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Literature summary for 1.8.4.2 extracted from
- A method for measuring disulfide reduction by cultured mammalian cells: relative contributions of glutathione-dependent and glutathione-independent mechanisms (2000), Anal. Biochem., 281, 77-86.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,3-Bis(2-chloroethyl)-1-nitrosourea | - |
Cricetulus griseus |  |
| 1,3-Bis(2-chloroethyl)-1-nitrosourea | - |
Homo sapiens | |
| arsenite | - |
Cricetulus griseus | |
| arsenite | - |
Homo sapiens | |
| Phenylarsine oxide | - |
Cricetulus griseus | |
| Phenylarsine oxide | complete inhibition at 1 mM | Homo sapiens | |
| selenium oxide | - |
Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GSH + protein disulfide | Homo sapiens | - |
GSSG + protein-dithiol | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cricetulus griseus | - |
chinese hamster | - |
| Homo sapiens | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| insulinoma cell | - |
Homo sapiens | - |
| lung | A549 carcinoma | Homo sapiens | - |
| mammary gland | MCF7 carcinoma | Homo sapiens | - |
| ovary | - |
Cricetulus griseus | - |
| prostate gland | DU145 carcinoma | Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
specific activities of different human carcinoma cell lines with different thiol and disulfide substrates | Homo sapiens |
| 0.0035 | - |
A549 carcinoma cells, substrates GSH, cystamine or cystine | Homo sapiens |
| 0.0075 | - |
A549 carcinoma cells, substrate dithiothreitol | Homo sapiens |
| 0.03 | - |
A549 carcinoma cells, substrate hydroxyethyl disulfide | Homo sapiens |
| 0.067 | - |
A549 carcinoma cells, substrate lipoate | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-lipoate + protein disulfide | - |
Homo sapiens | ? | - |
? | |
| cysteamine + protein disulfide | low activity | Homo sapiens | ? | - |
? | |
| dithiothreitol + protein disulfide | low activity | Homo sapiens | ? | - |
? | |
| GSH + protein disulfide | - |
Homo sapiens | GSSG + protein-dithiol | - |
? | |
| hydroxyethyl disulfide + protein disulfide | - |
Homo sapiens | ? | - |
? | |
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Release 2023.1 (January 2023)
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