Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a MsrA/MsrB double mutant | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | membrane-associated isozyme, MsrA | Escherichia coli | 16020 | - |
soluble | soluble isozyme MsrA1 | Escherichia coli | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
protein-L-methionine (S)-sulfoxide + thioredoxin | Escherichia coli | MsrA and the soluble isozyme MsrA1 are specific for the S-form, the membrane-associated isozyme reduces both R- and S-stereoisomers of methionine sulfoxide, N-acetylmethionine sulfoxide, and D-Ala-Met-enkephalin | protein-L-methionine + thioredoxin disulfide + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
wild-type strain MC1061, isozyme MsrA, a membrane-associated isozyme, and a soluble isozyme MsrA1 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0004 | - |
membrane vesicles, substrate N-acetyl-L-methionine-(R)-sulfoxide | Escherichia coli |
0.00044 | - |
MsrA, substrate N-acetyl-L-methionine-(S)-sulfoxide | Escherichia coli |
0.00047 | - |
membrane vesicles, substrate N-acetyl-L-methionine-(S)-sulfoxide | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-methionine (R)-sulfoxide + thioredoxin | the membrane-associated isozyme reduces both R- and S-stereoisomers of methionine sulfoxide in proteins | Escherichia coli | L-methionine + thioredoxin disulfide + H2O | - |
? | |
L-methionine (S)-sulfoxide + thioredoxin | MsrA and soluble isozyme MsrA1 are specific for the S-form, the membrane-associated isozyme reduces both R- and S-stereoisomers of methionine sulfoxide in proteins | Escherichia coli | L-methionine + thioredoxin disulfide + H2O | - |
? | |
additional information | the enzymes utilize free and protein-bound L-methionine and N-acetyl-L-methionine as substrates | Escherichia coli | ? | - |
? | |
N-acetyl-L-methionine (R)-sulfoxide + thioredoxin | the membrane-associated isozyme reduces both R- and S-stereoisomer of methionine sulfoxide in proteins | Escherichia coli | N-acetyl-L-methionine + thioredoxin disulfide + H2O | - |
? | |
N-acetyl-L-methionine (S)-sulfoxide + thioredoxin | MsrA and soluble isozyme MsrA1 are specific for the S-form, the membrane-associated isozyme reduces both R- and S-stereoisomers | Escherichia coli | N-acetyl-L-methionine + thioredoxin disulfide + H2O | - |
? | |
protein-L-methionine (S)-sulfoxide + thioredoxin | MsrA and the soluble isozyme MsrA1 are specific for the S-form, the membrane-associated isozyme reduces both R- and S-stereoisomers of methionine sulfoxide, N-acetylmethionine sulfoxide, and D-Ala-Met-enkephalin | Escherichia coli | protein-L-methionine + thioredoxin disulfide + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MsrA | - |
Escherichia coli |
peptide methionine sulfoxide reductase | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | MsrA can also utilize DTT as reductant, the membrane-isozyme shows only poor activity, while MsrA1 is not active with DTT | Escherichia coli | |
thioredoxin | - |
Escherichia coli |