Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.8.4.12 extracted from

  • Liu, L.; Wang, M.H.
    Cloning, expression, and characterization of a methionine sulfoxide reductase B gene from Nicotiana tabacum (2013), Protein J., 32, 543-550 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Nicotiana tabacum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
thioredoxin pH 8.0, 37°C Nicotiana tabacum

Localization

Localization Comment Organism GeneOntology No. Textmining
plastid
-
Nicotiana tabacum 9536
-

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dabsyl-L-methionine (R)-S-oxide + dithiothreitol
-
Nicotiana tabacum dabsyl-L-methionine + dithiothreitol disulfide + H2O
-
?
L-methionine (R)-S-oxide + thioredoxin
-
Nicotiana tabacum L-methionine + thioredoxin disulfide + H2O
-
?

Subunits

Subunits Comment Organism
? x * 24000, SDS-PAGE Nicotiana tabacum

Synonyms

Synonyms Comment Organism
MsrB3
-
Nicotiana tabacum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Nicotiana tabacum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
32
-
89% of maximum activity Nicotiana tabacum
42
-
98% of maximum activity Nicotiana tabacum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0000032
-
thioredoxin pH 8.0, 37°C Nicotiana tabacum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Nicotiana tabacum

pH Range

pH Minimum pH Maximum Comment Organism
6
-
3% of maximum activity Nicotiana tabacum

Expression

Organism Comment Expression
Nicotiana tabacum expression is upregulated by abscisic acid, salt, cold, and methyl viologen stress within 24 h of treatment up

General Information

General Information Comment Organism
physiological function Escherichia coli cells harboring MSRB3 display increased viability under H2O2 stress Nicotiana tabacum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0008
-
thioredoxin pH 8.0, 37°C Nicotiana tabacum