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Literature summary for 1.8.4.7 extracted from

  • Battelli, M.G.
    Enzymic conversion of rat liver xanthine oxidase from dehydrogenase (D form) to oxidase (O form) (1980), FEBS Lett., 113, 47-51.
    View publication on PubMed

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
Wistar
-
Rattus norvegicus Wistar
-
Wistar
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, gel filtration, ion-exchange Rattus norvegicus

Reaction

Reaction Comment Organism Reaction ID
[xanthine dehydrogenase] + glutathione disulfide = [xanthine oxidase] + 2 glutathione thioltransferase activity with cysteine as substrate. Mainly D-form of enzyme exists Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
3750 U/mg, 1 unit is defined as the amount of the enzyme producing a 1% reduction of the ration NADH formed/uric acid formed, corrected for non-enzymic conversion Rattus norvegicus

Storage Stability

Storage Stability Organism
-25°C Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
[xanthine dehydrogenase] + oxidized glutathione reverse conversion possible by dithioerythritol, not obtained in presence of GSH Rattus norvegicus [xanthine oxidase] + glutathione
-
r
[xanthine dehydrogenase] + oxidized glutathione reverse conversion possible by dithioerythritol, not obtained in presence of GSH Rattus norvegicus Wistar [xanthine oxidase] + glutathione
-
r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8.5
-
Rattus norvegicus
8.1
-
assay at Rattus norvegicus