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Literature summary for 1.8.4.12 extracted from
- Methionine sulphoxide reductase is an important antioxidant enzyme in the gastric pathogen Helicobacter pylori (2004), Mol. Microbiol., 53, 1397-1406.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| msr gene, DNA sequence determination and analysis, subcloning in Escherichia coli strain DH5-alpha, functional complementation of the enzyme-deficient mutant with the wild-type gene | Helicobacter pylori |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of an enzyme-deficient mutant strain which shows diminished growth in presence of chemical oxidants with rapid loss of viability compared to the wild-type strain, activity can be recovered by complementation with the wild-type gene, study of oxidative stress resistance and colonization activity | Helicobacter pylori |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Helicobacter pylori | 16020 | - |
| additional information | no activity in the cytoplasm | Helicobacter pylori | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
x * 43000, Msr, SDS-PAGE | Helicobacter pylori |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-methionine (R)-sulfoxide + thioredoxin | Helicobacter pylori | important antioxidant enzyme and colonization factor in the gastric pathogen, a methionine repair enzyme responsible for stress resistance | L-methionine + thioredoxin disulfide + H2O | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Helicobacter pylori | - |
enzyme forms MsrA and MsrB are fused together forming a single protein termed Msr | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-methionine (R)-sulfoxide + thioredoxin | - |
Helicobacter pylori | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| L-methionine (R)-sulfoxide + thioredoxin | important antioxidant enzyme and colonization factor in the gastric pathogen, a methionine repair enzyme responsible for stress resistance | Helicobacter pylori | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| additional information | the enzyme also exhibits MsrA activity utilizing L-methionine (S)-sulfoxide as substrate | Helicobacter pylori | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 43000, Msr, SDS-PAGE | Helicobacter pylori |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| methionine sulphoxide reductase | - |
Helicobacter pylori |
| MSR | - |
Helicobacter pylori |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Helicobacter pylori |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| thioredoxin | - |
Helicobacter pylori | |
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Release 2023.1 (January 2023)
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