Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
the reduced enzyme form is crystallized by the dialysis method using 0.2 M ammonium acetate, 0.1 M sodium citrate pH 5.6, 30% (w/v) polyethylene glycol 4 K and 40 mM dithiothreitol. The oxidized enzyme form is crystallized by the hanging drop vapor diffusion method using 25% (w/v) polyethylene glycol 8 K in 0.1 M cacodylate buffer pH 6.5 | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
a [DsbA protein] carrying a disulfide bond + a [protein] with reduced L-cysteine residues | Escherichia coli | - |
a [DsbA protein] with reduced L-cysteine residues + a [protein] carrying a disulfide bond | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0AEG4 | - |
- |
Purification (Comment) | Organism |
---|---|
Mono Q column chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
a [DsbA protein] carrying a disulfide bond + a [protein] with reduced L-cysteine residues | - |
Escherichia coli | a [DsbA protein] with reduced L-cysteine residues + a [protein] carrying a disulfide bond | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DsbA | - |
Escherichia coli |