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Literature summary for 1.8.4.12 extracted from

  • Brot, N.; Collet, J.F.; Johnson, L.C.; Jonsson, T.J.; Weissbach, H.; Lowther, W.T.
    The thioredoxin domain of Neisseria gonorrhoeae PilB can use electrons from DsbD to reduce downstream methionine sulfoxide reductases (2006), J. Biol. Chem., 281, 32668-32675.
    View publication on PubMed
Search for more literature for 1.8.4.12

Cloned(Commentary)

Cloned (Comment) Organism
gene pilB, expression of N-terminally His-tagged full-length wild-type and mutant PilB and MsrB domain variants in Escherichia coli Neisseria gonorrhoeae

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant PilB mutant L38M/L41M, vapour diffusion method, 30 mg/ml protein in 20 mM HEPES, pH 7.5, and 100 mM NaCl, is mixed with well solution containing 0.1 M MES, pH 6.5, 0.2 M ammonium sulfate, 26% PEG 2000 monomethylester, and 25% glycerol, X-ray diffraction structure determination and analysis at 1.6 A resolution, multiwavelength anomalous dispersion at -170°C Neisseria gonorrhoeae

Protein Variants

Protein Variants Comment Organism
L38M/L41M site-directed mutagenesis, mutation of the NT domain of PilB, thioredoxin binding structure, crystal structure analysis, overview Neisseria gonorrhoeae

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
 Neisseria gonorrhoeae
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information PilB is a selenocysteine-containing enzyme Neisseria gonorrhoeae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Neisseria gonorrhoeae The thioredoxin domain of PilB can use electrons from DsbD to reduce downstream methionine sulfoxide reductases, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Neisseria gonorrhoeae
-
 bifunctional enzyme MsrA/B, gene pilB
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His-tagged full-length PilB and MsrB domain variants from Escherichia coli by nickel affinity chromatography followed by cleavage of the His-Tag through thrombin, followed by gel filtration and ion exchange chomatography Neisseria gonorrhoeae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information The thioredoxin domain of PilB can use electrons from DsbD to reduce downstream methionine sulfoxide reductases, overview Neisseria gonorrhoeae ?
-
 ?
additional information the bifunctional enzyme catalyzes both reactions of MsrB or PilB, EC 1.8.4.12, and of MsrA or PilA, EC 1.8.4.11, the catalytic sites for the two different activities are localized separately on the enzyme molecule, overview Neisseria gonorrhoeae ?
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 ?

Synonyms

Synonyms Comment Organism
MsrB
-
 Neisseria gonorrhoeae
PilB
-
 Neisseria gonorrhoeae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Neisseria gonorrhoeae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Neisseria gonorrhoeae

Cofactor

Cofactor Comment Organism Structure
thioredoxin PilB contains an N-terminal thioredoxin-like domain, the NT domain, fused to the MsrA and MSrB domains, structure overview Neisseria gonorrhoeae