Cloned (Comment) | Organism |
---|---|
gene selR, DNA and amino acid sequence determination and analysis, expression as His-tagged enzyme in Escherichia coli strain BL21(DE3) | Drosophila melanogaster |
overexpression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
selenium | selenoprotein | Mus musculus | |
selenium | selenoprotein | Drosophila melanogaster | |
Zn2+ | metalloenzyme, content determination | Mus musculus | |
Zn2+ | metalloenzyme, content determination | Drosophila melanogaster |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-methionine (R)-sulfoxide + thioredoxin | Mus musculus | MsrB is absolute specific for the R-form, no activity with the S-form, pathway overview | L-methionine + thioredoxin disulfide + H2O | - |
? | |
L-methionine (R)-sulfoxide + thioredoxin | Drosophila melanogaster | MsrB is absolute specific for the R-form, no activity with the S-form, pathway overview | L-methionine + thioredoxin disulfide + H2O | - |
? | |
additional information | Mus musculus | enzyme provides protection for the cell against oxidative stress | ? | - |
? | |
additional information | Drosophila melanogaster | enzyme provides protection for the cell against oxidative stress | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | Q8INK9 | - |
- |
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) | Drosophila melanogaster |
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by heparin affinity chromatography | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-methionine (R)-sulfoxide + thioredoxin | MsrB is absolute specific for the R-form, no activity with the S-form, pathway overview | Mus musculus | L-methionine + thioredoxin disulfide + H2O | - |
? | |
L-methionine (R)-sulfoxide + thioredoxin | MsrB is absolute specific for the R-form, no activity with the S-form, pathway overview | Drosophila melanogaster | L-methionine + thioredoxin disulfide + H2O | - |
? | |
L-methionine (R)-sulfoxide + thioredoxin | MsrB is absolute specific for the R-form, no activity with the S-form | Mus musculus | L-methionine + thioredoxin disulfide + H2O | - |
? | |
L-methionine (R)-sulfoxide + thioredoxin | MsrB is absolute specific for the R-form, no activity with the S-form | Drosophila melanogaster | L-methionine + thioredoxin disulfide + H2O | - |
? | |
additional information | enzyme provides protection for the cell against oxidative stress | Mus musculus | ? | - |
? | |
additional information | enzyme provides protection for the cell against oxidative stress | Drosophila melanogaster | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
methionine-R-sulfoxide reductase | - |
Mus musculus |
methionine-R-sulfoxide reductase | - |
Drosophila melanogaster |
selenoprotein R | - |
Mus musculus |
selenoprotein R | - |
Drosophila melanogaster |
SelR | - |
Mus musculus |
SelR | - |
Drosophila melanogaster |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thioredoxin | - |
Mus musculus | |
thioredoxin | - |
Drosophila melanogaster |