Literature summary for 1.8.3.2 extracted from

Faccio, G.; Kruus, K.; Buchert, J.; Saloheimo, M.
Production and characterisation of AoSOX2 from Aspergillus oryzae, a novel flavin-dependent sulfhydryl oxidase with good pH and temperature stability (2011), Appl. Microbiol. Biotechnol., 90, 941-949.

Search for more literature for 1.8.3.2

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Trichoderma reesei	Aspergillus oryzae

Inhibitors

Inhibitors	Comment	Organism	Structure
MgSO4	1 mM, 55% residual activity	Aspergillus oryzae
Na2SO4	1 mM, 66% residual activity	Aspergillus oryzae
sodium dodecylsulfate	1 mM, 57% residual activity	Aspergillus oryzae
ZnSO4	1 mM, 95% loss of activity	Aspergillus oryzae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.9	-	L-Cys	pH 7.5, 20°C	Aspergillus oryzae
3.7	-	glutathione	pH 7.5, 20°C	Aspergillus oryzae
4.9	-	dithiothreitol	pH 7.5, 20°C	Aspergillus oryzae
7.9	-	2-mercaptoethanol	pH 7.5, 20°C	Aspergillus oryzae
13.1	-	D-Cys	pH 7.5, 20°C	Aspergillus oryzae

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	1 mM, 95% loss of activity	Aspergillus oryzae

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
42412	-	2 * 42412, MALDI-TOF, mature protein, 2 * 45000, SDS-PAGE	Aspergillus oryzae
45000	-	2 * 42412, MALDI-TOF, mature protein, 2 * 45000, SDS-PAGE	Aspergillus oryzae
78000	-	gel filtration	Aspergillus oryzae

Organism

Organism	UniProt	Comment	Textmining
Aspergillus oryzae	Q2U4P3	-	-
Aspergillus oryzae ATCC 42149	Q2U4P3	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
proteolytic modification	sequence contains a signal peptide of 23 amino acids	Aspergillus oryzae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2 2-mercaptoethanol + O2	-	Aspergillus oryzae	(ethyldisulfanyl)ethane + H2O2	-	?
2 2-mercaptoethanol + O2	-	Aspergillus oryzae ATCC 42149	(ethyldisulfanyl)ethane + H2O2	-	?
2 D-Cys + O2	-	Aspergillus oryzae	D-cystine + H2O2	-	?
2 D-Cys + O2	-	Aspergillus oryzae ATCC 42149	D-cystine + H2O2	-	?
2 dithiothreitol + O2	-	Aspergillus oryzae	dithiothreitol disulfide + H2O2	-	?
2 dithiothreitol + O2	-	Aspergillus oryzae ATCC 42149	dithiothreitol disulfide + H2O2	-	?
2 glutathione + O2	-	Aspergillus oryzae	glutathione disulfide + H2O2	-	?
2 glutathione + O2	-	Aspergillus oryzae ATCC 42149	glutathione disulfide + H2O2	-	?
2 L-Cys + O2	-	Aspergillus oryzae	L-cystine + H2O2	-	?
2 L-Cys + O2	-	Aspergillus oryzae ATCC 42149	L-cystine + H2O2	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 42412, MALDI-TOF, mature protein, 2 * 45000, SDS-PAGE	Aspergillus oryzae

Synonyms

Synonyms	Comment	Organism
Sox2	-	Aspergillus oryzae

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
50	-	24 h, 42% residual activity	Aspergillus oryzae
60	-	1 h, 68% residual activity	Aspergillus oryzae
75	-	melting temperature	Aspergillus oryzae

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
6800	-	L-Cys	pH 7.5, 20°C	Aspergillus oryzae
55400	-	2-mercaptoethanol	pH 7.5, 20°C	Aspergillus oryzae
78600	-	D-Cys	pH 7.5, 20°C	Aspergillus oryzae
97500	-	glutathione	pH 7.5, 20°C	Aspergillus oryzae
337800	-	dithiothreitol	pH 7.5, 20°C	Aspergillus oryzae

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	-	Aspergillus oryzae

pH Range

pH Minimum	pH Maximum	Comment	Organism
5	8.5	more than 80% of maximum activity	Aspergillus oryzae

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Aspergillus oryzae

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
5980	-	D-Cys	pH 7.5, 20°C	Aspergillus oryzae
7000	-	2-mercaptoethanol	pH 7.5, 20°C	Aspergillus oryzae
7600	-	L-Cys	pH 7.5, 20°C	Aspergillus oryzae
26500	-	glutathione	pH 7.5, 20°C	Aspergillus oryzae
69230	-	dithiothreitol	pH 7.5, 20°C	Aspergillus oryzae

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Release 2023.1 (January 2023)