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Literature summary for 1.8.4.12 extracted from

  • Sasoni, N.; Hartman, M.; Guerrero, S.; Iglesias, A.; Arias, D.
    Functional characterization of methionine sulfoxide reductases from Leptospira interrogans (2021), Biochim. Biophys. Acta Proteins Proteom., 1869, 140575 .
    View publication on PubMed
Search for more literature for 1.8.4.12

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 (DE3) fused to an C-terminal 6Histag Leptospira interrogans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0028
-
 glutaredoxin 30°C, pH 7.0, cosubstrate: N-acetyl-L-methionine-(R)-S-oxide Leptospira interrogans
0.026
-
 thioredoxin 30°C, pH 7.0, cosubstrate: N-acetyl-L-methionine-(R)-S-oxide Leptospira interrogans
0.21
-
 N-acetyl-L-methionine-(R)-S-oxide 30°C, pH 7.0, cosubstrate: glutaredoxin Leptospira interrogans
1
-
 L-methionine-(R)-S-oxide 30°C, pH 7.0, cosubstrate: glutaredoxin Leptospira interrogans

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm dual localization, both in cytoplasm and periplasm Leptospira interrogans 5737
-
periplasm dual localization, both in cytoplasm and periplasm Leptospira interrogans
-
-

Organism

Organism UniProt Comment Textmining
Leptospira interrogans Q72NN2 serovar Copenhageni
-
Leptospira interrogans Fiocruz L1-130 Q72NN2 serovar Copenhageni
-

Purification (Commentary)

Purification (Comment) Organism
-
 Leptospira interrogans

Storage Stability

Storage Stability Organism
-80°C, 8 months, no loss of activity Leptospira interrogans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-methionine-(R)-S-oxide + glutaredoxin glutaredoxin from Homo sapiens Leptospira interrogans L-methionine + glutaredoxin disulfide + H2O
-
 ?
L-methionine-(R)-S-oxide + glutaredoxin glutaredoxin from Homo sapiens Leptospira interrogans Fiocruz L1-130 L-methionine + glutaredoxin disulfide + H2O
-
 ?
L-methionine-(R)-S-oxide + thioredoxin thioredoxin from Leptospira interrogans Leptospira interrogans L-methionine + thioredoxin disulfide + H2O
-
 ?
L-methionine-(R)-S-oxide + thioredoxin thioredoxin from Leptospira interrogans Leptospira interrogans Fiocruz L1-130 L-methionine + thioredoxin disulfide + H2O
-
 ?
N-acetyl-L-methionine-(R)-S-oxide + glutaredoxin glutaredoxin from Homo sapiens Leptospira interrogans N-acetyl-L-methionine + glutaredoxin disulfide + H2O
-
 ?
N-acetyl-L-methionine-(R)-S-oxide + glutaredoxin glutaredoxin from Homo sapiens Leptospira interrogans Fiocruz L1-130 N-acetyl-L-methionine + glutaredoxin disulfide + H2O
-
 ?

Synonyms

Synonyms Comment Organism
MsrB
-
 Leptospira interrogans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0043
-
 thioredoxin 30°C, pH 7.0, cosubstrate: N-acetyl-L-methionine-(R)-S-oxide Leptospira interrogans
0.0076
-
 L-methionine-(R)-S-oxide 30°C, pH 7.0, cosubstrate: glutaredoxin Leptospira interrogans
0.014
-
 N-acetyl-L-methionine-(R)-S-oxide 30°C, pH 7.0, cosubstrate: glutaredoxin Leptospira interrogans
0.0145
-
 glutaredoxin 30°C, pH 7.0, cosubstrate: N-acetyl-L-methionine-(R)-S-oxide Leptospira interrogans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0076
-
 L-methionine-(R)-S-oxide 30°C, pH 7.0, cosubstrate: glutaredoxin Leptospira interrogans
0.067
-
 N-acetyl-L-methionine-(R)-S-oxide 30°C, pH 7.0, cosubstrate: glutaredoxin Leptospira interrogans
0.17
-
 thioredoxin 30°C, pH 7.0, cosubstrate: N-acetyl-L-methionine-(R)-S-oxide Leptospira interrogans
5.2
-
 glutaredoxin 30°C, pH 7.0, cosubstrate: N-acetyl-L-methionine-(R)-S-oxide Leptospira interrogans