BRENDA - Enzyme Database show
show all sequences of 1.8.3.5

Arabidopsis thaliana plants possess a specific farnesylcysteine lyase that is involved in detoxification and recycling of farnesylcysteine

Crowell, D.N.; Huizinga, D.H.; Deem, A.K.; Trobaugh, C.; Denton, R.; Sen, S.E.; Plant J. 50, 839-847 (2007)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene FCLY, encoded on chromosome 5, DNAS and amino acid sequence determination and analysis
Arabidopsis thaliana
Engineering
Amino acid exchange
Commentary
Organism
additional information
T-DNA insertions mutants fcly-1 and fcly-2 plants exhibit reduced FCLY mRNA levels, as determined by semi-quantitative RT-PCR, and reduced FC lyase activity compared with Columbia (Col-0) and wild-type siblings. Significantly, germination of fcly-1 and fcly-2 seeds is inhibited by exogenous ABA at concentrations that did not affect wild-type Col-0 seeds
Arabidopsis thaliana
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Arabidopsis thaliana
16020
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-(2E,6E)-farnesyl-L-cysteine + O2 + H2O
Arabidopsis thaliana
the enzyme exhibits specificity for farnesyl-L-cysteine over geranylgeranyl-L-cysteine
(2E,6E)-farnesal + L-cysteine + H2O2
-
-
?
S-(2E,6E)-farnesyl-L-cysteine + O2 + H2O
Arabidopsis thaliana Col-0
the enzyme exhibits specificity for farnesyl-L-cysteine over geranylgeranyl-L-cysteine
(2E,6E)-farnesal + L-cysteine + H2O2
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
P57681
gene FCLY
-
Arabidopsis thaliana Col-0
P57681
gene FCLY
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
additional information
the enzyme is ubiquitously expressed in Arabidopsis tissues and organs
Arabidopsis thaliana
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
N-acetyl-S-(2E,6E)-farnesyl-L-cysteine + O2 + H2O
the enzyme exhibits specificity for farnesyl-L-cysteine over geranylgeranyl-L-cysteine
710279
Arabidopsis thaliana
(2E,6E)-farnesal + N-acetyl-L-cysteine + H2O2
-
-
-
?
N-acetyl-S-(2E,6E)-farnesyl-L-cysteine + O2 + H2O
the enzyme exhibits specificity for farnesyl-L-cysteine over geranylgeranyl-L-cysteine
710279
Arabidopsis thaliana Col-0
(2E,6E)-farnesal + N-acetyl-L-cysteine + H2O2
-
-
-
?
S-(2E,6E)-farnesyl-L-cysteine + O2 + H2O
the enzyme exhibits specificity for farnesyl-L-cysteine over geranylgeranyl-L-cysteine
710279
Arabidopsis thaliana
(2E,6E)-farnesal + L-cysteine + H2O2
-
-
-
?
S-(2E,6E)-farnesyl-L-cysteine + O2 + H2O
the enzyme exhibits specificity for farnesyl-L-cysteine over geranylgeranyl-L-cysteine
710279
Arabidopsis thaliana Col-0
(2E,6E)-farnesal + L-cysteine + H2O2
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Arabidopsis thaliana
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
Commentary
Organism
gene FCLY, encoded on chromosome 5, DNAS and amino acid sequence determination and analysis
Arabidopsis thaliana
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
T-DNA insertions mutants fcly-1 and fcly-2 plants exhibit reduced FCLY mRNA levels, as determined by semi-quantitative RT-PCR, and reduced FC lyase activity compared with Columbia (Col-0) and wild-type siblings. Significantly, germination of fcly-1 and fcly-2 seeds is inhibited by exogenous ABA at concentrations that did not affect wild-type Col-0 seeds
Arabidopsis thaliana
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Arabidopsis thaliana
16020
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-(2E,6E)-farnesyl-L-cysteine + O2 + H2O
Arabidopsis thaliana
the enzyme exhibits specificity for farnesyl-L-cysteine over geranylgeranyl-L-cysteine
(2E,6E)-farnesal + L-cysteine + H2O2
-
-
?
S-(2E,6E)-farnesyl-L-cysteine + O2 + H2O
Arabidopsis thaliana Col-0
the enzyme exhibits specificity for farnesyl-L-cysteine over geranylgeranyl-L-cysteine
(2E,6E)-farnesal + L-cysteine + H2O2
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
additional information
the enzyme is ubiquitously expressed in Arabidopsis tissues and organs
Arabidopsis thaliana
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
N-acetyl-S-(2E,6E)-farnesyl-L-cysteine + O2 + H2O
the enzyme exhibits specificity for farnesyl-L-cysteine over geranylgeranyl-L-cysteine
710279
Arabidopsis thaliana
(2E,6E)-farnesal + N-acetyl-L-cysteine + H2O2
-
-
-
?
N-acetyl-S-(2E,6E)-farnesyl-L-cysteine + O2 + H2O
the enzyme exhibits specificity for farnesyl-L-cysteine over geranylgeranyl-L-cysteine
710279
Arabidopsis thaliana Col-0
(2E,6E)-farnesal + N-acetyl-L-cysteine + H2O2
-
-
-
?
S-(2E,6E)-farnesyl-L-cysteine + O2 + H2O
the enzyme exhibits specificity for farnesyl-L-cysteine over geranylgeranyl-L-cysteine
710279
Arabidopsis thaliana
(2E,6E)-farnesal + L-cysteine + H2O2
-
-
-
?
S-(2E,6E)-farnesyl-L-cysteine + O2 + H2O
the enzyme exhibits specificity for farnesyl-L-cysteine over geranylgeranyl-L-cysteine
710279
Arabidopsis thaliana Col-0
(2E,6E)-farnesal + L-cysteine + H2O2
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Arabidopsis thaliana
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Arabidopsis thaliana
General Information
General Information
Commentary
Organism
malfunction
T-DNA insertions into the FCLY gene cause significant decreases in FC lyase activity and an enhanced response to abscisic acid in seed germination assays. The effects of FCLY mutations on abscisic acidsensitivity are even greater in the presence of exogenous S-(2E,6E)-farnesyl-L-cysteine
Arabidopsis thaliana
metabolism
the enzyme is involved in the salvage cycle for S-(2E,6E)-farnesyl diphosphate in plants, overview
Arabidopsis thaliana
physiological function
Arabidopsis FC lyase recognizes amide-linked S-(2E,6E)-farnesyl-L-cysteine and may have a role in deprenylation of farnesylated proteins
Arabidopsis thaliana
General Information (protein specific)
General Information
Commentary
Organism
malfunction
T-DNA insertions into the FCLY gene cause significant decreases in FC lyase activity and an enhanced response to abscisic acid in seed germination assays. The effects of FCLY mutations on abscisic acidsensitivity are even greater in the presence of exogenous S-(2E,6E)-farnesyl-L-cysteine
Arabidopsis thaliana
metabolism
the enzyme is involved in the salvage cycle for S-(2E,6E)-farnesyl diphosphate in plants, overview
Arabidopsis thaliana
physiological function
Arabidopsis FC lyase recognizes amide-linked S-(2E,6E)-farnesyl-L-cysteine and may have a role in deprenylation of farnesylated proteins
Arabidopsis thaliana
Other publictions for EC 1.8.3.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743865
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Arabidopsis thaliana
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5
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5
6
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1
1
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710279
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Arabidopsis thaliana plants po ...
Arabidopsis thaliana, Arabidopsis thaliana Col-0
Plant J.
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839-847
2007
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5
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