BRENDA - Enzyme Database show
show all sequences of 1.8.4.9

Characterization of sulfate assimilation in marine algae focusing on the enzyme 5'-adenylylsulfate reductase

Gao, Y.; Schofield, O.M.E.; Leustek, T.; Plant Physiol. 123, 1087-1096 (2000)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
-
Ulva intestinalis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0065
-
5'-adenylylsulfate
with glutathione as reductant
Ulva intestinalis
1.39
-
glutathione
with 5'-adenylylsulfate as substrate
Ulva intestinalis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45700
-
mature protein without chloroplast transit peptide, calculated from cDNA sequence
Ulva intestinalis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
-
-
-
Brassica juncea
-
indian mustard
-
Conticribra weissflogii
-
-
-
Dunaliella salina
-
-
-
Emiliania huxleyi
-
-
-
Heterocapsa triquetra
-
-
-
Isochrysis galbana
-
-
-
Tetraselmis sp.
-
-
-
Thalassiosira oceanica
-
-
-
Ulva intestinalis
-
-
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0005
0.005
crude cell lysate
Heterocapsa triquetra
0.001
-
crude cell lysate from young leaves
Brassica juncea
0.0013
-
crude cell lysate from young leaves
Arabidopsis thaliana
0.004
0.02
crude cell lysate
Ulva intestinalis
0.04
-
purified recombinant enzyme
Ulva intestinalis
0.05
0.1
crude cell lysate
Dunaliella salina
0.08
0.13
crude cell lysate
Thalassiosira oceanica
0.1
0.25
-
Isochrysis galbana
0.1
0.25
crude cell lysate
Ulva intestinalis
0.1
0.4
crude cell lysate
Emiliania huxleyi
0.1
0.5
crude cell lysate
Tetraselmis sp.
0.2
-
crude cell lysate
Conticribra weissflogii
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3'-phosphoadenosine-5'-phosphosulfate + glutathione
-
288643
Arabidopsis thaliana
adenosine 3',5'-diphosphate + sulfite + oxidized glutathione
-
-
-
-
3'-phosphoadenosine-5'-phosphosulfate + glutathione
no activity with 3'-phosphoadenosine-5'-phosphosulfate
288643
Brassica juncea
adenosine 3',5'-diphosphate + sulfite + oxidized glutathione
-
-
-
-
3'-phosphoadenosine-5'-phosphosulfate + glutathione
no activity with 3'-phosphoadenosine-5'-phosphosulfate
288643
Dunaliella salina
adenosine 3',5'-diphosphate + sulfite + oxidized glutathione
-
-
-
-
3'-phosphoadenosine-5'-phosphosulfate + glutathione
no activity with 3'-phosphoadenosine-5'-phosphosulfate
288643
Ulva intestinalis
adenosine 3',5'-diphosphate + sulfite + oxidized glutathione
-
-
-
-
3'-phosphoadenosine-5'-phosphosulfate + glutathione
no activity with 3'-phosphoadenosine-5'-phosphosulfate
288643
Tetraselmis sp.
adenosine 3',5'-diphosphate + sulfite + oxidized glutathione
-
-
-
-
3'-phosphoadenosine-5'-phosphosulfate + glutathione
no activity with 3'-phosphoadenosine-5'-phosphosulfate
288643
Conticribra weissflogii
adenosine 3',5'-diphosphate + sulfite + oxidized glutathione
-
-
-
-
3'-phosphoadenosine-5'-phosphosulfate + glutathione
no activity with 3'-phosphoadenosine-5'-phosphosulfate
288643
Thalassiosira oceanica
adenosine 3',5'-diphosphate + sulfite + oxidized glutathione
-
-
-
-
3'-phosphoadenosine-5'-phosphosulfate + glutathione
no activity with 3'-phosphoadenosine-5'-phosphosulfate
288643
Isochrysis galbana
adenosine 3',5'-diphosphate + sulfite + oxidized glutathione
-
-
-
-
3'-phosphoadenosine-5'-phosphosulfate + glutathione
no activity with 3'-phosphoadenosine-5'-phosphosulfate
288643
Emiliania huxleyi
adenosine 3',5'-diphosphate + sulfite + oxidized glutathione
-
-
-
-
3'-phosphoadenosine-5'-phosphosulfate + glutathione
no activity with 3'-phosphoadenosine-5'-phosphosulfate
288643
Heterocapsa triquetra
adenosine 3',5'-diphosphate + sulfite + oxidized glutathione
-
-
-
-
5'-adenylylsulfate + glutathione
-
288643
Arabidopsis thaliana
adenosine monophosphate + sulfite + oxidized glutathione
-
-
-
?
5'-adenylylsulfate + glutathione
-
288643
Brassica juncea
adenosine monophosphate + sulfite + oxidized glutathione
-
-
-
?
5'-adenylylsulfate + glutathione
-
288643
Dunaliella salina
adenosine monophosphate + sulfite + oxidized glutathione
-
-
-
?
5'-adenylylsulfate + glutathione
-
288643
Ulva intestinalis
adenosine monophosphate + sulfite + oxidized glutathione
-
-
-
?
5'-adenylylsulfate + glutathione
-
288643
Tetraselmis sp.
adenosine monophosphate + sulfite + oxidized glutathione
-
-
-
?
5'-adenylylsulfate + glutathione
-
288643
Conticribra weissflogii
adenosine monophosphate + sulfite + oxidized glutathione
-
-
-
?
5'-adenylylsulfate + glutathione
-
288643
Thalassiosira oceanica
adenosine monophosphate + sulfite + oxidized glutathione
-
-
-
?
5'-adenylylsulfate + glutathione
-
288643
Isochrysis galbana
adenosine monophosphate + sulfite + oxidized glutathione
-
-
-
?
5'-adenylylsulfate + glutathione
-
288643
Emiliania huxleyi
adenosine monophosphate + sulfite + oxidized glutathione
-
-
-
?
5'-adenylylsulfate + glutathione
-
288643
Heterocapsa triquetra
adenosine monophosphate + sulfite + oxidized glutathione
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
20
25
purified, recombinant enzyme, Ea 10.1 kcal per mol between 0°C and 20°C
Ulva intestinalis
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Ulva intestinalis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0065
-
5'-adenylylsulfate
with glutathione as reductant
Ulva intestinalis
1.39
-
glutathione
with 5'-adenylylsulfate as substrate
Ulva intestinalis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45700
-
mature protein without chloroplast transit peptide, calculated from cDNA sequence
Ulva intestinalis
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0005
0.005
crude cell lysate
Heterocapsa triquetra
0.001
-
crude cell lysate from young leaves
Brassica juncea
0.0013
-
crude cell lysate from young leaves
Arabidopsis thaliana
0.004
0.02
crude cell lysate
Ulva intestinalis
0.04
-
purified recombinant enzyme
Ulva intestinalis
0.05
0.1
crude cell lysate
Dunaliella salina
0.08
0.13
crude cell lysate
Thalassiosira oceanica
0.1
0.25
-
Isochrysis galbana
0.1
0.25
crude cell lysate
Ulva intestinalis
0.1
0.4
crude cell lysate
Emiliania huxleyi
0.1
0.5
crude cell lysate
Tetraselmis sp.
0.2
-
crude cell lysate
Conticribra weissflogii
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3'-phosphoadenosine-5'-phosphosulfate + glutathione
-
288643
Arabidopsis thaliana
adenosine 3',5'-diphosphate + sulfite + oxidized glutathione
-
-
-
-
3'-phosphoadenosine-5'-phosphosulfate + glutathione
no activity with 3'-phosphoadenosine-5'-phosphosulfate
288643
Brassica juncea
adenosine 3',5'-diphosphate + sulfite + oxidized glutathione
-
-
-
-
3'-phosphoadenosine-5'-phosphosulfate + glutathione
no activity with 3'-phosphoadenosine-5'-phosphosulfate
288643
Dunaliella salina
adenosine 3',5'-diphosphate + sulfite + oxidized glutathione
-
-
-
-
3'-phosphoadenosine-5'-phosphosulfate + glutathione
no activity with 3'-phosphoadenosine-5'-phosphosulfate
288643
Ulva intestinalis
adenosine 3',5'-diphosphate + sulfite + oxidized glutathione
-
-
-
-
3'-phosphoadenosine-5'-phosphosulfate + glutathione
no activity with 3'-phosphoadenosine-5'-phosphosulfate
288643
Tetraselmis sp.
adenosine 3',5'-diphosphate + sulfite + oxidized glutathione
-
-
-
-
3'-phosphoadenosine-5'-phosphosulfate + glutathione
no activity with 3'-phosphoadenosine-5'-phosphosulfate
288643
Conticribra weissflogii
adenosine 3',5'-diphosphate + sulfite + oxidized glutathione
-
-
-
-
3'-phosphoadenosine-5'-phosphosulfate + glutathione
no activity with 3'-phosphoadenosine-5'-phosphosulfate
288643
Thalassiosira oceanica
adenosine 3',5'-diphosphate + sulfite + oxidized glutathione
-
-
-
-
3'-phosphoadenosine-5'-phosphosulfate + glutathione
no activity with 3'-phosphoadenosine-5'-phosphosulfate
288643
Isochrysis galbana
adenosine 3',5'-diphosphate + sulfite + oxidized glutathione
-
-
-
-
3'-phosphoadenosine-5'-phosphosulfate + glutathione
no activity with 3'-phosphoadenosine-5'-phosphosulfate
288643
Emiliania huxleyi
adenosine 3',5'-diphosphate + sulfite + oxidized glutathione
-
-
-
-
3'-phosphoadenosine-5'-phosphosulfate + glutathione
no activity with 3'-phosphoadenosine-5'-phosphosulfate
288643
Heterocapsa triquetra
adenosine 3',5'-diphosphate + sulfite + oxidized glutathione
-
-
-
-
5'-adenylylsulfate + glutathione
-
288643
Arabidopsis thaliana
adenosine monophosphate + sulfite + oxidized glutathione
-
-
-
?
5'-adenylylsulfate + glutathione
-
288643
Brassica juncea
adenosine monophosphate + sulfite + oxidized glutathione
-
-
-
?
5'-adenylylsulfate + glutathione
-
288643
Dunaliella salina
adenosine monophosphate + sulfite + oxidized glutathione
-
-
-
?
5'-adenylylsulfate + glutathione
-
288643
Ulva intestinalis
adenosine monophosphate + sulfite + oxidized glutathione
-
-
-
?
5'-adenylylsulfate + glutathione
-
288643
Tetraselmis sp.
adenosine monophosphate + sulfite + oxidized glutathione
-
-
-
?
5'-adenylylsulfate + glutathione
-
288643
Conticribra weissflogii
adenosine monophosphate + sulfite + oxidized glutathione
-
-
-
?
5'-adenylylsulfate + glutathione
-
288643
Thalassiosira oceanica
adenosine monophosphate + sulfite + oxidized glutathione
-
-
-
?
5'-adenylylsulfate + glutathione
-
288643
Isochrysis galbana
adenosine monophosphate + sulfite + oxidized glutathione
-
-
-
?
5'-adenylylsulfate + glutathione
-
288643
Emiliania huxleyi
adenosine monophosphate + sulfite + oxidized glutathione
-
-
-
?
5'-adenylylsulfate + glutathione
-
288643
Heterocapsa triquetra
adenosine monophosphate + sulfite + oxidized glutathione
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
20
25
purified, recombinant enzyme, Ea 10.1 kcal per mol between 0°C and 20°C
Ulva intestinalis
Other publictions for EC 1.8.4.9
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743157
Chung
-
The Arabidopsis thaliana aden ...
Arabidopsis thaliana
J. Plant Biol.
58
128-136
2015
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1
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1
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1
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1
1
1
1
-
-
689431
Phartiyal
The role of 5-adenylylsulfate ...
Glycine max
Phytochemistry
69
356-364
2008
-
-
1
-
-
-
2
4
-
-
1
-
-
1
-
-
1
-
-
2
1
-
2
1
-
-
-
4
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
2
1
4
-
-
1
-
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-
-
1
-
2
1
-
2
1
-
-
-
4
-
-
-
-
-
-
-
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-
-
694712
Koprivova
Complex signaling network in r ...
Arabidopsis thaliana
Plant Physiol.
146
1408-1420
2008
-
-
-
-
1
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-
1
-
2
-
1
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1
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2
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1
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1
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1
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1
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2
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1
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2
-
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-
-
-
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-
-
-
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-
667746
Kim
The two-domain structure of 5- ...
Ulva intestinalis
Biochemistry
46
591-601
2007
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-
1
-
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-
2
-
-
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4
-
-
1
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1
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1
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2
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1
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1
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-
-
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668650
Duperron
Evidence for chemoautotrophic ...
bacteria endosymbiont of Lucinoma aff. kazani
FEMS Microbiol. Ecol.
59
64-70
2007
-
-
1
-
-
-
-
-
-
-
-
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-
1
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1
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1
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1
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687585
Kopriva
The putative moss 3'-phosphoad ...
Physcomitrella patens
J. Biol. Chem.
282
22930-22938
2007
-
-
1
-
-
-
3
2
1
7
2
2
-
8
-
-
1
1
-
-
-
4
8
1
1
-
-
2
2
-
-
2
1
-
-
-
-
1
2
-
-
-
-
3
1
2
1
7
2
2
-
-
-
1
-
-
-
4
8
1
1
-
-
2
2
-
-
-
-
-
-
-
-
-
694695
Wiedemann
The role of the novel adenosin ...
Physcomitrella patens
Plant Mol. Biol.
65
667-676
2007
-
-
1
-
1
-
-
-
-
2
-
1
-
1
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2
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1
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1
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2
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1
2
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1
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2
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1
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2
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1
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1
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667703
Sun
Channeling in sulfate activati ...
Mycobacterium tuberculosis
Biochemistry
45
11304-11311
2006
-
-
1
-
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-
2
-
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1
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1
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2
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1
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2
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2
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667717
Schiffer
Reaction mechanism of the iron ...
Archaeoglobus fulgidus
Biochemistry
45
2960-2967
2006
-
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1
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2
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2
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1
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1
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1
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1
1
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669891
Chartron
Substrate recognition, protein ...
Pseudomonas aeruginosa
J. Mol. Biol.
364
152-169
2006
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1
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1
2
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1
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1
1
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2
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1
1
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2
2
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1
1
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670319
Senaratne
5-Adenosinephosphosulphate red ...
Mycobacterium tuberculosis
Mol. Microbiol.
59
1744-1753
2006
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1
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660168
Tsakraklides
Sulfate reduction is increased ...
Arabidopsis thaliana, Pseudomonas aeruginosa
Plant J.
32
879-889
2002
1
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1
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2
1
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1
3
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2
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3
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5
1
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4
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1
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1
4
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2
1
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1
3
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3
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5
1
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660113
Kopriva
-
Influence of chilling stress o ...
Zea mays
Plant Biol.
3
24-31
2001
1
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288643
Gao
Characterization of sulfate as ...
Arabidopsis thaliana, Brassica juncea, Conticribra weissflogii, Dunaliella salina, Emiliania huxleyi, Heterocapsa triquetra, Isochrysis galbana, Tetraselmis sp., Thalassiosira oceanica, Ulva intestinalis
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288644
Suter
Adenosine 5'-phosphosulfate su ...
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288642
Prior
Structural and kinetic propert ...
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660399
Bick
Glutaredoxin function for the ...
Arabidopsis thaliana
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288640
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Sulfate reduction in higher pl ...
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288641
Gutierrez-Marcos
Three members of a novel small ...
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