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Literature summary for 1.8.4.11 extracted from

  • Arias, D.G.; Cabeza, M.S.; Erben, E.D.; Carranza, P.G.; Lujan, H.D.; Tellez Inon, M.T.; Iglesias, A.A.; Guerrero, S.A.
    Functional characterization of methionine sulfoxide reductase A from Trypanosoma spp. (2010), Free Radic. Biol. Med., 50, 37-46.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Trypanosoma cruzi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.013
-
tryparedoxin I recombinant enzyme. isoform MSRA, at 30°C and pH 7.5 Trypanosoma cruzi
0.033
-
tryparedoxin I recombinant enzyme. isoform MSR10, at 30°C and pH 7.5 Trypanosoma cruzi
0.069
-
L-methionine (S)-sulfoxide recombinant enzyme. isoform MSRA, at 30°C and pH 7.5 Trypanosoma cruzi
0.723
-
L-methionine (S)-sulfoxide recombinant enzyme. isoform MSR10, at 30°C and pH 7.5 Trypanosoma cruzi

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Trypanosoma cruzi 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
deduced from amino acid sequence Trypanosoma cruzi
23000
-
His-tagged enzyme isoform MSRA, SDS-PAGE Trypanosoma cruzi
24000
-
His-tagged enzyme isoform MSR10, SDS-PAGE Trypanosoma cruzi
30000
-
His-tagged enzyme isoform MSR180, SDS-PAGE Trypanosoma cruzi

Organism

Organism UniProt Comment Textmining
Trypanosoma cruzi
-
-
-
Trypanosoma cruzi CL Brener
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Co2+-IDA-Sepharose column chromatography Trypanosoma cruzi

Source Tissue

Source Tissue Comment Organism Textmining
epimastigote
-
Trypanosoma cruzi
-

Storage Stability

Storage Stability Organism
-80°C, 20 mM Tris-HCl pH 7.5, 1 mM EDTA, and 4% (v/v) glycerol, at least 12 months, the purified recombinant enzyme remains stable Trypanosoma cruzi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-methionine (S)-sulfoxide + tryparedoxin I L-methionine (S)-sulfoxide is the specific substrate Trypanosoma cruzi L-methionine + tryparedoxin I disulfide + H2O
-
?
L-methionine (S)-sulfoxide + tryparedoxin I L-methionine (S)-sulfoxide is the specific substrate Trypanosoma cruzi CL Brener L-methionine + tryparedoxin I disulfide + H2O
-
?

Subunits

Subunits Comment Organism
monomer 1 * 20000, deduced from amino acid sequence Trypanosoma cruzi

Synonyms

Synonyms Comment Organism
methionine sulfoxide reductase A
-
Trypanosoma cruzi
MSR10 isoform Trypanosoma cruzi
MSR180 isoform Trypanosoma cruzi
MsrA isoform Trypanosoma cruzi

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.32
-
L-methionine (S)-sulfoxide recombinant enzyme. isoform MSRA, at 30°C and pH 7.5 Trypanosoma cruzi
0.32
-
tryparedoxin I recombinant enzyme. isoform MSRA, at 30°C and pH 7.5 Trypanosoma cruzi
0.52
-
L-methionine (S)-sulfoxide recombinant enzyme. isoform MSR10, at 30°C and pH 7.5 Trypanosoma cruzi
0.52
-
tryparedoxin I recombinant enzyme. isoform MSR10, at 30°C and pH 7.5 Trypanosoma cruzi

Cofactor

Cofactor Comment Organism Structure
thioredoxin
-
Trypanosoma cruzi

General Information

General Information Comment Organism
physiological function overexpression of isoform MSR10 in Trypanosoma cruzi confers resistance to oxidative stress, as transfected cells exhibit around 2fold more tolerance to exogenous H2O2 Trypanosoma cruzi

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0086
-
L-methionine (S)-sulfoxide recombinant enzyme. isoform MSR180, at 30°C and pH 7.5 Trypanosoma cruzi
4.6
-
L-methionine (S)-sulfoxide recombinant enzyme. isoform MSRA, at 30°C and pH 7.5 Trypanosoma cruzi
7.2
-
L-methionine (S)-sulfoxide recombinant enzyme. isoform MSR10, at 30°C and pH 7.5 Trypanosoma cruzi
24
-
tryparedoxin I recombinant enzyme. isoform MSRA, at 30°C and pH 7.5 Trypanosoma cruzi
77
-
tryparedoxin I recombinant enzyme. isoform MSR180, at 30°C and pH 7.5 Trypanosoma cruzi
180
-
tryparedoxin I recombinant enzyme. isoform MSR10, at 30°C and pH 7.5 Trypanosoma cruzi