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show all sequences of 1.8.4.14

Free methionine-(R)-sulfoxide reductase from Escherichia coli reveals a new GAF domain function

Lin, Z.; Johnson, L.C.; Weissbach, H.; Brot, N.; Lively, M.O.; Lowther, W.T.; Proc. Natl. Acad. Sci. USA 104, 9597-9602 (2007)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
-
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0098
-
thioredoxin
-
Escherichia coli
3.9
-
L-methionine (R)-S-oxide
-
Escherichia coli
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
P76270
-
-
Purification (Commentary)
Commentary
Organism
recombinant enzyme
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-methionine (R)-S-oxide + thioredoxin
fRMsr exhibits the highest Met-(R)-O reductase activity when all components of the NADPHTrxR-Trx system are present in the reaction. The enzyme is not able to reduce L-methionine (S)-S-oxide, Met sulfone, dimethyl sulfoxide, or MetO when present in the synthetic peptide NH2-Pro-Thr-Ser-Met-(RS)-O-Glu-His-Val-NH2. Escherichia coli fRMsr is the first GAF domain family member to show enzymatic activity
689787
Escherichia coli
L-methionine + thioredoxin disulfide + H2O
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
6.9
-
L-methionine (R)-S-oxide
-
Escherichia coli
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0098
-
thioredoxin
-
Escherichia coli
3.9
-
L-methionine (R)-S-oxide
-
Escherichia coli
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant enzyme
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-methionine (R)-S-oxide + thioredoxin
fRMsr exhibits the highest Met-(R)-O reductase activity when all components of the NADPHTrxR-Trx system are present in the reaction. The enzyme is not able to reduce L-methionine (S)-S-oxide, Met sulfone, dimethyl sulfoxide, or MetO when present in the synthetic peptide NH2-Pro-Thr-Ser-Met-(RS)-O-Glu-His-Val-NH2. Escherichia coli fRMsr is the first GAF domain family member to show enzymatic activity
689787
Escherichia coli
L-methionine + thioredoxin disulfide + H2O
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
6.9
-
L-methionine (R)-S-oxide
-
Escherichia coli
Other publictions for EC 1.8.4.14
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
726800
Kim
Structural and biochemical ana ...
Thermoplasma acidophilum
Arch. Biochem. Biophys.
2014
0000
2014
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-
1
1
3
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-
3
-
-
-
-
-
2
-
-
1
-
-
-
-
-
1
-
-
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-
3
-
-
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-
-
-
-
-
-
1
-
1
3
-
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3
-
-
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-
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-
1
-
-
-
-
1
-
-
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-
3
-
-
-
-
-
-
-
-
3
3
729109
Kim
Structural and biochemical ana ...
Thermoplasma acidophilum
Arch. Biochem. Biophys.
560
10-19
2014
-
-
1
1
3
-
-
3
-
-
1
-
-
2
1
-
1
-
-
-
-
-
1
1
1
-
-
3
1
-
-
-
-
-
-
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-
1
-
1
3
-
-
-
-
3
-
-
1
-
-
1
-
1
-
-
-
-
1
1
1
-
-
3
1
-
-
-
-
-
-
-
3
3
725400
Gruez
Structural and biochemical cha ...
Neisseria meningitidis, Neisseria meningitidis 8013
J. Biol. Chem.
285
25033-25043
2010
-
-
1
1
7
-
-
12
-
-
2
-
-
3
-
-
-
-
-
-
-
-
4
1
-
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6
-
-
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-
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-
1
-
1
7
-
-
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-
12
-
-
2
-
-
-
-
-
-
-
-
-
4
1
-
-
-
6
-
-
-
-
-
-
-
-
-
-
689787
Lin
Free methionine-(R)-sulfoxide ...
Escherichia coli
Proc. Natl. Acad. Sci. USA
104
9597-9602
2007
-
-
1
-
-
-
-
2
-
-
-
-
-
3
-
-
1
-
-
-
-
-
1
-
-
-
-
1
-
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1
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2
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1
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-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
658212
Weissbach
Methionine sulfoxide reductase ...
Escherichia coli
Biochim. Biophys. Acta
1703
203-212
2005
-
-
-
-
-
-
-
-
-
-
-
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1
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-
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2
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2
-
-
-
-
-
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660853
Etienne
A methionine sulfoxide reducta ...
Escherichia coli, Escherichia coli MC1061
Biochem. Biophys. Res. Commun.
300
378-382
2003
-
-
-
-
-
-
-
-
-
-
-
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7
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1
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4
-
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3
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3
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1
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4
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