Literature summary for 1.8.4.16 extracted from

Smith, R.; Mohanty, B.; Mowlaboccus, S.; Paxman, J.; Williams, M.; Headey, S.; Wang, G.; Subedi, P.; Doak, B.; Kahler, C.; Scanlon, M.; Heras, B.
Structural and biochemical insights into the disulfide reductase mechanism of DsbD, an essential enzyme for neisserial pathogens (2018), J. Biol. Chem., 293, 16559-16571 .

Crystallization (Commentary)

Crystallization (Comment)	Organism
oxidized (ox) and reduced (red) periplasmic domains c-DsbD and n-DsbD, using 8-20% (w/v) PEG 6000, 100 mM MES (pH 6.4), and 20 mM ZnCl2 for n-DsbDox, 2.5 M ammonium sulfate and 0.1 M Tris (pH 9.1) for n-DsbDred, 5% (w/v) PEG 400 and 1.7-2.2 M citrate/citric acid (pH 7.0-7.8) for c-DsbDox, and 0.2 M zinc acetate dehydrate, 0.1 M sodium cacodylate trihydrate (pH 7.5), and 18% (w/v) PEG 8000 for c-DsbDred	Neisseria gonorrhoeae

Crystallization (Comment)

Organism

oxidized (ox) and reduced (red) periplasmic domains c-DsbD and n-DsbD, using 8-20% (w/v) PEG 6000, 100 mM MES (pH 6.4), and 20 mM ZnCl2 for n-DsbDox, 2.5 M ammonium sulfate and 0.1 M Tris (pH 9.1) for n-DsbDred, 5% (w/v) PEG 400 and 1.7-2.2 M citrate/citric acid (pH 7.0-7.8) for c-DsbDox, and 0.2 M zinc acetate dehydrate, 0.1 M sodium cacodylate trihydrate (pH 7.5), and 18% (w/v) PEG 8000 for c-DsbDred

Neisseria gonorrhoeae

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Neisseria gonorrhoeae	16020	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
a [protein] with reduced L-cysteine residues + thioredoxin disulfide	Neisseria gonorrhoeae	overall reaction	a [protein] carrying a disulfide bond + thioredoxin	-	?
[CcmG protein] carrying a disulfide bond + thioredoxin	Neisseria gonorrhoeae	-	[CcmG protein] with reduced L-cysteine residues + thioredoxin disulfide	-	?
[DsbC protein] carrying a disulfide bond + thioredoxin	Neisseria gonorrhoeae	-	[DsbC protein] with reduced L-cysteine residues + thioredoxin disulfide	-	?
[DsbG protein] carrying a disulfide bond + thioredoxin	Neisseria gonorrhoeae	-	[DsbG protein] with reduced L-cysteine residues + thioredoxin disulfide	-	?

Organism

Organism	UniProt	Comment	Textmining
Neisseria gonorrhoeae	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
a [protein] with reduced L-cysteine residues + thioredoxin disulfide	overall reaction	Neisseria gonorrhoeae	a [protein] carrying a disulfide bond + thioredoxin	-	?
CQQGFDGTQNSCK peptide containing amide-coupled tetraazacyclododecane-1,4,7,10-tetraacetic acid + GSSG	-	Neisseria gonorrhoeae	?	-	?
human insulin + reduced dithiothreitol	-	Neisseria gonorrhoeae	?	-	?
[CcmG protein] carrying a disulfide bond + thioredoxin	-	Neisseria gonorrhoeae	[CcmG protein] with reduced L-cysteine residues + thioredoxin disulfide	-	?
[DsbC protein] carrying a disulfide bond + thioredoxin	-	Neisseria gonorrhoeae	[DsbC protein] with reduced L-cysteine residues + thioredoxin disulfide	-	?
[DsbG protein] carrying a disulfide bond + thioredoxin	-	Neisseria gonorrhoeae	[DsbG protein] with reduced L-cysteine residues + thioredoxin disulfide	-	?

Synonyms

Synonyms	Comment	Organism
DsbD	-	Neisseria gonorrhoeae

General Information

General Information	Comment	Organism
physiological function	the enzyme is essential for survival of Neisseria gonorrhoeae	Neisseria gonorrhoeae