BRENDA - Enzyme Database
show all sequences of 1.8.3.5

Stereospecificity and kinetic mechanism of human prenylcysteine lyase, an unusual thioether oxidase

Digits, J.A.; Pyun, H.J.; Coates, R.M.; Casey, P.J.; J. Biol. Chem. 277, 41086-41093 (2002)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
farnesal
non-competitive versus farnesyl-L-cysteine
Homo sapiens
farnesol
non-competitive versus farnesyl-L-cysteine
Homo sapiens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.003
-
farnesyl-L-cysteine
-
Homo sapiens
0.05
-
O2
Km below
Homo sapiens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
S-prenyl-L-cysteine + O2 + H2O
Homo sapiens
kinetic mechanism, enzyme transfers the pro-S hydride of the farnesylcysteine to FAD
prenal + L-cysteine + H2O2
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Homo sapiens
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
farnesyl-L-cysteine + O2 + H2O
-
437709
Homo sapiens
farnesal + L-cysteine + H2O2
-
437709
Homo sapiens
?
S-prenyl-L-cysteine + O2 + H2O
-
437709
Homo sapiens
prenal + L-cysteine + H2O2
-
437709
Homo sapiens
?
S-prenyl-L-cysteine + O2 + H2O
kinetic mechanism, enzyme transfers the pro-S hydride of the farnesylcysteine to FAD
437709
Homo sapiens
prenal + L-cysteine + H2O2
-
-
-
?
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
assay at
Homo sapiens
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.000133
-
farnesyl-L-cysteine
-
Homo sapiens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
7.7
assay at, depending on type of assay
Homo sapiens
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
-
Homo sapiens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
-
Homo sapiens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
farnesal
non-competitive versus farnesyl-L-cysteine
Homo sapiens
farnesol
non-competitive versus farnesyl-L-cysteine
Homo sapiens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.003
-
farnesyl-L-cysteine
-
Homo sapiens
0.05
-
O2
Km below
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
S-prenyl-L-cysteine + O2 + H2O
Homo sapiens
kinetic mechanism, enzyme transfers the pro-S hydride of the farnesylcysteine to FAD
prenal + L-cysteine + H2O2
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
farnesyl-L-cysteine + O2 + H2O
-
437709
Homo sapiens
farnesal + L-cysteine + H2O2
-
437709
Homo sapiens
?
S-prenyl-L-cysteine + O2 + H2O
-
437709
Homo sapiens
prenal + L-cysteine + H2O2
-
437709
Homo sapiens
?
S-prenyl-L-cysteine + O2 + H2O
kinetic mechanism, enzyme transfers the pro-S hydride of the farnesylcysteine to FAD
437709
Homo sapiens
prenal + L-cysteine + H2O2
-
-
-
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
assay at
Homo sapiens
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.000133
-
farnesyl-L-cysteine
-
Homo sapiens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
7.7
assay at, depending on type of assay
Homo sapiens
Other publictions for EC 1.8.3.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743865
Dashty
Proteome of human plasma very ...
Homo sapiens
Thromb. Haemost.
111
518-530
2014
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
1
-
-
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
710018
Huizinga
Farnesylcysteine lyase is invo ...
Arabidopsis thaliana
Mol. Plant
3
143-155
2010
-
-
1
-
1
-
6
1
2
-
3
1
-
2
-
1
-
1
-
-
-
-
2
1
3
1
-
-
-
1
-
-
1
-
-
5
-
-
1
1
-
1
-
5
6
-
1
2
-
3
1
-
-
1
-
-
-
-
-
2
1
1
-
-
-
1
-
-
-
-
2
2
-
-
-
706670
Banfi
Proteomic analysis of human lo ...
Homo sapiens
Proteomics
9
1344-1352
2009
-
-
-
-
-
-
1
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
1
-
2
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
710279
Crowell
Arabidopsis thaliana plants po ...
Arabidopsis thaliana, Arabidopsis thaliana Col-0
Plant J.
50
839-847
2007
-
-
1
-
1
-
-
-
1
-
-
2
-
5
-
-
-
-
-
1
-
-
4
-
2
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
4
-
1
-
-
-
1
-
-
-
-
3
3
-
-
-
667445
Wouters
Downregulation of two novel ge ...
Mus musculus
Biochem. Biophys. Res. Commun.
346
491-500
2006
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
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-
-
-
-
-
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-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
669763
Lu
Thematic review series: lipid ...
Bos taurus
J. Lipid Res.
47
1352-1357
2006
1
-
-
-
-
-
-
2
1
-
1
-
-
1
-
-
1
-
-
1
-
-
3
-
3
-
-
-
-
-
-
-
-
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-
1
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-
-
-
-
-
-
-
-
2
1
-
1
-
-
-
-
1
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
437708
Beigneux
Prenylcysteine lyase deficienc ...
Mus musculus
J. Biol. Chem.
277
38358-38363
2002
-
-
1
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
6
-
-
4
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
6
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
437709
Digits
Stereospecificity and kinetic ...
Homo sapiens
J. Biol. Chem.
277
41086-41093
2002
-
-
-
-
-
-
2
2
-
-
-
1
-
2
-
-
-
-
-
-
-
-
3
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1
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1
1
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1
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1
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2
-
2
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1
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3
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1
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1
1
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