Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
Wistar | - |
Rattus norvegicus Wistar | - |
Wistar | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, gel filtration, ion-exchange | Rattus norvegicus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
[xanthine dehydrogenase] + glutathione disulfide = [xanthine oxidase] + 2 glutathione | thioltransferase activity with cysteine as substrate. Mainly D-form of enzyme exists | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
3750 U/mg, 1 unit is defined as the amount of the enzyme producing a 1% reduction of the ration NADH formed/uric acid formed, corrected for non-enzymic conversion | Rattus norvegicus |
Storage Stability | Organism |
---|---|
-25°C | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
[xanthine dehydrogenase] + oxidized glutathione | reverse conversion possible by dithioerythritol, not obtained in presence of GSH | Rattus norvegicus | [xanthine oxidase] + glutathione | - |
r | |
[xanthine dehydrogenase] + oxidized glutathione | reverse conversion possible by dithioerythritol, not obtained in presence of GSH | Rattus norvegicus Wistar | [xanthine oxidase] + glutathione | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8.5 | - |
Rattus norvegicus |
8.1 | - |
assay at | Rattus norvegicus |