Literature summary for 1.8.4.3 extracted from

  • Chang, S.H.; Wilken, D.R.
    Participation of the unsymmetrical disulfide of coenzyme A and glutathione in an enzymatic sulfhydryl-disulfide interchange (1966), J. Biol. Chem., 241, 4251-4260.
    View publication on PubMed

General Stability

General Stability Organism
inactivation during storage is largely reversed by glutathione Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.045
-
CoA-glutathione
-
Bos taurus
0.33
-
GSH
-
Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
120000
-
gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CoA-glutathione + GSH Rattus norvegicus
-
CoA + GSSG
-
r
CoA-glutathione + GSH Bos taurus
-
CoA + GSSG
-
r

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-
pancreas
-
Rattus norvegicus
-
kidney
-
Rattus norvegicus
-
kidney
-
Bos taurus
-
heart low activity Rattus norvegicus
-
brain
-
Rattus norvegicus
-
lung
-
Rattus norvegicus
-
muscle
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.513
-
-
Bos taurus

Storage Stability

Storage Stability Organism
-12°C, 6 weeks, stable Bos taurus
-12°C, 7 months, 70% loss of activity Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information possible functions: 1. salvage of the coenzyme form of pantothenic acid which might be lost to the cell as CoA-glutathione, 2. maintenance of the free CoA-SH at cellular level Rattus norvegicus ?
-
?
additional information possible functions: 1. salvage of the coenzyme form of pantothenic acid which might be lost to the cell as CoA-glutathione, 2. maintenance of the free CoA-SH at cellular level Bos taurus ?
-
?
additional information catalyzes GSH-GSSG exchange reaction Rattus norvegicus ?
-
?
additional information catalyzes GSH-GSSG exchange reaction Bos taurus ?
-
?
CoA-glutathione + GSH
-
Rattus norvegicus CoA + GSSG
-
r
CoA-glutathione + GSH
-
Bos taurus CoA + GSSG
-
r
pantetheine-glutathione + GSH
-
Bos taurus pantetheine + GSSG
-
?
thiolethanolamine-glutathione + GSH
-
Bos taurus thioethanolamine + GSSG
-
?
cysteine-glutathione + GSH
-
Rattus norvegicus cysteine + GSSG
-
?
cysteine-glutathione + GSH
-
Bos taurus cysteine + GSSG
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Rattus norvegicus
25
-
assay at Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
-
Bos taurus

pH Range

pH Minimum pH Maximum Comment Organism
7 9 pH 7: about 30% of activity maximum, pH 9: about 75% of activity maximum Bos taurus