BRENDA - Enzyme Database
show all sequences of 1.8.4.13

A specific enzymatic reduction of L(-)methionine sulfoxide and a related nonspecific reduction of disulfides

Black, S.; Harte, E.M.; Hudson, B.; Wartolofsky, L.; J. Biol. Chem. 235, 2910-2916 (1960)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
4-chloromercuribenzoate
complete inhibition at 1 mM
Saccharomyces cerevisiae
arsenite
strong inhibition
Saccharomyces cerevisiae
bovine serum albumin
-
Saccharomyces cerevisiae
DL-methionine sulfoximine
50% inhibition at 1 mM
Saccharomyces cerevisiae
hydroxyethyl disulfide
competitively inhibits the activity with L-(-)-methionine S-oxide about 2.5fold
Saccharomyces cerevisiae
iodoacetic acid
complete inhibition at 10 mM
Saccharomyces cerevisiae
L-(-)-methionine S-oxide
competitively inhibits the activity with a disulfide about 2.5fold
Saccharomyces cerevisiae
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.2
-
L-(-)-methionine S-oxide
pH 7.0, 22°C
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
L-(-)-methionine S-oxide + NADPH
Saccharomyces cerevisiae
the enzyme is specific for the L-(-)-stereoisomer
L-methionine + NADP+ + H2O
-
-
ir
additional information
Saccharomyces cerevisiae
possible pathway, overview
?
-
-
-
Organism
Organism
UniProt
Commentary
Textmining
Saccharomyces cerevisiae
-
two enzyme forms
-
Purification (Commentary)
Purification (Commentary)
Organism
two native enzyme forms by ammonium sulfate fractionation, anion exchange chromatography, and a calcium phosphate resin chromatography
Saccharomyces cerevisiae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
L-(-)-methionine S-oxide + NADPH
the enzyme is specific for the L-(-)-stereoisomer
393070
Saccharomyces cerevisiae
L-methionine + NADP+ + H2O
-
-
-
ir
L-(-)-methionine S-oxide + NADPH
the enzyme is absolutely specific for the L-(-)-stereoisomer
393070
Saccharomyces cerevisiae
L-methionine + NADP+ + H2O
-
-
-
ir
additional information
possible pathway, overview
393070
Saccharomyces cerevisiae
?
-
-
-
-
additional information
the purified enzyme may contain thioredoxin
393070
Saccharomyces cerevisiae
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
22
-
assay at room temperature
Saccharomyces cerevisiae
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Saccharomyces cerevisiae
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
specific for
Saccharomyces cerevisiae
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
specific for
Saccharomyces cerevisiae
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
4-chloromercuribenzoate
complete inhibition at 1 mM
Saccharomyces cerevisiae
arsenite
strong inhibition
Saccharomyces cerevisiae
bovine serum albumin
-
Saccharomyces cerevisiae
DL-methionine sulfoximine
50% inhibition at 1 mM
Saccharomyces cerevisiae
hydroxyethyl disulfide
competitively inhibits the activity with L-(-)-methionine S-oxide about 2.5fold
Saccharomyces cerevisiae
iodoacetic acid
complete inhibition at 10 mM
Saccharomyces cerevisiae
L-(-)-methionine S-oxide
competitively inhibits the activity with a disulfide about 2.5fold
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.2
-
L-(-)-methionine S-oxide
pH 7.0, 22°C
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
L-(-)-methionine S-oxide + NADPH
Saccharomyces cerevisiae
the enzyme is specific for the L-(-)-stereoisomer
L-methionine + NADP+ + H2O
-
-
ir
additional information
Saccharomyces cerevisiae
possible pathway, overview
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
two native enzyme forms by ammonium sulfate fractionation, anion exchange chromatography, and a calcium phosphate resin chromatography
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
L-(-)-methionine S-oxide + NADPH
the enzyme is specific for the L-(-)-stereoisomer
393070
Saccharomyces cerevisiae
L-methionine + NADP+ + H2O
-
-
-
ir
L-(-)-methionine S-oxide + NADPH
the enzyme is absolutely specific for the L-(-)-stereoisomer
393070
Saccharomyces cerevisiae
L-methionine + NADP+ + H2O
-
-
-
ir
additional information
possible pathway, overview
393070
Saccharomyces cerevisiae
?
-
-
-
-
additional information
the purified enzyme may contain thioredoxin
393070
Saccharomyces cerevisiae
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
22
-
assay at room temperature
Saccharomyces cerevisiae
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Saccharomyces cerevisiae
Other publictions for EC 1.8.4.13
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
658212
Weissbach
Methionine sulfoxide reductase ...
Escherichia coli
Biochim. Biophys. Acta
1703
203-212
2005
-
1
-
-
-
-
-
-
1
-
-
3
-
1
-
-
-
-
-
-
1
-
9
-
1
-
-
-
-
-
-
-
2
-
-
-
-
1
-
2
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
1
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
393068
Ejiri
The purification of methionine ...
Escherichia coli, Escherichia coli B
Anal. Biochem.
102
393-398
1980
-
-
-
-
-
-
-
1
-
1
1
1
-
2
-
-
1
-
-
-
1
-
3
1
2
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
1
1
-
-
-
1
-
-
1
-
3
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
393071
Ejiri
Reduction of methionine sulfox ...
Escherichia coli K-12 2276, Escherichia coli K-12
J. Bacteriol.
139
161-164
1979
-
-
-
-
-
-
-
-
2
-
-
2
-
2
-
-
1
-
-
-
-
-
4
-
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
2
-
-
-
1
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
393070
Black
-
A specific enzymatic reduction ...
Saccharomyces cerevisiae
J. Biol. Chem.
235
2910-2916
1960
-
-
-
-
-
-
7
1
-
-
-
2
-
1
-
-
1
-
-
-
-
-
4
-
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
7
-
1
-
-
-
2
-
-
-
1
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-