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Literature summary for 1.8.4.12 extracted from

  • Tarrago, L.; Laugier, E.; Zaffagnini, M.; Marchand, C.; Le Marechal, P.; Rouhier, N.; Lemaire, S.D.; Rey, P.
    Regeneration mechanisms of Arabidopsis thaliana methionine sulfoxide reductases B by glutaredoxins and thioredoxins (2009), J. Biol. Chem., 284, 18963-18971.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.8.4.12

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli M15rep4 cells Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
C116S MSRB1 mutant with decreased kcat values for dabsyl-L-methionine (R)-sulfoxide compared to the wild type enzyme Arabidopsis thaliana
C134T MSRB2 mutant with increased kcat values for dabsyl-L-methionine (R)-sulfoxide compared to the wild type enzyme Arabidopsis thaliana
T132A MSRB1 mutant with decreased kcat values for dabsyl-L-methionine (R)-sulfoxide compared to the wild type enzyme Arabidopsis thaliana
T132C MSRB1 mutant with decreased kcat values for dabsyl-L-methionine (R)-sulfoxide compared to the wild type enzyme Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0068
-
 glutaredoxin C4 isoform MSRB1 wild type enzyme Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
plastid
-
 Arabidopsis thaliana 9536
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
16610
-
 reduced isoform MSRB1, MALDI-TOF mass spectrometry Arabidopsis thaliana
16620
-
 reduced isoform MSRB1, calculated from amino acid sequence Arabidopsis thaliana
16860
-
 reduced isoform MSRB2, calculated from amino acid sequence Arabidopsis thaliana
16860
-
 reduced isoform MSRB2, MALDI-TOF mass spectrometry Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HiTrap column chromatography and Sephadex G-25 gel filtration Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dabsyl-L-methionine (R)-sulfoxide + glutaredoxin C4
-
 Arabidopsis thaliana ?
-
 ?
dabsyl-L-methionine (R)-sulfoxide + glutaredoxin S12
-
 Arabidopsis thaliana ?
-
 ?
dabsyl-L-methionine (R)-sulfoxide + thioredoxin
-
 Arabidopsis thaliana dabsyl-L-methionine + thioredoxin disulfide + H2O
-
 ?
dabsyl-L-methionine (R)-sulfoxide + thioredoxin h1
-
 Arabidopsis thaliana ?
-
 ?
L-methionine (R)-sulfoxide + thioredoxin
-
 Arabidopsis thaliana L-methionine + thioredoxin disulfide + H2O
-
 ?

Synonyms

Synonyms Comment Organism
methionine sulfoxide reductase B
-
 Arabidopsis thaliana
MSRB1 isoform Arabidopsis thaliana
MsrB2 isoform Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.002
-
 dabsyl-L-methionine (R)-sulfoxide isoform MSRB1 wild type enzyme, in the presence of 20 mMTris(2-carboxyethyl) phosphine hydrochloride Arabidopsis thaliana
0.005
-
 dabsyl-L-methionine (R)-sulfoxide isoform MSRB1 mutant T132C, in the presence of 20 mM dithioerythritol Arabidopsis thaliana
0.012
-
 dabsyl-L-methionine (R)-sulfoxide isoform MSRB1 mutant T132A, in the presence of 20 mM dithioerythritol Arabidopsis thaliana
0.014
-
 dabsyl-L-methionine (R)-sulfoxide isoform MSRB2 wild type enzyme, in the presence of 20 mMTris(2-carboxyethyl) phosphine hydrochloride Arabidopsis thaliana
0.02
-
 glutaredoxin C4 isoform MSRB2 wild type enzyme Arabidopsis thaliana
0.028
-
 dabsyl-L-methionine (R)-sulfoxide isoform MSRB2 wild type enzyme, in the presence of 20 mM dithioerythritol Arabidopsis thaliana
0.03
-
 glutaredoxin S12 isoform MSRB2 wild type enzyme Arabidopsis thaliana
0.047
-
 dabsyl-L-methionine (R)-sulfoxide isoform MSRB1 mutant C116S, in the presence of 20 mM dithioerythritol Arabidopsis thaliana
0.064
-
 dabsyl-L-methionine (R)-sulfoxide isoform MSRB2 mutant C134T, in the presence of 20 mM dithioerythritol Arabidopsis thaliana
0.075
-
 dabsyl-L-methionine (R)-sulfoxide isoform MSRB1 wild type enzyme, in the presence of 20 mM dithioerythritol Arabidopsis thaliana
0.3
-
 glutaredoxin C4 isoform MSRB2 mutant enzyme C134T Arabidopsis thaliana
0.31
-
 glutaredoxin S12 isoform MSRB1 mutant enzyme T132A Arabidopsis thaliana
0.34
-
 glutaredoxin C4 isoform MSRB1 mutant enzyme C116S Arabidopsis thaliana
0.36
-
 glutaredoxin S12 isoform MSRB2 mutant enzyme C134T Arabidopsis thaliana
0.37
-
 glutaredoxin C4 isoform MSRB1 mutant enzyme T132A Arabidopsis thaliana
0.48
-
 glutaredoxin C4 isoform MSRB1 wild type enzyme Arabidopsis thaliana
0.5
-
 glutaredoxin S12 isoform MSRB1 mutant enzyme C116S Arabidopsis thaliana
0.58
-
 glutaredoxin S12 isoform MSRB1 wild type enzyme Arabidopsis thaliana
1.28
-
 thioredoxin h1 isoform MSRB2 wild type enzyme Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
thioredoxin
-
 Arabidopsis thaliana

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
73.4
-
 glutaredoxin C4 isoform MSRB1 wild type enzyme Arabidopsis thaliana