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Literature summary for 1.8.4.16 extracted from

  • Goldstone, D.; Haebel, P.; Katzen, F.; Bader, M.; Bardwell, J.; Beckwith, J.; Metcalf, P.
    DsbC activation by the N-terminal domain of DsbD (2001), Proc. Natl. Acad. Sci. USA, 98, 9551-9556 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.8.4.16

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli DH5alpha cells Escherichia coli

Protein Variants

Protein Variants Comment Organism
C103A active site mutant Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Escherichia coli 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
 periplasmic domain DsbDalpha gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
a [protein] with reduced L-cysteine residues + thioredoxin disulfide Escherichia coli overall reaction a [protein] carrying a disulfide bond + thioredoxin
-
 ?
[DsbC protein] with reduced L-cysteine residues + thioredoxin disulfide Escherichia coli
-
 [DsbC protein] carrying a disulfide bond + thioredoxin
-
 ?
[DsbG protein] with reduced L-cysteine residues + thioredoxin disulfide Escherichia coli
-
 [DsbG protein] carrying a disulfide bond + thioredoxin
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
High Trap Ni-chelating column chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
a [protein] with reduced L-cysteine residues + thioredoxin disulfide overall reaction Escherichia coli a [protein] carrying a disulfide bond + thioredoxin
-
 ?
[DsbC protein] with reduced L-cysteine residues + thioredoxin disulfide
-
 Escherichia coli [DsbC protein] carrying a disulfide bond + thioredoxin
-
 ?
[DsbG protein] with reduced L-cysteine residues + thioredoxin disulfide
-
 Escherichia coli [DsbG protein] carrying a disulfide bond + thioredoxin
-
 ?

Subunits

Subunits Comment Organism
? x * 18000, periplasmic domain DsbDalpha, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
DsbD
-
 Escherichia coli
DsbDalpha N-terminal enzyme fragment, periplasmic domain Escherichia coli

General Information

General Information Comment Organism
metabolism the enzyme is required to maintain the functional oxidation state of DsbC and DsbG Escherichia coli