Cloned (Comment) | Organism |
---|---|
gene msrA, DNA and amino acid sequence determination and analysis, expression of His6-tagged MsrA in Escherichia coli strain BL21 | Dickeya chrysanthemi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Dickeya chrysanthemi | MsrA is a virulence determinant for the plant pathogen required for full virulence | ? | - |
? | |
peptide-L-methionine-(S)-S-oxide + thioredoxin | Dickeya chrysanthemi | stereospecific reduction of protein-bound methionine (S)-sulfoxide residues, the enzyme is involved in oxidized protein repair | peptide-L-methionine + thioredoxin disulfide + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dickeya chrysanthemi | - |
gene msrA, wild-type strain 3937 or RH7006 or pin14 | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged MsrA from Escherichia coli strain BL21 by nickel affinity chromatography to homogeneity | Dickeya chrysanthemi |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
virulence on chicory leaves of wild-type and recombinant strains, overview | Dickeya chrysanthemi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | MsrA is a virulence determinant for the plant pathogen required for full virulence | Dickeya chrysanthemi | ? | - |
? | |
peptide-L-methionine-(S)-S-oxide + thioredoxin | stereospecific reduction of protein-bound methionine (S)-sulfoxide residues, the enzyme is involved in oxidized protein repair | Dickeya chrysanthemi | peptide-L-methionine + thioredoxin disulfide + H2O | - |
? | |
peptide-L-methionine-(S)-S-oxide + thioredoxin | stereospecific reduction of protein-bound methionine (S)-sulfoxide residues | Dickeya chrysanthemi | peptide-L-methionine + thioredoxin disulfide + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MsrA | - |
Dickeya chrysanthemi |
peptide methionine sulfoxide reductase | - |
Dickeya chrysanthemi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Dickeya chrysanthemi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Dickeya chrysanthemi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thioredoxin | - |
Dickeya chrysanthemi |