Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.8.4.16 extracted from

  • Chung, J.; Chen, T.; Missiakas, D.
    Transfer of electrons across the cytoplasmic membrane by DsbD, a membrane protein involved in thiol-disulphide exchange and protein folding in the bacterial periplasm (2000), Mol. Microbiol., 35, 1099-1109 .
    View publication on PubMed
Search for more literature for 1.8.4.16

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Protein Variants

Protein Variants Comment Organism
C103A inactive Escherichia coli
C109A inactive Escherichia coli
C163A inactive Escherichia coli
C282A inactive Escherichia coli
C464A inactive Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasmic membrane
-
 Escherichia coli
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
a [protein] carrying a disulfide bond + thioredoxin Escherichia coli overall reaction a [protein] with reduced L-cysteine residues + thioredoxin disulfide
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Mono-Q Sepharose column chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
a [protein] carrying a disulfide bond + thioredoxin overall reaction Escherichia coli a [protein] with reduced L-cysteine residues + thioredoxin disulfide
-
 ?

Subunits

Subunits Comment Organism
? x * 60000, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
DsbD
-
 Escherichia coli

General Information

General Information Comment Organism
metabolism cytoplasmic electrons donated by thioredoxin are transferred into the periplasm via the enzyme Escherichia coli