Cloned (Comment) | Organism |
---|---|
- |
Mycolicibacterium smegmatis |
- |
Thermomonospora curvata |
Crystallization (Comment) | Organism |
---|---|
- |
Thermomonospora curvata |
Protein Variants | Comment | Organism |
---|---|---|
C187A/C231A/C284S/C298A | ten-fold increased activity compared to wild-type | Thermomonospora curvata |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu+ | CuSO4 and CuCl are almost equally efficient | Mycolicibacterium smegmatis | |
Cu+ | CuSO4 and CuCl are almost equally efficient | Thermomonospora curvata | |
Cu2+ | CuSO4 and CuCl are almost equally efficient | Mycolicibacterium smegmatis | |
Cu2+ | CuSO4 and CuCl are almost equally efficient | Thermomonospora curvata |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycolicibacterium smegmatis | - |
- |
- |
Mycolicibacterium smegmatis DSM 43465 | - |
- |
- |
Thermomonospora curvata | - |
- |
- |
Thermomonospora curvata DSM 43183 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Abz-SALCSPTRA-NH2 + O2 + reduced dithiothreitol | - |
Mycolicibacterium smegmatis | Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 + H2O + hydrogen sulfide + oxidized dithiothreitol | - |
? | |
Abz-SALCSPTRA-NH2 + O2 + reduced dithiothreitol | - |
Thermomonospora curvata | Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 + H2O + hydrogen sulfide + oxidized dithiothreitol | - |
? | |
Abz-SALCSPTRA-NH2 + O2 + reduced dithiothreitol | - |
Mycolicibacterium smegmatis DSM 43465 | Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 + H2O + hydrogen sulfide + oxidized dithiothreitol | - |
? | |
Abz-SALCSPTRA-NH2 + O2 + reduced dithiothreitol | - |
Thermomonospora curvata DSM 43183 | Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 + H2O + hydrogen sulfide + oxidized dithiothreitol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FGE | - |
Mycolicibacterium smegmatis |
FGE | - |
Thermomonospora curvata |
Temperature Stability Minimum [掳C] | Temperature Stability Maximum [掳C] | Comment | Organism |
---|---|---|---|
43 | - |
mutant enzyme C187A/C231A/C284S/C298A, stable up to | Thermomonospora curvata |
51 | - |
wild-type enzyme, stable up to | Thermomonospora curvata |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
Abz-SALCSPTRA-NH2 | mutant enzyme C187A/C231A/C284S/C298A, 25掳C, 5 mM DTT, 50 mM EDTA, 50 mM NaCl, and 50 mM Tris路HCl, pH 8.0 | Thermomonospora curvata | |
0.027 | - |
Abz-SALCSPTRA-NH2 | wild-type enzyme, 25掳C, 5 mM DTT, 50 mM EDTA, 50 mM NaCl, and 50 mM Tris路HCl, 0.002 mM CuSO4, pH 8.0 | Thermomonospora curvata | |
0.07 | - |
Abz-SALCSPTRA-NH2 | mutant enzyme C187A/C231A/C284S/C298A, 25掳C, 5 mM DTT, 50 mM EDTA, 50 mM NaCl, and 50 mM Tris路HCl, 0.002 mM CuSO4, pH 8.0 | Thermomonospora curvata |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.7 | - |
Abz-SALCSPTRA-NH2 | wild-type enzyme, 25掳C, 5 mM DTT, 50 mM EDTA, 50 mM NaCl, and 50 mM Tris路HCl, pH 8.0 | Thermomonospora curvata | |
17 | - |
Abz-SALCSPTRA-NH2 | mutant enzyme C187A/C231A/C284S/C298A, 25掳C, 5 mM DTT, 50 mM EDTA, 50 mM NaCl, and 50 mM Tris路HCl, pH 8.0 | Thermomonospora curvata | |
48.3 | - |
Abz-SALCSPTRA-NH2 | wild-type enzyme, 25掳C, 5 mM DTT, 50 mM EDTA, 50 mM NaCl, and 50 mM Tris路HCl, 0.002 mM CuSO4, pH 8.0 | Thermomonospora curvata | |
333.3 | - |
Abz-SALCSPTRA-NH2 | mutant enzyme C187A/C231A/C284S/C298A, 25掳C, 5 mM DTT, 50 mM EDTA, 50 mM NaCl, and 50 mM Tris路HCl, 0.002 mM CuSO4, pH 8.0 | Thermomonospora curvata |