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Literature summary for 1.8.4.16 extracted from

  • Goulding, C.; Sawaya, M.; Parseghian, A.; Lim, V.; Eisenberg, D.; Missiakas, D.
    Thiol-disulfide exchange in an immunoglobulin-like fold Structure of the N-terminal domain of DsbD (2002), Biochemistry, 41, 6920-6927 .
    View publication on PubMed
Search for more literature for 1.8.4.16

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli DH5alpha cells Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
using 25% (w/v) poly(ethylene glycol) 4000, 0.1 M sodium acetate, pH 4.6, and 0.2 M NH4SO4 Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Escherichia coli 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[DsbC protein] carrying a disulfide bond + thioredoxin Escherichia coli
-
 [DsbC protein] with reduced L-cysteine residues + thioredoxin disulfide
-
 ?
[DsbE protein] carrying a disulfide bond + thioredoxin Escherichia coli
-
 [DsbE protein] with reduced L-cysteine residues + thioredoxin disulfide
-
 ?
[DsbG protein] carrying a disulfide bond + thioredoxin Escherichia coli
-
 [DsbG protein] with reduced L-cysteine residues + thioredoxin disulfide
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P36655
-
-

Purification (Commentary)

Purification (Comment) Organism
Mono-Q-Sepharose column chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme does not catalyze the reduction of the disulfide of the thioredoxin-like oxidant DsbA Escherichia coli ?
-
-
[DsbC protein] carrying a disulfide bond + thioredoxin
-
 Escherichia coli [DsbC protein] with reduced L-cysteine residues + thioredoxin disulfide
-
 ?
[DsbE protein] carrying a disulfide bond + thioredoxin
-
 Escherichia coli [DsbE protein] with reduced L-cysteine residues + thioredoxin disulfide
-
 ?
[DsbG protein] carrying a disulfide bond + thioredoxin
-
 Escherichia coli [DsbG protein] with reduced L-cysteine residues + thioredoxin disulfide
-
 ?

Subunits

Subunits Comment Organism
? x * 16073, calculated from amino acid sequence Escherichia coli
? x * 16073, mass spectrometry analysis Escherichia coli

Synonyms

Synonyms Comment Organism
DsbD
-
 Escherichia coli