Literature summary for 1.8.4.12 extracted from

Gustavsson, N.; Kokke, B.P.; Harndahl, U.; Silow, M.; Bechtold, U.; Poghosyan, Z.; Murphy, D.; Boelens, W.C.; Sundby, C.
A peptide methionine sulfoxide reductase highly expressed in photosynthetic tissue in Arabidopsis thaliana can protect the chaperone-like activity of a chloroplast-localized small heat shock protein (2002), Plant J., 29, 545-553.

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Application

Application	Comment	Organism
biotechnology	enzyme is a target for modification of redox-dependent regulation	Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment)	Organism
expression of the plastidic isozyme pPMSR in Escherichia coli strain BL21(DE3) without the chloroplast signal sequence	Arabidopsis thaliana

Protein Variants

Protein Variants	Comment	Organism
C151A	site-directed mutagenesis, activity with Hsp21 is similar to the wild-type enzyme	Arabidopsis thaliana

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
chloroplast	plastidic isozyme pPMSR	Arabidopsis thaliana	9507	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
Hsp21 L-methionine S-oxide + dithiothreitol	Arabidopsis thaliana	chloroplast-localized small heat shock protein, repair function for heat shock protein Hsp21 by restoring the structure, which is crucial for cellular resistance to oxidative stress, the enzyme can protect the chaperone-like activity of Hsp21	Hsp21 L-methionine + dithiothreitol S-oxide	-	?
additional information	Arabidopsis thaliana	enzyme has regulatory function in the plant cell	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	-	5 genes, including 1 plastidic isozyme	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant plastidic isozyme pPMSR from Escherichia coli strain BL21(DE3)	Arabidopsis thaliana

Source Tissue

Source Tissue	Comment	Organism	Textmining
additional information	high expression level of the plastidic isozyme pPMSR in photosynthetic active tissue	Arabidopsis thaliana	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Hsp21 L-methionine S-oxide + dithiothreitol	chloroplast-localized small heat shock protein, repair function for heat shock protein Hsp21 by restoring the structure, which is crucial for cellular resistance to oxidative stress, the enzyme can protect the chaperone-like activity of Hsp21	Arabidopsis thaliana	Hsp21 L-methionine + dithiothreitol S-oxide	-	?
Hsp21 L-methionine S-oxide + dithiothreitol	Hsp21 contains 6 methionine residues at positions 49, 52, 55, 59, 62, and 67, about half of the residues are reduced by the enzyme probably due to its stereospecificity	Arabidopsis thaliana	Hsp21 L-methionine + dithiothreitol S-oxide	-	?
additional information	enzyme has regulatory function in the plant cell	Arabidopsis thaliana	?	-	?

Synonyms

Synonyms	Comment	Organism
peptide methionine sulfoxide reductase	-	Arabidopsis thaliana
PMSR	-	Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Arabidopsis thaliana

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.8	-	assay at	Arabidopsis thaliana

Cofactor

Cofactor	Comment	Organism	Structure
dithiothreitol	absolutely dependent on in vitro and in vivo with substrate Hsp21	Arabidopsis thaliana

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Release 2023.1 (January 2023)