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Literature summary for 1.8.3.7 extracted from

  • Grove, T.L.; Lee, K.H.; St Clair, J.; Krebs, C.; Booker, S.J.
    In vitro characterization of AtsB, a radical SAM formylglycine-generating enzyme that contains three [4Fe-4S] clusters (2008), Biochemistry, 47, 7523-7538 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.8.3.7

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
a [sulfatase]-L-cysteine + O2 + a thiol Klebsiella pneumoniae
-
 a [sulfatase]-3-oxo-L-alanine + hydrogen sulfide + a disulfide + H2O
-
 ?
a [sulfatase]-L-cysteine + O2 + a thiol Klebsiella pneumoniae ATCC 700721D
-
 a [sulfatase]-3-oxo-L-alanine + hydrogen sulfide + a disulfide + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae
-
-
-
Klebsiella pneumoniae ATCC 700721D
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, Talon metal affinity resin column chromatography, and Sephadex G-25 gel filtration Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
a [sulfatase]-L-cysteine + O2 + a thiol
-
 Klebsiella pneumoniae a [sulfatase]-3-oxo-L-alanine + hydrogen sulfide + a disulfide + H2O
-
 ?
a [sulfatase]-L-cysteine + O2 + a thiol
-
 Klebsiella pneumoniae ATCC 700721D a [sulfatase]-3-oxo-L-alanine + hydrogen sulfide + a disulfide + H2O
-
 ?
acetyl-Tyr-Tyr-Thr-Ser-Pro-Met-3-oxo-Ala-Ala-Pro-Ala-Arg-Ser-Met-Leu-Leu-Thr-Gly-Asn-COOH + O2 + dithionite
-
 Klebsiella pneumoniae ?
-
 ?
acetyl-Tyr-Tyr-Thr-Ser-Pro-Met-3-oxo-Ala-Ala-Pro-Ala-Arg-Ser-Met-Leu-Leu-Thr-Gly-Asn-COOH + O2 + dithionite
-
 Klebsiella pneumoniae ATCC 700721D ?
-
 ?
additional information no activity with NH2-Tyr-Tyr-Thr-Ser-Pro-Met-Ala-Ala-Pro-Ala-Arg-Ser-Met-Leu-Leu-Thr-Gly-Asn-COOH Klebsiella pneumoniae ?
-
 ?
additional information no activity with NH2-Tyr-Tyr-Thr-Ser-Pro-Met-Ala-Ala-Pro-Ala-Arg-Ser-Met-Leu-Leu-Thr-Gly-Asn-COOH Klebsiella pneumoniae ATCC 700721D ?
-
 ?
NH2-Tyr-Tyr-Thr-Ser-Pro-Met-3-oxo-Ala-Ala-Pro-Ala-Arg-Ser-Met-Leu-Leu-Thr-Gly-Asn-COOH + O2 + dithionite
-
 Klebsiella pneumoniae ?
-
 ?
NH2-Tyr-Tyr-Thr-Ser-Pro-Met-3-oxo-Ala-Ala-Pro-Ala-Arg-Ser-Met-Leu-Leu-Thr-Gly-Asn-COOH + O2 + dithionite
-
 Klebsiella pneumoniae ATCC 700721D ?
-
 ?
NH2-Tyr-Tyr-Thr-Ser-Pro-Met-Cys-Ala-Pro-Ala-Arg-Ser-Met-Leu-Leu-Thr-Gly-Asn-COOH + O2 + dithionite
-
 Klebsiella pneumoniae ?
-
 ?
NH2-Tyr-Tyr-Thr-Ser-Pro-Met-Cys-Ala-Pro-Ala-Arg-Ser-Met-Leu-Leu-Thr-Gly-Asn-COOH + O2 + dithionite
-
 Klebsiella pneumoniae ATCC 700721D ?
-
 ?

Subunits

Subunits Comment Organism
? x * 44237, calculated from amino acid sequence Klebsiella pneumoniae

Synonyms

Synonyms Comment Organism
AtsB
-
 Klebsiella pneumoniae
FGE
-
 Klebsiella pneumoniae

Cofactor

Cofactor Comment Organism Structure
[4Fe-4S]-center the enzyme contains three [4Fe-4S] clusters Klebsiella pneumoniae