BRENDA - Enzyme Database
show all sequences of 1.8.4.13

The purification of methionine sulfoxide reductase from Escherichia coli

Ejiri, S.; Weissbach, H.; Brot, N.; Anal. Biochem. 102, 393-398 (1980)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0003
-
L-methionine (S)-S-oxide
pH 7.5, 37C
Escherichia coli B
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
-
Escherichia coli B
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
21000
-
x * 21000, SDS-PAGE
Escherichia coli B
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
additional information
Escherichia coli B
methionine sulfoxide reduction pathway, overview
?
-
-
-
Organism
Organism
UniProt
Commentary
Textmining
Escherichia coli
-
-
-
Escherichia coli B
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
native enzyme about 1100fold by ammonium sulfate fractionation, anion exchange chromatography, gel filtration, again anion exchange chromatography, heat treatment, and hydroxyapatite chromatography to near homogeneity
Escherichia coli B
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.00452
-
purified enzyme
Escherichia coli B
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
L-methionine (S)-S-oxide + NADPH
-
393068
Escherichia coli B
L-methionine + NADP+ + H2O
-
-
-
?
L-methionine (S)-S-oxide + reduced DTT
highest activity at 10 mM
393068
Escherichia coli B
L-methionine + oxidized DTT + H2O
-
-
-
?
additional information
methionine sulfoxide reduction pathway, overview
393068
Escherichia coli B
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 21000, SDS-PAGE
Escherichia coli B
Synonyms
Synonyms
Commentary
Organism
methionine sulfoxide reductase
-
Escherichia coli
methionine sulfoxide reductase
-
Escherichia coli B
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Escherichia coli B
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Escherichia coli B
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
required, can be substituted by DTT, but not by GSH or 2-mercaptoethanol
Escherichia coli B
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
required, can be substituted by DTT, but not by GSH or 2-mercaptoethanol
Escherichia coli B
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0003
-
L-methionine (S)-S-oxide
pH 7.5, 37C
Escherichia coli B
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
-
Escherichia coli B
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
21000
-
x * 21000, SDS-PAGE
Escherichia coli B
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
additional information
Escherichia coli B
methionine sulfoxide reduction pathway, overview
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
native enzyme about 1100fold by ammonium sulfate fractionation, anion exchange chromatography, gel filtration, again anion exchange chromatography, heat treatment, and hydroxyapatite chromatography to near homogeneity
Escherichia coli B
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.00452
-
purified enzyme
Escherichia coli B
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
L-methionine (S)-S-oxide + NADPH
-
393068
Escherichia coli B
L-methionine + NADP+ + H2O
-
-
-
?
L-methionine (S)-S-oxide + reduced DTT
highest activity at 10 mM
393068
Escherichia coli B
L-methionine + oxidized DTT + H2O
-
-
-
?
additional information
methionine sulfoxide reduction pathway, overview
393068
Escherichia coli B
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 21000, SDS-PAGE
Escherichia coli B
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Escherichia coli B
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Escherichia coli B
Other publictions for EC 1.8.4.13
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
658212
Weissbach
Methionine sulfoxide reductase ...
Escherichia coli
Biochim. Biophys. Acta
1703
203-212
2005
-
1
-
-
-
-
-
-
1
-
-
3
-
1
-
-
-
-
-
-
1
-
9
-
1
-
-
-
-
-
-
-
2
-
-
-
-
1
-
2
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
1
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
393068
Ejiri
The purification of methionine ...
Escherichia coli, Escherichia coli B
Anal. Biochem.
102
393-398
1980
-
-
-
-
-
-
-
1
-
1
1
1
-
2
-
-
1
-
-
-
1
-
3
1
2
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
1
1
-
-
-
1
-
-
1
-
3
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
393071
Ejiri
Reduction of methionine sulfox ...
Escherichia coli K-12 2276, Escherichia coli K-12
J. Bacteriol.
139
161-164
1979
-
-
-
-
-
-
-
-
2
-
-
2
-
2
-
-
1
-
-
-
-
-
4
-
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
2
-
-
-
1
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
393070
Black
-
A specific enzymatic reduction ...
Saccharomyces cerevisiae
J. Biol. Chem.
235
2910-2916
1960
-
-
-
-
-
-
7
1
-
-
-
2
-
1
-
-
1
-
-
-
-
-
4
-
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
7
-
1
-
-
-
2
-
-
-
1
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-