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Literature summary for 1.8.4.11 extracted from

  • Sasoni, N.; Hartman, M.; Guerrero, S.; Iglesias, A.; Arias, D.
    Functional characterization of methionine sulfoxide reductases from Leptospira interrogans (2021), Biochim. Biophys. Acta Proteins Proteom., 1869, 140575 .
    View publication on PubMed
Search for more literature for 1.8.4.11

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 (DE3) fused to a 6His-MBP Leptospira interrogans
expression in Escherichia coli BL21 (DE3) fused to an N-terminal 6Histag Leptospira interrogans

Protein Variants

Protein Variants Comment Organism
C18S exhibits lower enzymatic activity than the wild type enzyme Leptospira interrogans
C25S exhibits lower enzymatic activity than the wild type enzyme Leptospira interrogans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0018
-
 thioredoxin 30°C, pH 7.0, cosubstrate: N-acetyl-L-methionine-(S)-S-oxide, wild-type enzyme Leptospira interrogans
0.002
-
 thioredoxin 30°C, pH 7.0, cosubstrate: L-methionine-(S)-S-oxide, wild-type enzyme Leptospira interrogans
0.019
-
 thioredoxin 30°C, pH 7.0, cosubstrate: N-acetyl-L-methionine-(S)-S-oxide, wild-type enzyme Leptospira interrogans
0.0249
-
 N-acetyl-L-methionine-(S)-S-oxide 30°C, pH 7.0, cosubstrate: thioredoxin, wild-type enzyme Leptospira interrogans
0.0305
-
 thioredoxin 30°C, pH 7.0, cosubstrate: L-methionine-(S)-S-oxide, wild-type enzyme Leptospira interrogans
0.135
-
 L-methionine-(S)-S-oxide 30°C, pH 7.0, cosubstrate: thioredoxin, mutant enzyme C25S Leptospira interrogans
0.28
-
 N-acetyl-L-methionine-(S)-S-oxide 30°C, pH 7.0, cosubstrate: thioredoxin, mutant enzyme C25S Leptospira interrogans
0.39
-
 L-methionine-(S)-S-oxide 30°C, pH 7.0, cosubstrate: thioredoxin, wild-type enzyme Leptospira interrogans
0.55
-
 L-methionine-(S)-S-oxide 30°C, pH 7.0, cosubstrate: thioredoxin, wild-type enzyme Leptospira interrogans
1.4
-
 N-acetyl-L-methionine-(S)-S-oxide 30°C, pH 7.0, cosubstrate: thioredoxin, wild-type enzyme Leptospira interrogans

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm dual localization, both in cytoplasm and periplasm Leptospira interrogans 5737
-
periplasm dual localization, both in cytoplasm and periplasm Leptospira interrogans
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21000
-
 calculated from sequence Leptospira interrogans
23000
-
 calculated from sequence Leptospira interrogans

Organism

Organism UniProt Comment Textmining
Leptospira interrogans Q72N60 serovar Copenhageni
-
Leptospira interrogans Q72UW2 serovar Copenhageni
-
Leptospira interrogans Fiocruz L1-130 Q72N60 serovar Copenhageni
-
Leptospira interrogans Fiocruz L1-130 Q72UW2 serovar Copenhageni
-

Purification (Commentary)

Purification (Comment) Organism
-
 Leptospira interrogans

Storage Stability

Storage Stability Organism
-80°C, 8 months, no loss of activity Leptospira interrogans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-methionine-(S)-S-oxide + thioredoxin thioredoxin from Leptospira interrogans Leptospira interrogans L-methionine + thioredoxin disulfide + H2O
-
 ?
L-methionine-(S)-S-oxide + thioredoxin thioredoxin from Leptospira interrogans Leptospira interrogans Fiocruz L1-130 L-methionine + thioredoxin disulfide + H2O
-
 ?
N-acetyl-L-methionine-(S)-S-oxide + thioredoxin thioredoxin from Leptospira interrogans Leptospira interrogans N-acetyl-L-methionine + thioredoxin disulfide + H2O
-
 ?
N-acetyl-L-methionine-(S)-S-oxide + thioredoxin thioredoxin from Leptospira interrogans Leptospira interrogans Fiocruz L1-130 N-acetyl-L-methionine + thioredoxin disulfide + H2O
-
 ?

Synonyms

Synonyms Comment Organism
LIC_10545
-
 Leptospira interrogans
LIC_12978
-
 Leptospira interrogans
linmsra1
-
 Leptospira interrogans
linmsra2
-
 Leptospira interrogans
MsrA1
-
 Leptospira interrogans
MSRA2
-
 Leptospira interrogans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0054
-
 L-methionine-(S)-S-oxide 30°C, pH 7.0, cosubstrate: thioredoxin, mutant enzyme C25S Leptospira interrogans
0.0548
-
 N-acetyl-L-methionine-(S)-S-oxide 30°C, pH 7.0, cosubstrate: thioredoxin, mutant enzyme C25S Leptospira interrogans
0.083
-
 thioredoxin 30°C, pH 7.0, cosubstrate: L-methionine-(S)-S-oxide, wild-type enzyme Leptospira interrogans
0.098
-
 L-methionine-(S)-S-oxide 30°C, pH 7.0, cosubstrate: thioredoxin, wild-type enzyme Leptospira interrogans
0.102
-
 thioredoxin 30°C, pH 7.0, cosubstrate: N-acetyl-L-methionine-(S)-S-oxide, wild-type enzyme Leptospira interrogans
0.203
-
 N-acetyl-L-methionine-(S)-S-oxide 30°C, pH 7.0, cosubstrate: thioredoxin, wild-type enzyme Leptospira interrogans
0.32
-
 thioredoxin 30°C, pH 7.0, cosubstrate: N-acetyl-L-methionine-(S)-S-oxide, wild-type enzyme Leptospira interrogans
0.39
-
 L-methionine-(S)-S-oxide 30°C, pH 7.0, cosubstrate: thioredoxin, wild-type enzyme Leptospira interrogans
0.415
-
 N-acetyl-L-methionine-(S)-S-oxide 30°C, pH 7.0, cosubstrate: thioredoxin, wild-type enzyme Leptospira interrogans
0.508
-
 thioredoxin 30°C, pH 7.0, cosubstrate: L-methionine-(S)-S-oxide, wild-type enzyme Leptospira interrogans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.02
-
 N-acetyl-L-methionine-(S)-S-oxide 30°C, pH 7.0, cosubstrate: thioredoxin, mutant enzyme C25S Leptospira interrogans
0.04
-
 L-methionine-(S)-S-oxide 30°C, pH 7.0, cosubstrate: thioredoxin, mutant enzyme C25S Leptospira interrogans
0.14
-
 N-acetyl-L-methionine-(S)-S-oxide 30°C, pH 7.0, cosubstrate: thioredoxin, wild-type enzyme Leptospira interrogans
0.15
-
 N-acetyl-L-methionine-(S)-S-oxide 30°C, pH 7.0, cosubstrate: thioredoxin, wild-type enzyme Leptospira interrogans
0.25
-
 L-methionine-(S)-S-oxide 30°C, pH 7.0, cosubstrate: thioredoxin, wild-type enzyme Leptospira interrogans
0.71
-
 L-methionine-(S)-S-oxide 30°C, pH 7.0, cosubstrate: thioredoxin, wild-type enzyme Leptospira interrogans
40
-
 thioredoxin 30°C, pH 7.0, cosubstrate: N-acetyl-L-methionine-(S)-S-oxide, wild-type enzyme Leptospira interrogans
42
-
 thioredoxin 30°C, pH 7.0, cosubstrate: L-methionine-(S)-S-oxide, wild-type enzyme Leptospira interrogans
50
-
 thioredoxin 30°C, pH 7.0, cosubstrate: L-methionine-(S)-S-oxide, wild-type enzyme Leptospira interrogans
56
-
 thioredoxin 30°C, pH 7.0, cosubstrate: N-acetyl-L-methionine-(S)-S-oxide, wild-type enzyme Leptospira interrogans