BRENDA - Enzyme Database
show all sequences of 1.8.3.4

Purification and properties of methyl mercaptan oxidase from Thiobacillus thioparus TK-m

Gould, W.D.; Kanagawa, T.; J. Gen. Microbiol. 138, 217-221 (1992)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Cu2+
-
Thiobacillus thioparus
CuSO4
-
Thiobacillus thioparus
cyanide
-
Thiobacillus thioparus
ethyl mercaptan
-
Thiobacillus thioparus
methanethiol
-
Thiobacillus thioparus
NEM
-
Thiobacillus thioparus
Sulfide
no inhibition
Thiobacillus thioparus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0313
-
methanethiol
-
Thiobacillus thioparus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
29000
-
gel filtration
Thiobacillus thioparus
40000
-
1 * 40000, SDS-PAGE
Thiobacillus thioparus
Organism
Organism
UniProt
Commentary
Textmining
Thiobacillus thioparus
-
-
-
Thiobacillus thioparus TK-m
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Thiobacillus thioparus
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.27
-
-
Thiobacillus thioparus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
ethyl mercaptan + O2 + H2O
-
395102
Thiobacillus thioparus
acetaldehyde + H2S + H2O2
-
-
-
?
ethyl mercaptan + O2 + H2O
-
395102
Thiobacillus thioparus TK-m
acetaldehyde + H2S + H2O2
-
-
-
?
methanethiol + O2 + H2O
-
395102
Thiobacillus thioparus
formaldehyde + H2S + H2O2
-
395102
Thiobacillus thioparus
?
methanethiol + O2 + H2O
-
395102
Thiobacillus thioparus TK-m
formaldehyde + H2S + H2O2
-
395102
Thiobacillus thioparus TK-m
?
sulfide + O2
no activity
395102
Thiobacillus thioparus
sulfur + H2O2
-
-
-
-
sulfide + O2
no activity
395102
Thiobacillus thioparus TK-m
sulfur + H2O2
-
-
-
-
Subunits
Subunits
Commentary
Organism
monomer
1 * 40000, SDS-PAGE
Thiobacillus thioparus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
9.6
-
Thiobacillus thioparus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Cu2+
-
Thiobacillus thioparus
CuSO4
-
Thiobacillus thioparus
cyanide
-
Thiobacillus thioparus
ethyl mercaptan
-
Thiobacillus thioparus
methanethiol
-
Thiobacillus thioparus
NEM
-
Thiobacillus thioparus
Sulfide
no inhibition
Thiobacillus thioparus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0313
-
methanethiol
-
Thiobacillus thioparus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
29000
-
gel filtration
Thiobacillus thioparus
40000
-
1 * 40000, SDS-PAGE
Thiobacillus thioparus
Purification (Commentary) (protein specific)
Commentary
Organism
-
Thiobacillus thioparus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.27
-
-
Thiobacillus thioparus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
ethyl mercaptan + O2 + H2O
-
395102
Thiobacillus thioparus
acetaldehyde + H2S + H2O2
-
-
-
?
ethyl mercaptan + O2 + H2O
-
395102
Thiobacillus thioparus TK-m
acetaldehyde + H2S + H2O2
-
-
-
?
methanethiol + O2 + H2O
-
395102
Thiobacillus thioparus
formaldehyde + H2S + H2O2
-
395102
Thiobacillus thioparus
?
methanethiol + O2 + H2O
-
395102
Thiobacillus thioparus TK-m
formaldehyde + H2S + H2O2
-
395102
Thiobacillus thioparus TK-m
?
sulfide + O2
no activity
395102
Thiobacillus thioparus
sulfur + H2O2
-
-
-
-
sulfide + O2
no activity
395102
Thiobacillus thioparus TK-m
sulfur + H2O2
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 40000, SDS-PAGE
Thiobacillus thioparus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
9.6
-
Thiobacillus thioparus
Other publictions for EC 1.8.3.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
395104
Kim
-
Isolation and purification of ...
Rhodococcus rhodochrous
Biotechnol. Bioprocess Eng.
5
465-468
2000
-
-
-
-
-
-
3
-
-
-
1
1
-
1
-
-
1
-
-
-
1
-
2
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
1
1
-
-
-
1
-
-
1
-
2
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
395102
Gould
-
Purification and properties of ...
Thiobacillus thioparus, Thiobacillus thioparus TK-m
J. Gen. Microbiol.
138
217-221
1992
-
-
-
-
-
-
7
1
-
-
2
-
-
2
-
-
1
-
-
-
1
-
6
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
1
-
-
2
-
-
-
-
1
-
-
1
-
6
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
395103
Smith
-
Mechanism of oxidation of dime ...
Thiobacillus thioparus, Thiobacillus thioparus E6
J. Gen. Microbiol.
134
3031-3039
1988
-
-
-
-
-
-
1
1
-
-
-
2
-
3
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
1
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
395101
Suylen
-
Methyl mercaptan oxidase, a ke ...
Hyphomicrobium sp., Hyphomicrobium sp. EG
J. Gen. Microbiol.
133
2989-2997
1987
-
-
-
-
-
-
8
3
-
-
2
-
-
2
-
-
1
-
-
-
1
1
6
1
-
-
-
2
-
1
-
-
-
5
-
-
-
-
-
-
-
-
-
-
8
5
3
-
-
2
-
-
-
-
1
-
-
1
1
6
1
-
-
2
-
1
-
-
-
-
-
-
-
-
-