746
Kim
The two-domain structure of 5- ...
Ulva intestinalis
Biochemistry
46
591-601
2007
-
-
1
-
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-
-
2
-
-
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4
-
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1
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1
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1
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2
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1
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1
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-
668650
Duperron
Evidence for chemoautotrophic ...
bacteria endosymbiont of Lucinoma aff. kazani
FEMS Microbiol. Ecol.
59
64-70
2007
-
-
1
-
-
-
-
-
-
-
-
-
-
1
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1
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1
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1
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-
-
687585
Kopriva
The putative moss 3'-phosphoad ...
Physcomitrella patens
J. Biol. Chem.
282
22930-22938
2007
-
-
1
-
-
-
3
2
1
7
2
2
-
8
-
-
1
1
-
-
-
4
8
1
1
-
-
2
2
-
-
2
1
-
-
-
-
1
2
-
-
-
-
3
1
2
1
7
2
2
-
-
-
1
-
-
-
4
8
1
1
-
-
2
2
-
-
-
-
-
-
-
-
-
694695
Wiedemann
The role of the novel adenosin ...
Physcomitrella patens
Plant Mol. Biol.
65
667-676
2007
-
-
1
-
1
-
-
-
-
2
-
1
-
1
-
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-
-
-
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-
2
-
1
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1
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2
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1
2
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1
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2
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1
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2
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1
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1
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-
667703
Sun
Channeling in sulfate activati ...
Mycobacterium tuberculosis
Biochemistry
45
11304-11311
2006
-
-
1
-
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-
-
2
-
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-
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1
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1
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2
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2
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1
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2
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1
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2
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-
2
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-
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-
667717
Schiffer
Reaction mechanism of the iron ...
Archaeoglobus fulgidus
Biochemistry
45
2960-2967
2006
-
-
-
1
-
-
-
-
-
-
2
-
-
2
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1
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1
1
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1
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2
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1
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1
1
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-
-
-
669891
Chartron
Substrate recognition, protein ...
Pseudomonas aeruginosa
J. Mol. Biol.
364
152-169
2006
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-
-
1
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1
2
-
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1
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1
1
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1
2
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1
1
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-
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1
2
2
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-
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-
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-
1
1
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-
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670319
Senaratne
5-Adenosinephosphosulphate red ...
Mycobacterium tuberculosis
Mol. Microbiol.
59
1744-1753
2006
-
-
-
-
-
-
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-
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1
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1
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1
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-
660168
Tsakraklides
Sulfate reduction is increased ...
Arabidopsis thaliana, Pseudomonas aeruginosa
Plant J.
32
879-889
2002
1
-
1
-
2
1
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-
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-
1
3
-
2
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-
3
-
5
1
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-
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4
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1
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1
4
-
2
1
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1
3
-
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-
-
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-
3
-
5
1
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-
660113
Kopriva
-
Influence of chilling stress o ...
Zea mays
Plant Biol.
3
24-31
2001
1
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
5
-
-
1
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-
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-
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-
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1
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1
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5
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1
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-
288643
Gao
Characterization of sulfate as ...
Arabidopsis thaliana, Brassica juncea, Conticribra weissflogii, Dunaliella salina, Emiliania huxleyi, Heterocapsa triquetra, Isochrysis galbana, Tetraselmis sp., Thalassiosira oceanica, Ulva intestinalis
Plant Physiol.
123
1087-1096
2000
-
-
1
-
-
-
-
2
-
-
1
-
-
10
-
-
-
-
-
-
12
-
20
-
1
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1
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2
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1
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12
-
20
-
1
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-
288644
Suter
Adenosine 5'-phosphosulfate su ...
Lemna minor
J. Biol. Chem.
275
930-936
2000
-
-
1
-
-
1
3
3
1
-
3
-
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1
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1
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1
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3
1
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1
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1
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1
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3
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3
1
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3
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1
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1
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3
1
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1
-
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-
288642
Prior
Structural and kinetic propert ...
Catharanthus roseus
Biochim. Biophys. Acta
1430
25-38
1999
1
-
1
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4
1
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2
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1
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3
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2
1
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1
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1
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4
1
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2
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3
-
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2
1
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660399
Bick
Glutaredoxin function for the ...
Arabidopsis thaliana
Proc. Natl. Acad. Sci. USA
95
8404-8409
1998
-
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1
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1
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1
3
-
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1
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3
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1
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1
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7
1
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1
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1
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2
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-
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1
2
-
1
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1
-
3
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1
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1
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1
-
7
1
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1
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1
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-
288640
Setya
Sulfate reduction in higher pl ...
Arabidopsis thaliana
Proc. Natl. Acad. Sci. USA
93
13383-13388
1996
3
-
1
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1
-
1
-
3
-
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1
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-
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2
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3
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1
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3
-
1
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1
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1
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3
-
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2
-
3
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1
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288641
Gutierrez-Marcos
Three members of a novel small ...
Arabidopsis thaliana
Proc. Natl. Acad. Sci. USA
93
13377-13382
1996
-
-
1
-
-
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-
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1
-
4
-
-
6
-
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1
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3
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2
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1
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1
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4
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1
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3
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2
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