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Literature summary for 1.8.4.15 extracted from

  • Guddat, L.; Bardwell, J.; Martin, J.
    Crystal structures of reduced and oxidized DsbA Investigation of domain motion and thiolate stabilization (1998), Structure, 6, 757-767 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
the reduced enzyme form is crystallized by the dialysis method using 0.2 M ammonium acetate, 0.1 M sodium citrate pH 5.6, 30% (w/v) polyethylene glycol 4 K and 40 mM dithiothreitol. The oxidized enzyme form is crystallized by the hanging drop vapor diffusion method using 25% (w/v) polyethylene glycol 8 K in 0.1 M cacodylate buffer pH 6.5 Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
a [DsbA protein] carrying a disulfide bond + a [protein] with reduced L-cysteine residues Escherichia coli
-
a [DsbA protein] with reduced L-cysteine residues + a [protein] carrying a disulfide bond
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli P0AEG4
-
-

Purification (Commentary)

Purification (Comment) Organism
Mono Q column chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
a [DsbA protein] carrying a disulfide bond + a [protein] with reduced L-cysteine residues
-
Escherichia coli a [DsbA protein] with reduced L-cysteine residues + a [protein] carrying a disulfide bond
-
?

Synonyms

Synonyms Comment Organism
DsbA
-
Escherichia coli