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Literature summary for 1.8.4.12 extracted from
- Methionine sulfoxide reductases in prokaryotes (2005), Biochim. Biophys. Acta, 1703, 221-229.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | enzyme is induced under oxidative stress and heat shock | Mycobacterium tuberculosis | |
| additional information | the msrA1-msrB operon is induced by antibiotics | Staphylococcus aureus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| 3 genes msrA and 1 gene msrB form an operon, one of the 3 msrA genes is fused to the msrB gene, genetic organization and regulation, overview | Staphylococcus aureus |
| chromosome 1 contains 2 genes msrB, chromosome 2 contains 1 gene msrB | Vibrio cholerae serotype O1 |
| gene msrB, located in the chromosome at 40.09 min, respectively, regulation mechanism of gene expression, overview | Escherichia coli |
| genes msrA and msrB are translationally fused | Neisseria gonorrhoeae |
| genes msrA, EC 1.8.4.11, and msrB form an operon | Bacillus subtilis |
| genes msrB and msrA, EC 1.8.4.11, are translationally fused | Neisseria meningitidis |
| the chromosome contains 2 copies of gene msrB, a plasmid harbors 1 copy of gene msrB | Sinorhizobium meliloti |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | mutant strains produce a truncated version of fused MsrA/MsrB with increased sensitivity to H2O2 and superoxide anions | Neisseria gonorrhoeae |
| additional information | mutation of the msr genes impair virulence, overview, mutation of the msrA1 operon leads to increased susceptibility to H2O2 | Staphylococcus aureus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | secretion of MsrA, EC 1.8.4.11, and MsrB fused together | Helicobacter pylori | - |
- |
| membrane | outer cell membrane | Neisseria gonorrhoeae | 16020 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-methionine (R)-sulfoxide + thioredoxin | Staphylococcus aureus | MsrB is specific for the R-form | L-methionine + thioredoxin disulfide + H2O | - |
? |  |
| L-methionine (R)-sulfoxide + thioredoxin | Sinorhizobium meliloti | MsrB is specific for the R-form | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| L-methionine (R)-sulfoxide + thioredoxin | Bacillus subtilis | MsrB is specific for the R-form | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| L-methionine (R)-sulfoxide + thioredoxin | Escherichia coli | MsrB is specific for the R-form | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| L-methionine (R)-sulfoxide + thioredoxin | Mycolicibacterium smegmatis | MsrB is specific for the R-form | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| L-methionine (R)-sulfoxide + thioredoxin | Neisseria gonorrhoeae | MsrB is specific for the R-form | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| L-methionine (R)-sulfoxide + thioredoxin | Neisseria meningitidis | MsrB is specific for the R-form | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| L-methionine (R)-sulfoxide + thioredoxin | Mycobacterium tuberculosis | MsrB is specific for the R-form | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| L-methionine (R)-sulfoxide + thioredoxin | Helicobacter pylori | MsrB is specific for the R-form | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| L-methionine (R)-sulfoxide + thioredoxin | Dickeya chrysanthemi | MsrB is specific for the R-form | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| L-methionine (R)-sulfoxide + thioredoxin | Vibrio cholerae serotype O1 | MsrB is specific for the R-form | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| L-methionine (R)-sulfoxide + thioredoxin | Aggregatibacter actinomycetemcomitans | MsrB is specific for the R-form | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| additional information | Neisseria gonorrhoeae | enzyme acts on free and protein-bound methionine | ? | - |
? | |
| additional information | Neisseria meningitidis | enzyme acts on free and protein-bound methionine | ? | - |
? | |
| additional information | Mycobacterium tuberculosis | enzyme repairs oxidatively damaged free and protein bound methionine and recycles it from methionine sulfoxide, e.g. the heat shock protein and chaperone Hsp16.3, role of the MsrA/MsrB repair pathway in cellular protein dynamics | ? | - |
? | |
| additional information | Staphylococcus aureus | enzyme repairs oxidatively damaged free and protein bound methionine and recycles it from methionine sulfoxide, role of the MsrA/MsrB repair pathway in cellular protein dynamics | ? | - |
? | |
| additional information | Sinorhizobium meliloti | enzyme repairs oxidatively damaged free and protein bound methionine and recycles it from methionine sulfoxide, role of the MsrA/MsrB repair pathway in cellular protein dynamics | ? | - |
? | |
| additional information | Bacillus subtilis | enzyme repairs oxidatively damaged free and protein bound methionine and recycles it from methionine sulfoxide, role of the MsrA/MsrB repair pathway in cellular protein dynamics | ? | - |
? | |
| additional information | Mycolicibacterium smegmatis | enzyme repairs oxidatively damaged free and protein bound methionine and recycles it from methionine sulfoxide, role of the MsrA/MsrB repair pathway in cellular protein dynamics | ? | - |
? | |
| additional information | Helicobacter pylori | enzyme repairs oxidatively damaged free and protein bound methionine and recycles it from methionine sulfoxide, role of the MsrA/MsrB repair pathway in cellular protein dynamics | ? | - |
? | |
| additional information | Dickeya chrysanthemi | enzyme repairs oxidatively damaged free and protein bound methionine and recycles it from methionine sulfoxide, role of the MsrA/MsrB repair pathway in cellular protein dynamics | ? | - |
? | |
| additional information | Vibrio cholerae serotype O1 | enzyme repairs oxidatively damaged free and protein bound methionine and recycles it from methionine sulfoxide, role of the MsrA/MsrB repair pathway in cellular protein dynamics | ? | - |
? | |
| additional information | Escherichia coli | enzyme repairs oxidatively damaged free and protein bound methionine and recycles it from methionine sulfoxide, role of the MsrA/MsrB repair pathway in cellular protein dynamics, the MsrA/MsrB repair pathway is involved in the signal recognition particle-dependent protein targeting pathway, regulation mechanism of gene expression, overview | ? | - |
? | |
| additional information | Aggregatibacter actinomycetemcomitans | role of the MsrA/MsrB repair pathway in cellular protein dynamics, enzyme repairs oxidatively damaged free and protein bound methionine and recycles it from methionine sulfoxide | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aggregatibacter actinomycetemcomitans | - |
- |
- |
| Bacillus subtilis | - |
- |
- |
| Dickeya chrysanthemi | - |
- |
- |
| Escherichia coli | - |
1 copy gene msrB | - |
| Helicobacter pylori | - |
- |
- |
| Mycobacterium tuberculosis | - |
- |
- |
| Mycolicibacterium smegmatis | - |
- |
- |
| Neisseria gonorrhoeae | - |
- |
- |
| Neisseria meningitidis | - |
- |
- |
| no activity in Aquifex aeolicus | - |
- |
- |
| no activity in Thermotoga maritima | - |
- |
- |
| Sinorhizobium meliloti | - |
3 copies of gene msrB | - |
| Staphylococcus aureus | - |
1 copy of gene msrB | - |
| Vibrio cholerae serotype O1 | - |
3 isozymes of MsrB | - |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| L-methionine (R)-sulfoxide + thioredoxin = L-methionine + thioredoxin disulfide + H2O | catalytic mechanism involves the formation of a sulfenic acid intermediate | Staphylococcus aureus | |
| L-methionine (R)-sulfoxide + thioredoxin = L-methionine + thioredoxin disulfide + H2O | catalytic mechanism involves the formation of a sulfenic acid intermediate | Sinorhizobium meliloti | |
| L-methionine (R)-sulfoxide + thioredoxin = L-methionine + thioredoxin disulfide + H2O | catalytic mechanism involves the formation of a sulfenic acid intermediate | Bacillus subtilis | |
| L-methionine (R)-sulfoxide + thioredoxin = L-methionine + thioredoxin disulfide + H2O | catalytic mechanism involves the formation of a sulfenic acid intermediate | Escherichia coli | |
| L-methionine (R)-sulfoxide + thioredoxin = L-methionine + thioredoxin disulfide + H2O | catalytic mechanism involves the formation of a sulfenic acid intermediate | Mycolicibacterium smegmatis | |
| L-methionine (R)-sulfoxide + thioredoxin = L-methionine + thioredoxin disulfide + H2O | catalytic mechanism involves the formation of a sulfenic acid intermediate | Neisseria gonorrhoeae | |
| L-methionine (R)-sulfoxide + thioredoxin = L-methionine + thioredoxin disulfide + H2O | catalytic mechanism involves the formation of a sulfenic acid intermediate | Neisseria meningitidis | |
| L-methionine (R)-sulfoxide + thioredoxin = L-methionine + thioredoxin disulfide + H2O | catalytic mechanism involves the formation of a sulfenic acid intermediate | Mycobacterium tuberculosis | |
| L-methionine (R)-sulfoxide + thioredoxin = L-methionine + thioredoxin disulfide + H2O | catalytic mechanism involves the formation of a sulfenic acid intermediate | Helicobacter pylori | |
| L-methionine (R)-sulfoxide + thioredoxin = L-methionine + thioredoxin disulfide + H2O | catalytic mechanism involves the formation of a sulfenic acid intermediate | Dickeya chrysanthemi | |
| L-methionine (R)-sulfoxide + thioredoxin = L-methionine + thioredoxin disulfide + H2O | catalytic mechanism involves the formation of a sulfenic acid intermediate | Vibrio cholerae serotype O1 | |
| L-methionine (R)-sulfoxide + thioredoxin = L-methionine + thioredoxin disulfide + H2O | catalytic mechanism involves the formation of a sulfenic acid intermediate | Aggregatibacter actinomycetemcomitans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-methionine (R)-sulfoxide + thioredoxin | MsrB is specific for the R-form | Staphylococcus aureus | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| L-methionine (R)-sulfoxide + thioredoxin | MsrB is specific for the R-form | Sinorhizobium meliloti | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| L-methionine (R)-sulfoxide + thioredoxin | MsrB is specific for the R-form | Bacillus subtilis | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| L-methionine (R)-sulfoxide + thioredoxin | MsrB is specific for the R-form | Escherichia coli | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| L-methionine (R)-sulfoxide + thioredoxin | MsrB is specific for the R-form | Mycolicibacterium smegmatis | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| L-methionine (R)-sulfoxide + thioredoxin | MsrB is specific for the R-form | Neisseria gonorrhoeae | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| L-methionine (R)-sulfoxide + thioredoxin | MsrB is specific for the R-form | Neisseria meningitidis | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| L-methionine (R)-sulfoxide + thioredoxin | MsrB is specific for the R-form | Mycobacterium tuberculosis | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| L-methionine (R)-sulfoxide + thioredoxin | MsrB is specific for the R-form | Helicobacter pylori | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| L-methionine (R)-sulfoxide + thioredoxin | MsrB is specific for the R-form | Dickeya chrysanthemi | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| L-methionine (R)-sulfoxide + thioredoxin | MsrB is specific for the R-form | Vibrio cholerae serotype O1 | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| L-methionine (R)-sulfoxide + thioredoxin | MsrB is specific for the R-form | Aggregatibacter actinomycetemcomitans | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| additional information | enzyme acts on free and protein-bound methionine | Staphylococcus aureus | ? | - |
? | |
| additional information | enzyme acts on free and protein-bound methionine | Sinorhizobium meliloti | ? | - |
? | |
| additional information | enzyme acts on free and protein-bound methionine | Bacillus subtilis | ? | - |
? | |
| additional information | enzyme acts on free and protein-bound methionine | Escherichia coli | ? | - |
? | |
| additional information | enzyme acts on free and protein-bound methionine | Mycolicibacterium smegmatis | ? | - |
? | |
| additional information | enzyme acts on free and protein-bound methionine | Neisseria gonorrhoeae | ? | - |
? | |
| additional information | enzyme acts on free and protein-bound methionine | Neisseria meningitidis | ? | - |
? | |
| additional information | enzyme acts on free and protein-bound methionine | Mycobacterium tuberculosis | ? | - |
? | |
| additional information | enzyme acts on free and protein-bound methionine | Helicobacter pylori | ? | - |
? | |
| additional information | enzyme acts on free and protein-bound methionine | Dickeya chrysanthemi | ? | - |
? | |
| additional information | enzyme acts on free and protein-bound methionine | Vibrio cholerae serotype O1 | ? | - |
? | |
| additional information | enzyme acts on free and protein-bound methionine | Aggregatibacter actinomycetemcomitans | ? | - |
? | |
| additional information | enzyme repairs oxidatively damaged free and protein bound methionine and recycles it from methionine sulfoxide, e.g. the heat shock protein and chaperone Hsp16.3, role of the MsrA/MsrB repair pathway in cellular protein dynamics | Mycobacterium tuberculosis | ? | - |
? | |
| additional information | enzyme repairs oxidatively damaged free and protein bound methionine and recycles it from methionine sulfoxide, role of the MsrA/MsrB repair pathway in cellular protein dynamics | Staphylococcus aureus | ? | - |
? | |
| additional information | enzyme repairs oxidatively damaged free and protein bound methionine and recycles it from methionine sulfoxide, role of the MsrA/MsrB repair pathway in cellular protein dynamics | Sinorhizobium meliloti | ? | - |
? | |
| additional information | enzyme repairs oxidatively damaged free and protein bound methionine and recycles it from methionine sulfoxide, role of the MsrA/MsrB repair pathway in cellular protein dynamics | Bacillus subtilis | ? | - |
? | |
| additional information | enzyme repairs oxidatively damaged free and protein bound methionine and recycles it from methionine sulfoxide, role of the MsrA/MsrB repair pathway in cellular protein dynamics | Mycolicibacterium smegmatis | ? | - |
? | |
| additional information | enzyme repairs oxidatively damaged free and protein bound methionine and recycles it from methionine sulfoxide, role of the MsrA/MsrB repair pathway in cellular protein dynamics | Helicobacter pylori | ? | - |
? | |
| additional information | enzyme repairs oxidatively damaged free and protein bound methionine and recycles it from methionine sulfoxide, role of the MsrA/MsrB repair pathway in cellular protein dynamics | Dickeya chrysanthemi | ? | - |
? | |
| additional information | enzyme repairs oxidatively damaged free and protein bound methionine and recycles it from methionine sulfoxide, role of the MsrA/MsrB repair pathway in cellular protein dynamics | Vibrio cholerae serotype O1 | ? | - |
? | |
| additional information | enzyme repairs oxidatively damaged free and protein bound methionine and recycles it from methionine sulfoxide, role of the MsrA/MsrB repair pathway in cellular protein dynamics, the MsrA/MsrB repair pathway is involved in the signal recognition particle-dependent protein targeting pathway, regulation mechanism of gene expression, overview | Escherichia coli | ? | - |
? | |
| additional information | role of the MsrA/MsrB repair pathway in cellular protein dynamics, enzyme repairs oxidatively damaged free and protein bound methionine and recycles it from methionine sulfoxide | Aggregatibacter actinomycetemcomitans | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | MsrA, EC 1.8.4.11, and MsrB are fused together | Helicobacter pylori |
| More | MsrB and enzyme MsrA, EC 1.8.4.11, form domains of a single polypeptide together with a third thioredoxin-like domain | Neisseria meningitidis |
| More | the 2 enzyme activities, MsrA and MsrB, form domains of a single polypeptide together with a third thioredoxin-like domain | Neisseria gonorrhoeae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| methionine sulfoxide reductase | - |
Staphylococcus aureus |
| methionine sulfoxide reductase | - |
Sinorhizobium meliloti |
| methionine sulfoxide reductase | - |
Bacillus subtilis |
| methionine sulfoxide reductase | - |
Escherichia coli |
| methionine sulfoxide reductase | - |
Mycolicibacterium smegmatis |
| methionine sulfoxide reductase | - |
Neisseria gonorrhoeae |
| methionine sulfoxide reductase | - |
Neisseria meningitidis |
| methionine sulfoxide reductase | - |
Mycobacterium tuberculosis |
| methionine sulfoxide reductase | - |
Helicobacter pylori |
| methionine sulfoxide reductase | - |
Dickeya chrysanthemi |
| methionine sulfoxide reductase | - |
Vibrio cholerae serotype O1 |
| methionine sulfoxide reductase | - |
Aggregatibacter actinomycetemcomitans |
| MsrB | - |
Staphylococcus aureus |
| MsrB | - |
Sinorhizobium meliloti |
| MsrB | - |
Bacillus subtilis |
| MsrB | - |
Escherichia coli |
| MsrB | - |
Mycolicibacterium smegmatis |
| MsrB | - |
Neisseria gonorrhoeae |
| MsrB | - |
Neisseria meningitidis |
| MsrB | - |
Mycobacterium tuberculosis |
| MsrB | - |
Helicobacter pylori |
| MsrB | - |
Dickeya chrysanthemi |
| MsrB | - |
Vibrio cholerae serotype O1 |
| MsrB | - |
Aggregatibacter actinomycetemcomitans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| thioredoxin | - |
Staphylococcus aureus | |
| thioredoxin | - |
Sinorhizobium meliloti | |
| thioredoxin | - |
Bacillus subtilis | |
| thioredoxin | - |
Escherichia coli | |
| thioredoxin | - |
Mycolicibacterium smegmatis | |
| thioredoxin | - |
Neisseria gonorrhoeae | |
| thioredoxin | - |
Neisseria meningitidis | |
| thioredoxin | - |
Mycobacterium tuberculosis | |
| thioredoxin | - |
Helicobacter pylori | |
| thioredoxin | - |
Dickeya chrysanthemi | |
| thioredoxin | - |
Vibrio cholerae serotype O1 | |
| thioredoxin | - |
Aggregatibacter actinomycetemcomitans | |
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