Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.8.4.9 extracted from

  • Prior, A.; Uhrig, J.F.; Heins, L.; Wiesmann, A.; Lillig, C.H.; Stoltze, C.; Soll, J.; Schwenn, J.D.
    Structural and kinetic properties of adenylyl sulfate reductase from Catharanthus roseus cell cultures (1999), Biochim. Biophys. Acta, 1430, 25-38.
    View publication on PubMed
Search for more literature for 1.8.4.9

Activating Compound

Activating Compound Comment Organism Structure
sulfate optimal concentration between 350 and 500 mM Catharanthus roseus

Cloned(Commentary)

Cloned (Comment) Organism
-
 Catharanthus roseus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0025
-
 5'-adenylylsulfate with glutathione as reductant Catharanthus roseus
0.0038
-
 5'-adenylylsulfate with DTT as reductant Catharanthus roseus
0.4
-
 DTT with 5'-adenylylsulfate as substrate Catharanthus roseus
3
-
 glutathione with 5'-adenylylsulfate as substrate Catharanthus roseus

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast precursor imported in pea chloroplast in vitro Catharanthus roseus 9507
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 mature protein without chloroplast transit peptide, calculated from cDNA sequence Catharanthus roseus
51390
-
 calculated from cDNA sequence Catharanthus roseus

Organism

Organism UniProt Comment Textmining
Catharanthus roseus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-adenylylsulfate + dithioerythritol
-
 Catharanthus roseus adenosine 5'-monophosphate + sulfite + oxidized dithioerythritol
-
 ?
5'-adenylylsulfate + dithiothreitol
-
 Catharanthus roseus adenosine 5'-monophosphate + sulfite + oxidized dithiothreitol
-
 ?
5'-adenylylsulfate + glutathione
-
 Catharanthus roseus adenosine monophosphate + sulfite + oxidized glutathione
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1
-
 5'-adenylylsulfate with glutathione as reductant Catharanthus roseus
3.4
-
 5'-adenylylsulfate with DTT as reductant Catharanthus roseus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.8
-
 recombinant protein, expressed in Escherichia coli Catharanthus roseus