Cloned (Comment) | Organism |
---|---|
overexpression of the full length tandem enzyme, the MsrA, and the MsrB domains, all His-tagged, in Escherichia coli | Neisseria gonorrhoeae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neisseria gonorrhoeae | P14930 | posesses MsrA and MsrB domains; PilB enzyme has 2 catalytic domains showing MsrA, methionine S-oxide reductase (S-form oxidizing), and MsrB, methionine S-oxide reductase (R-form oxidizing), activity, respectively | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged full length tandem enzyme, MsrA, and MsrB domains from Escherichia coli, tags are removed by thrombin digestion | Neisseria gonorrhoeae |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-methionine (S)-sulfoxide + thioredoxin = L-methionine + thioredoxin disulfide + H2O | reaction mechanism, modeling of substrate binding at the active site, Cys72 is involved | Neisseria gonorrhoeae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.083 | - |
purified recombinant MsrA/MsrB tandem domain, substrate L-methionine (S)-sulfoxide | Neisseria gonorrhoeae |
0.15 | - |
purified recombinant MsrA domain alone, substrate L-methionine (S)-sulfoxide | Neisseria gonorrhoeae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-methionine (S)-sulfoxide + thioredoxin | MsrA activity of the tandem domains of PilB, the MsrA domain alone does very poorly utilize the R-isomer | Neisseria gonorrhoeae | L-methionine + thioredoxin disulfide + H2O | - |
? | |
additional information | the tandem domains of PilB also posesses MsrB activity utilizing L-methionine (R)-sulfoxide as substrate, the MsrB domain alone does not utilize the S-isomer | Neisseria gonorrhoeae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | enzyme domain and active site structure, MsrA domain comprises residues 181-362, overview | Neisseria gonorrhoeae |
Synonyms | Comment | Organism |
---|---|---|
methionine sulfoxide reductase | - |
Neisseria gonorrhoeae |
MsrA | - |
Neisseria gonorrhoeae |
PilB | - |
Neisseria gonorrhoeae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Neisseria gonorrhoeae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.9 | - |
assay at | Neisseria gonorrhoeae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thioredoxin | - |
Neisseria gonorrhoeae |