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Literature summary for 1.8.4.11 extracted from

  • Kim, S.; Lee, K.; Park, S.H.; Kwak, G.H.; Kim, M.S.; Kim, H.Y.; Hwang, K.Y.
    Structural insights into a bifunctional peptide methionine sulfoxide reductase MsrA/B fusion protein from Helicobacter pylori (2021), Antioxidants (Basel), 10, 389 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.8.4.11

Cloned(Commentary)

Cloned (Comment) Organism
expresion in Escherichia coli BL-21 Star (DE3) Helicobacter pylori

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, crystal structure of HpMsrAB C44S/C318S at 2.2 A, which shows that a linker region (Hpiloop, 193-205) between two domains interacts with each HpMsrA or HpMsrB domain via three salt bridges (E193-K107, D197-R103, and K200-D339) Helicobacter pylori

Protein Variants

Protein Variants Comment Organism
D197A kcat/Km for dabsyl-L-methionine (S)-sulfoxide is 1.46fold lower than wild-type value Helicobacter pylori
E193A kcat/Km for dabsyl-L-methionine (S)-sulfoxide is 1.3fold lower than wild-type value Helicobacter pylori
E193A/D197A kcat/Km for dabsyl-L-methionine (S)-sulfoxide is 1.3fold lower than wild-type value Helicobacter pylori
E339A kcat/Km for dabsyl-L-methionine (S)-sulfoxide is 1.5fold higher than wild-type value Helicobacter pylori
Y343F kcat/Km for dabsyl-L-methionine (S)-sulfoxide is 1.25fold lower than wild-type value Helicobacter pylori

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 dabsyl-L-methionine (S)-sulfoxide pH 7.5, 37°C, mutant enzyme E339A Helicobacter pylori
0.12
-
 dabsyl-L-methionine (S)-sulfoxide pH 7.5, 37°C, mutant enzyme E193A/D197A Helicobacter pylori
0.12
-
 dabsyl-L-methionine (S)-sulfoxide pH 7.5, 37°C, mutant enzyme Y343F Helicobacter pylori
0.14
-
 dabsyl-L-methionine (S)-sulfoxide pH 7.5, 37°C, mutant enzyme E193A Helicobacter pylori
0.15
-
 dabsyl-L-methionine (S)-sulfoxide pH 7.5, 37°C, mutant enzyme D197A Helicobacter pylori
0.17
-
 dabsyl-L-methionine (S)-sulfoxide pH 7.5, 37°C, wild-type enzyme Helicobacter pylori

Organism

Organism UniProt Comment Textmining
Helicobacter pylori O25011
-
-
Helicobacter pylori ATCC 700392 O25011
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Helicobacter pylori

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dabsyl-L-methionine (S)-sulfoxide + dithiothreitol
-
 Helicobacter pylori dabsyl-L-methionine + dithiothreitol disulfide + H2O
-
 ?
dabsyl-L-methionine (S)-sulfoxide + dithiothreitol
-
 Helicobacter pylori ATCC 700392 dabsyl-L-methionine + dithiothreitol disulfide + H2O
-
 ?

Synonyms

Synonyms Comment Organism
msrAB
-
 Helicobacter pylori

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.058
-
 dabsyl-L-methionine (S)-sulfoxide pH 7.5, 37°C, mutant enzyme E339A Helicobacter pylori
0.095
-
 dabsyl-L-methionine (S)-sulfoxide pH 7.5, 37°C, mutant enzyme E193A/D197A Helicobacter pylori
0.097
-
 dabsyl-L-methionine (S)-sulfoxide pH 7.5, 37°C, mutant enzyme Y343F Helicobacter pylori
0.1
-
 dabsyl-L-methionine (S)-sulfoxide pH 7.5, 37°C, mutant enzyme D197A Helicobacter pylori
0.108
-
 dabsyl-L-methionine (S)-sulfoxide pH 7.5, 37°C, mutant enzyme E193A Helicobacter pylori
0.17
-
 dabsyl-L-methionine (S)-sulfoxide pH 7.5, 37°C, wild-type enzyme Helicobacter pylori

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.68
-
 dabsyl-L-methionine (S)-sulfoxide pH 7.5, 37°C, mutant enzyme D197A Helicobacter pylori
0.77
-
 dabsyl-L-methionine (S)-sulfoxide pH 7.5, 37°C, mutant enzyme E193A Helicobacter pylori
0.78
-
 dabsyl-L-methionine (S)-sulfoxide pH 7.5, 37°C, mutant enzyme E193A/D197A Helicobacter pylori
0.8
-
 dabsyl-L-methionine (S)-sulfoxide pH 7.5, 37°C, mutant enzyme Y343F Helicobacter pylori
1
-
 dabsyl-L-methionine (S)-sulfoxide pH 7.5, 37°C, wild-type enzyme Helicobacter pylori
1.47
-
 dabsyl-L-methionine (S)-sulfoxide pH 7.5, 37°C, mutant enzyme E339A Helicobacter pylori