Literature summary for 1.8.4.16 extracted from

Quinternet, M.; Tsan, P.; Selme, L.; Beaufils, C.; Jacob, C.; Boschi-Muller, S.; Averlant-Petit, M.; Branlant, G.; Cung, M.
Solution structure and backbone dynamics of the cysteine 103 to serine mutant of the N-terminal domain of DsbD from Neisseria meningitides (2008), Biochemistry, 47, 12710-12720 .

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Cloned(Commentary)

Cloned (Comment)	Organism
mutant C103S is expressed in Escherichia coli C41(DE3) cells	Neisseria meningitidis

Protein Variants

Protein Variants	Comment	Organism
C103S	active site mutant	Neisseria meningitidis

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Neisseria meningitidis	16020	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
[protein DsbC] carrying a disulfide bond + thioredoxin	Neisseria meningitidis	-	[protein DsbC] with reduced L-cysteine residues + thioredoxin disulfide	-	-
[protein DsbE] carrying a disulfide bond + thioredoxin	Neisseria meningitidis	-	[protein DsbE] with reduced L-cysteine residues + thioredoxin disulfide	-	-
[protein DsbG] carrying a disulfide bond + thioredoxin	Neisseria meningitidis	-	[protein DsbG] with reduced L-cysteine residues + thioredoxin disulfide	-	-

Organism

Organism	UniProt	Comment	Textmining
Neisseria meningitidis	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Q-Sepharose column chromatography, phenyl Sepharose column chromatography, and Econo-Pac 10 gel filtration	Neisseria meningitidis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
[protein DsbC] carrying a disulfide bond + thioredoxin	-	Neisseria meningitidis	[protein DsbC] with reduced L-cysteine residues + thioredoxin disulfide	-	-
[protein DsbE] carrying a disulfide bond + thioredoxin	-	Neisseria meningitidis	[protein DsbE] with reduced L-cysteine residues + thioredoxin disulfide	-	-
[protein DsbG] carrying a disulfide bond + thioredoxin	-	Neisseria meningitidis	[protein DsbG] with reduced L-cysteine residues + thioredoxin disulfide	-	-

Synonyms

Synonyms	Comment	Organism
DsbD	-	Neisseria meningitidis

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Release 2023.1 (January 2023)