Activating Compound | Comment | Organism | Structure |
---|---|---|---|
KCl | enzyme prefers high ionic strength, activation | Saccharomyces cerevisiae | |
Na2SO4 | enzyme prefers high ionic strength, activation | Saccharomyces cerevisiae | |
NaCl | enzyme prefers high ionic strength, activation | Saccharomyces cerevisiae | |
NaF | enzyme prefers high ionic strength, activation | Saccharomyces cerevisiae |
Cloned (Comment) | Organism |
---|---|
expression of His-tagged wild-type and mutant enzymes in Escherichia coli | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
C25S | site-directed mutagenesis, inactive mutant | Saccharomyces cerevisiae |
F26A | site-directed mutagenesis, inactive mutant | Saccharomyces cerevisiae |
F26H | site-directed mutagenesis, inactive mutant | Saccharomyces cerevisiae |
G24A | site-directed mutagenesis, 60% reduced activity compared to the wild-type enzyme | Saccharomyces cerevisiae |
G28A | site-directed mutagenesis, 81% reduced activity compared to the wild-type enzyme | Saccharomyces cerevisiae |
W27A | site-directed mutagenesis, inactive mutant | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.044 | - |
L-methionine (S)-sulfoxide | recombinant wild-type enzyme, pH 7.5, 37°C | Saccharomyces cerevisiae | |
0.12 | - |
L-methionine (S)-sulfoxide | recombinant wild-type enzyme, pH 7.5, 37°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | enzyme prefers high ionic strength, activation | Escherichia coli | |
Na2SO4 | enzyme prefers high ionic strength, activation | Escherichia coli | |
NaCl | enzyme prefers high ionic strength, activation | Escherichia coli | |
NaF | enzyme prefers high ionic strength, activation | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-methionine (S)-sulfoxide + thioredoxin | Escherichia coli | FMsr is absolutely specific for the S-isomer of free methionine sulfoxide, no activity with protein bound methionine sulfoxide | L-methionine + thioredoxin disulfide + H2O | - |
? | |
L-methionine (S)-sulfoxide + thioredoxin | Saccharomyces cerevisiae | MsrA is specific for the S-isomer | L-methionine + thioredoxin disulfide + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partially | Escherichia coli |
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Escherichia coli |
additional information | - |
- |
Saccharomyces cerevisiae |
4.2 | - |
purified recombinant wild-type enzyme, substrate L-methionine (S)-sulfoxide | Saccharomyces cerevisiae |
43 | - |
purified recombinant wild-type enzyme, substrate dabsyl-L-methionine (S)-sulfoxide | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-methyl 4-tolyl sulfoxide + thioredoxin | - |
Saccharomyces cerevisiae | ? | - |
? | |
(S)-methyl 4-tolyl sulfoxide + thioredoxin | FMsr is specific for the S-isomer | Escherichia coli | ? | - |
? | |
dabsyl-L-methionine (S)-sulfoxide + thioredoxin | MsrA is specific for the S-isomer | Saccharomyces cerevisiae | dabsyl-L-methionine + thioredoxin disulfide + H2O | - |
? | |
dabsyl-L-methionine (S)-sulfoxide + thioredoxin | FMsr is specific for the S-isomer | Escherichia coli | dabsyl-L-methionine + thioredoxin disulfide + H2O | - |
? | |
L-methionine (S)-sulfoxide + thioredoxin | FMsr is absolutely specific for the S-isomer of free methionine sulfoxide, no activity with protein bound methionine sulfoxide | Escherichia coli | L-methionine + thioredoxin disulfide + H2O | - |
? | |
L-methionine (S)-sulfoxide + thioredoxin | MsrA is specific for the S-isomer | Saccharomyces cerevisiae | L-methionine + thioredoxin disulfide + H2O | - |
? | |
additional information | substrate specificity | Escherichia coli | ? | - |
? | |
additional information | substrate specificity | Saccharomyces cerevisiae | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FMsr | - |
Escherichia coli |
methionine sulfoxide reductase | - |
Escherichia coli |
methionine sulfoxide reductase | - |
Saccharomyces cerevisiae |
MsrA | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
37 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Escherichia coli |
7.5 | - |
assay at | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thioredoxin | - |
Escherichia coli | |
thioredoxin | - |
Saccharomyces cerevisiae |