BRENDA - Enzyme Database
show all sequences of 1.8.3.4

Methyl mercaptan oxidase, a key enzyme in the metabolism of methylated sulphur compounds by Hyphomicrobium EG

Suylen, G.M.H.; Large, P.J.; van Dijken, J.P.; Kuenen, J.G.; J. Gen. Microbiol. 133, 2989-2997 (1987)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
(NH4)2SO4
-
Hyphomicrobium sp.
cyanide
-
Hyphomicrobium sp.
ethyl mercaptan
-
Hyphomicrobium sp.
methanethiol
above 0.014 mM
Hyphomicrobium sp.
methylamine
-
Hyphomicrobium sp.
NH4+
-
Hyphomicrobium sp.
NH4Cl
-
Hyphomicrobium sp.
Sulfide
non-competitive inhibition of methyl mercaptan oxidation
Hyphomicrobium sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.005
0.01
methanethiol
-
Hyphomicrobium sp.
0.018
-
ethyl mercaptan
-
Hyphomicrobium sp.
0.06
-
Sulfide
-
Hyphomicrobium sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
50000
gel filtration
Hyphomicrobium sp.
49000
-
1 * 49000, SDS-PAGE
Hyphomicrobium sp.
Organism
Organism
UniProt
Commentary
Textmining
Hyphomicrobium sp.
-
-
-
Hyphomicrobium sp. EG
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Hyphomicrobium sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
2.13
-
-
Hyphomicrobium sp.
Storage Stability
Storage Stability
Organism
-20C, stable for months
Hyphomicrobium sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
ethyl mercaptan + O2 + H2O
-
395101
Hyphomicrobium sp.
acetaldehyde + H2S + H2O2
-
-
-
?
ethyl mercaptan + O2 + H2O
-
395101
Hyphomicrobium sp. EG
acetaldehyde + H2S + H2O2
-
-
-
?
methanethiol + O2 + H2O
-
395101
Hyphomicrobium sp.
formaldehyde + H2S + H2O2
-
395101
Hyphomicrobium sp.
?
methanethiol + O2 + H2O
-
395101
Hyphomicrobium sp. EG
formaldehyde + H2S + H2O2
-
395101
Hyphomicrobium sp. EG
?
sulfide + O2
-
395101
Hyphomicrobium sp.
sulfur + H2O2
-
395101
Hyphomicrobium sp.
?
sulfide + O2
-
395101
Hyphomicrobium sp. EG
sulfur + H2O2
-
395101
Hyphomicrobium sp. EG
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 49000, SDS-PAGE
Hyphomicrobium sp.
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
50
-
10 min, stable
Hyphomicrobium sp.
65
-
10 min, complete loss of activity
Hyphomicrobium sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.2
-
-
Hyphomicrobium sp.
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.04
-
cyanide
-
Hyphomicrobium sp.
0.042
-
methanethiol
-
Hyphomicrobium sp.
0.09
-
Sulfide
-
Hyphomicrobium sp.
1.4
-
methylamine
-
Hyphomicrobium sp.
4
-
NH4+
-
Hyphomicrobium sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
(NH4)2SO4
-
Hyphomicrobium sp.
cyanide
-
Hyphomicrobium sp.
ethyl mercaptan
-
Hyphomicrobium sp.
methanethiol
above 0.014 mM
Hyphomicrobium sp.
methylamine
-
Hyphomicrobium sp.
NH4+
-
Hyphomicrobium sp.
NH4Cl
-
Hyphomicrobium sp.
Sulfide
non-competitive inhibition of methyl mercaptan oxidation
Hyphomicrobium sp.
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.04
-
cyanide
-
Hyphomicrobium sp.
0.042
-
methanethiol
-
Hyphomicrobium sp.
0.09
-
Sulfide
-
Hyphomicrobium sp.
1.4
-
methylamine
-
Hyphomicrobium sp.
4
-
NH4+
-
Hyphomicrobium sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.005
0.01
methanethiol
-
Hyphomicrobium sp.
0.018
-
ethyl mercaptan
-
Hyphomicrobium sp.
0.06
-
Sulfide
-
Hyphomicrobium sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
50000
gel filtration
Hyphomicrobium sp.
49000
-
1 * 49000, SDS-PAGE
Hyphomicrobium sp.
Purification (Commentary) (protein specific)
Commentary
Organism
-
Hyphomicrobium sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
2.13
-
-
Hyphomicrobium sp.
Storage Stability (protein specific)
Storage Stability
Organism
-20C, stable for months
Hyphomicrobium sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
ethyl mercaptan + O2 + H2O
-
395101
Hyphomicrobium sp.
acetaldehyde + H2S + H2O2
-
-
-
?
ethyl mercaptan + O2 + H2O
-
395101
Hyphomicrobium sp. EG
acetaldehyde + H2S + H2O2
-
-
-
?
methanethiol + O2 + H2O
-
395101
Hyphomicrobium sp.
formaldehyde + H2S + H2O2
-
395101
Hyphomicrobium sp.
?
methanethiol + O2 + H2O
-
395101
Hyphomicrobium sp. EG
formaldehyde + H2S + H2O2
-
395101
Hyphomicrobium sp. EG
?
sulfide + O2
-
395101
Hyphomicrobium sp.
sulfur + H2O2
-
395101
Hyphomicrobium sp.
?
sulfide + O2
-
395101
Hyphomicrobium sp. EG
sulfur + H2O2
-
395101
Hyphomicrobium sp. EG
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 49000, SDS-PAGE
Hyphomicrobium sp.
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
50
-
10 min, stable
Hyphomicrobium sp.
65
-
10 min, complete loss of activity
Hyphomicrobium sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.2
-
-
Hyphomicrobium sp.
Other publictions for EC 1.8.3.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
395104
Kim
-
Isolation and purification of ...
Rhodococcus rhodochrous
Biotechnol. Bioprocess Eng.
5
465-468
2000
-
-
-
-
-
-
3
-
-
-
1
1
-
1
-
-
1
-
-
-
1
-
2
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
1
1
-
-
-
1
-
-
1
-
2
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
395102
Gould
-
Purification and properties of ...
Thiobacillus thioparus, Thiobacillus thioparus TK-m
J. Gen. Microbiol.
138
217-221
1992
-
-
-
-
-
-
7
1
-
-
2
-
-
2
-
-
1
-
-
-
1
-
6
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
1
-
-
2
-
-
-
-
1
-
-
1
-
6
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
395103
Smith
-
Mechanism of oxidation of dime ...
Thiobacillus thioparus, Thiobacillus thioparus E6
J. Gen. Microbiol.
134
3031-3039
1988
-
-
-
-
-
-
1
1
-
-
-
2
-
3
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
1
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
395101
Suylen
-
Methyl mercaptan oxidase, a ke ...
Hyphomicrobium sp., Hyphomicrobium sp. EG
J. Gen. Microbiol.
133
2989-2997
1987
-
-
-
-
-
-
8
3
-
-
2
-
-
2
-
-
1
-
-
-
1
1
6
1
-
-
-
2
-
1
-
-
-
5
-
-
-
-
-
-
-
-
-
-
8
5
3
-
-
2
-
-
-
-
1
-
-
1
1
6
1
-
-
2
-
1
-
-
-
-
-
-
-
-
-