Literature summary for 1.8.4.11 extracted from

Lee, B.C.; Lee, Y.K.; Lee, H.J.; Stadtman, E.R.; Lee, K.H.; Chung, N.
Cloning and characterization of antioxidant enzyme methionine sulfoxide-S-reductase from Caenorhabditis elegans (2005), Arch. Biochem. Biophys., 434, 275-281.

Search for more literature for 1.8.4.11

Cloned(Commentary)

Cloned (Comment)	Organism
gene msrA, expression in Escherichia coli strain BL21(DE3) as N-terminally His-tagged enzyme, the HIs-tag does not influence enzyme activity	Caenorhabditis elegans

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.18	-	dabsyl L-methionine-(S)-sulfoxide	pH 7.4, 37°C, recombinant enzyme	Caenorhabditis elegans

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
29700	-	x * 29700, recombinant MsrA, SDS-PAGE	Caenorhabditis elegans

Organism

Organism	UniProt	Comment	Textmining
Caenorhabditis elegans	O02089	gene msrA	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged MsrA from Escherichia coli strain BL21(DE3) by nickel affinity and ion exchange chromatography, 425fold	Caenorhabditis elegans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.96	-	purified enzyme, substrate dabsyl L-methionine-(S)-sulfoxide with dithiothreitol	Caenorhabditis elegans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
dabsyl-L-methionine (S)-sulfoxide + dithiothreitol	synthetic substrate, MsrA is absolutely specific for the S-form	Caenorhabditis elegans	dabsyl-L-methionine + dithiothreitol disulfide + H2O	-	?
dabsyl-L-methionine (S)-sulfoxide + NADPH + H+	synthetic substrate, MsrA is absolutely specific for the S-form, 7fold lower activity with NADPH compared to DTT	Caenorhabditis elegans	dabsyl-L-methionine + NADP+ + H2O	-	?
dabsyl-L-methionine (S)-sulfoxide + thioredoxin	synthetic substrate, MsrA is absolutely specific for the S-form	Caenorhabditis elegans	dabsyl-L-methionine + thioredoxin disulfide + H2O	-	?
additional information	enzyme converts free and protein-bound methionine	Caenorhabditis elegans	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 29700, recombinant MsrA, SDS-PAGE	Caenorhabditis elegans

Synonyms

Synonyms	Comment	Organism
methionine sulfoxide-S-reductase	-	Caenorhabditis elegans
MsrA	-	Caenorhabditis elegans

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	about	Caenorhabditis elegans

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
17	57	activity rapidly decreases below 17°C and above 57°C	Caenorhabditis elegans

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.06	-	dabsyl L-methionine-(S)-sulfoxide	pH 7.4, 37°C, recombinant enzyme	Caenorhabditis elegans

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	7.5	-	Caenorhabditis elegans

pH Range

pH Minimum	pH Maximum	Comment	Organism
6.5	8.5	sharp decrease below pH 6.5 and above pH 8.5	Caenorhabditis elegans

Cofactor

Cofactor	Comment	Organism	Structure
dithiothreitol	preferred cofactor in vitro	Caenorhabditis elegans
NADPH	-	Caenorhabditis elegans
thioredoxin	-	Caenorhabditis elegans

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Release 2023.1 (January 2023)