General Stability | Organism |
---|---|
inactivation during storage is largely reversed by glutathione | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.045 | - |
CoA-glutathione | - |
Bos taurus | |
0.33 | - |
GSH | - |
Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
120000 | - |
gel filtration | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CoA-glutathione + GSH | Rattus norvegicus | - |
CoA + GSSG | - |
r | |
CoA-glutathione + GSH | Bos taurus | - |
CoA + GSSG | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
heart | low activity | Rattus norvegicus | - |
kidney | - |
Rattus norvegicus | - |
kidney | - |
Bos taurus | - |
liver | - |
Rattus norvegicus | - |
lung | - |
Rattus norvegicus | - |
muscle | - |
Rattus norvegicus | - |
pancreas | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.513 | - |
- |
Bos taurus |
Storage Stability | Organism |
---|---|
-12°C, 6 weeks, stable | Bos taurus |
-12°C, 7 months, 70% loss of activity | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CoA-glutathione + GSH | - |
Rattus norvegicus | CoA + GSSG | - |
r | |
CoA-glutathione + GSH | - |
Bos taurus | CoA + GSSG | - |
r | |
cysteine-glutathione + GSH | - |
Rattus norvegicus | cysteine + GSSG | - |
? | |
cysteine-glutathione + GSH | - |
Bos taurus | cysteine + GSSG | - |
? | |
additional information | possible functions: 1. salvage of the coenzyme form of pantothenic acid which might be lost to the cell as CoA-glutathione, 2. maintenance of the free CoA-SH at cellular level | Rattus norvegicus | ? | - |
? | |
additional information | possible functions: 1. salvage of the coenzyme form of pantothenic acid which might be lost to the cell as CoA-glutathione, 2. maintenance of the free CoA-SH at cellular level | Bos taurus | ? | - |
? | |
additional information | catalyzes GSH-GSSG exchange reaction | Rattus norvegicus | ? | - |
? | |
additional information | catalyzes GSH-GSSG exchange reaction | Bos taurus | ? | - |
? | |
pantetheine-glutathione + GSH | - |
Bos taurus | pantetheine + GSSG | - |
? | |
thiolethanolamine-glutathione + GSH | - |
Bos taurus | thioethanolamine + GSSG | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Rattus norvegicus |
25 | - |
assay at | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
- |
Bos taurus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | pH 7: about 30% of activity maximum, pH 9: about 75% of activity maximum | Bos taurus |