Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Trypanosoma cruzi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
tryparedoxin I | recombinant enzyme. isoform MSRA, at 30°C and pH 7.5 | Trypanosoma cruzi | |
0.033 | - |
tryparedoxin I | recombinant enzyme. isoform MSR10, at 30°C and pH 7.5 | Trypanosoma cruzi | |
0.069 | - |
L-methionine (S)-sulfoxide | recombinant enzyme. isoform MSRA, at 30°C and pH 7.5 | Trypanosoma cruzi | |
0.723 | - |
L-methionine (S)-sulfoxide | recombinant enzyme. isoform MSR10, at 30°C and pH 7.5 | Trypanosoma cruzi |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Trypanosoma cruzi | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20000 | - |
deduced from amino acid sequence | Trypanosoma cruzi |
23000 | - |
His-tagged enzyme isoform MSRA, SDS-PAGE | Trypanosoma cruzi |
24000 | - |
His-tagged enzyme isoform MSR10, SDS-PAGE | Trypanosoma cruzi |
30000 | - |
His-tagged enzyme isoform MSR180, SDS-PAGE | Trypanosoma cruzi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma cruzi | - |
- |
- |
Trypanosoma cruzi CL Brener | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Co2+-IDA-Sepharose column chromatography | Trypanosoma cruzi |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
epimastigote | - |
Trypanosoma cruzi | - |
Storage Stability | Organism |
---|---|
-80°C, 20 mM Tris-HCl pH 7.5, 1 mM EDTA, and 4% (v/v) glycerol, at least 12 months, the purified recombinant enzyme remains stable | Trypanosoma cruzi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-methionine (S)-sulfoxide + tryparedoxin I | L-methionine (S)-sulfoxide is the specific substrate | Trypanosoma cruzi | L-methionine + tryparedoxin I disulfide + H2O | - |
? | |
L-methionine (S)-sulfoxide + tryparedoxin I | L-methionine (S)-sulfoxide is the specific substrate | Trypanosoma cruzi CL Brener | L-methionine + tryparedoxin I disulfide + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 20000, deduced from amino acid sequence | Trypanosoma cruzi |
Synonyms | Comment | Organism |
---|---|---|
methionine sulfoxide reductase A | - |
Trypanosoma cruzi |
MSR10 | isoform | Trypanosoma cruzi |
MSR180 | isoform | Trypanosoma cruzi |
MsrA | isoform | Trypanosoma cruzi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.32 | - |
L-methionine (S)-sulfoxide | recombinant enzyme. isoform MSRA, at 30°C and pH 7.5 | Trypanosoma cruzi | |
0.32 | - |
tryparedoxin I | recombinant enzyme. isoform MSRA, at 30°C and pH 7.5 | Trypanosoma cruzi | |
0.52 | - |
L-methionine (S)-sulfoxide | recombinant enzyme. isoform MSR10, at 30°C and pH 7.5 | Trypanosoma cruzi | |
0.52 | - |
tryparedoxin I | recombinant enzyme. isoform MSR10, at 30°C and pH 7.5 | Trypanosoma cruzi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thioredoxin | - |
Trypanosoma cruzi |
General Information | Comment | Organism |
---|---|---|
physiological function | overexpression of isoform MSR10 in Trypanosoma cruzi confers resistance to oxidative stress, as transfected cells exhibit around 2fold more tolerance to exogenous H2O2 | Trypanosoma cruzi |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0086 | - |
L-methionine (S)-sulfoxide | recombinant enzyme. isoform MSR180, at 30°C and pH 7.5 | Trypanosoma cruzi | |
4.6 | - |
L-methionine (S)-sulfoxide | recombinant enzyme. isoform MSRA, at 30°C and pH 7.5 | Trypanosoma cruzi | |
7.2 | - |
L-methionine (S)-sulfoxide | recombinant enzyme. isoform MSR10, at 30°C and pH 7.5 | Trypanosoma cruzi | |
24 | - |
tryparedoxin I | recombinant enzyme. isoform MSRA, at 30°C and pH 7.5 | Trypanosoma cruzi | |
77 | - |
tryparedoxin I | recombinant enzyme. isoform MSR180, at 30°C and pH 7.5 | Trypanosoma cruzi | |
180 | - |
tryparedoxin I | recombinant enzyme. isoform MSR10, at 30°C and pH 7.5 | Trypanosoma cruzi |