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Literature summary for 1.8.4.12 extracted from

  • Xiao, T.; Mi, M.; Wang, C.; Qian, M.; Chen, Y.; Zheng, L.; Zhang, H.; Hu, Z.; Shen, Z.; Xia, Y.
    A methionine-R-sulfoxide reductase, OsMSRB5, is required for rice defense against copper toxicity (2018), Environ. Exp. Bot., 153, 45-53 .
No PubMed abstract available
Search for more literature for 1.8.4.12

Cloned(Commentary)

Cloned (Comment) Organism
gene OsMSRB5, sequence comparisons and phylogenetic analysis, quantitative real-time PCR enzyme expression analysis, recombinant ectopic expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), recombinant expression of N- or C-terminally sGFP-tagged enzyme in onion epidermal cells Oryza sativa Japonica Group

Protein Variants

Protein Variants Comment Organism
additional information generation of a mutant with osmsrb5 (PFG_3D-00669.R) with T-DNA insertion in the promoter region of the OsMSRB5 gene Oryza sativa Japonica Group

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
 Oryza sativa Japonica Group 5634
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-methionine (R)-S-oxide + thioredoxin Oryza sativa Japonica Group
-
 L-methionine + thioredoxin disulfide + H2O
-
 ?
peptide-L-methionine (R)-S-oxide + thioredoxin Oryza sativa Japonica Group
-
 peptide-L-methionine + thioredoxin disulfide + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Oryza sativa Japonica Group Q10L32 cv. Dongjing
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and ultrafiltration, to homogeneity Oryza sativa Japonica Group

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Oryza sativa Japonica Group
-
additional information OsMSRB5 is mainly expressed in leaves, with low transcriptional levels of OsMSRB5 observed in seeds, stems, and roots Oryza sativa Japonica Group
-
root
-
 Oryza sativa Japonica Group
-
seed
-
 Oryza sativa Japonica Group
-
seedling
-
 Oryza sativa Japonica Group
-
stem very low content Oryza sativa Japonica Group
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-methionine (R)-S-oxide + thioredoxin
-
 Oryza sativa Japonica Group L-methionine + thioredoxin disulfide + H2O
-
 ?
additional information OsMSRB5 has the ability to reduce free methionine-R-sulfoxide (Met-R-SO) and protein-bound-like Met-SO (dabsyl-Met-SO) to Met and dabsyl-Met, respectively Oryza sativa Japonica Group ?
-
-
peptide-L-methionine (R)-S-oxide + thioredoxin
-
 Oryza sativa Japonica Group peptide-L-methionine + thioredoxin disulfide + H2O
-
 ?

Synonyms

Synonyms Comment Organism
methionine sulfoxide reductase
-
 Oryza sativa Japonica Group
More cf. EC 1.8.4.14 Oryza sativa Japonica Group
MSR
-
 Oryza sativa Japonica Group
OsMSRB5
-
 Oryza sativa Japonica Group

Expression

Organism Comment Expression
Oryza sativa Japonica Group OsMSRB5 is induced by the excess copper (Cu) treatment up

General Information

General Information Comment Organism
evolution phylogenetic analysis suggested that OsMSRB5 is a B-type MSR with similar structure to MSRBs of other species, and belongs to 2-Cys MSRB Oryza sativa Japonica Group
additional information the enzyme harbors two CXXC motifs (M1 and M3) including four non-redox-active cysteines, as well as XCGWP (M2) and RXCXNS (M4) conserved domains Oryza sativa Japonica Group
physiological function methionine-R-sulfoxide reductase OsMSRB5 is required for rice defense against copper toxicity Oryza sativa Japonica Group