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Literature summary for 1.8.4.12 extracted from

  • Shen, W.; Han, J.; Yan, P.; Zheng, J.; Zhang, L.; Li, X.; Tuo, D.; Zhou, P.
    Soluble expression of biologically active methionine sulfoxide reductase B1 (PaMsrB1) from Carica papaya in Escherichia coli and isolation of its protein targets (2018), Protein Expr. Purif., 146, 17-22 .
    View publication on PubMed
Search for more literature for 1.8.4.12

Cloned(Commentary)

Cloned (Comment) Organism
PaMsrB1 as a fusion protein containing an N-terminal maltose-binding protein (MBP) is expressed in Escherichia coli Rosetta (DE3) cells. Production of soluble fusion protein is greater when the cells are cultured at 16 °C than at 37°C Carica papaya

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
16900
-
 SDS-PAGE Carica papaya

Organism

Organism UniProt Comment Textmining
Carica papaya F2YS22
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Carica papaya

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dabsyl-L-methionine (R)-sulfoxide + dithiothreitol
-
 Carica papaya dabsyl-L-methionine + dithiothreitol disulfide + H2O
-
 ?
L-methionine-(R)-S-oxide + dithiothreitol
-
 Carica papaya L-methionine + dithiothreitol disulfide + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 16900, SDS-PAGE Carica papaya

Synonyms

Synonyms Comment Organism
methionine sulfoxide reductase B1
-
 Carica papaya
PaMsrB1
-
 Carica papaya

General Information

General Information Comment Organism
physiological function methionine sulfoxide reductase B1 (MsrB1) protects the photosynthetic apparatus from oxidative damage by scavenging reactive oxygen species to repair Met-oxidized proteins in response to abiotic stresses and biotic attack Carica papaya