Cloned (Comment) | Organism |
---|---|
gene cpQSOx1, primary gene sequence, DNA and amino acid sequence analysis, stable expression of the wild-type enzyme in MCF-7 cells with intra- and extracellular localization of the recombinant enzyme, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) | Cavia porcellus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | enzyme is secreted | Cavia porcellus | - |
- |
perikaryon | - |
Cavia porcellus | 43204 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68000 | - |
x * 68000, recombinant His-tagged enzyme, SDS-PAGE | Cavia porcellus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
R-SH + O2 | Cavia porcellus | enzyme plays a role in synaptic strengthening and in redox activities in the brain | R-S-S-R + H2O2 | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cavia porcellus | O08841 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel chelate chromatography | Cavia porcellus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
basal forebrain | - |
Cavia porcellus | - |
brain | detailed tissue distribution analysis in brain, overview | Cavia porcellus | - |
brain stem | - |
Cavia porcellus | - |
central nervous system | highest enzyme expression in reticular structures, such as the basal forebrain, reticular thalamic nucleus, and reticular nuclei of the brainstem | Cavia porcellus | - |
endometrium | specific expression in glandular and luminal epithelial cells | Cavia porcellus | - |
epithelium | secretory, endometrial | Cavia porcellus | - |
lung | - |
Cavia porcellus | - |
neuron | - |
Cavia porcellus | - |
ovary | - |
Cavia porcellus | - |
secretory cell | - |
Cavia porcellus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dithiothreitol + O2 | - |
Cavia porcellus | dithiothreitol disulfide + H2O2 | - |
ir | |
R-SH + O2 | - |
Cavia porcellus | R-S-S-R + H2O2 | - |
ir | |
R-SH + O2 | enzyme plays a role in synaptic strengthening and in redox activities in the brain | Cavia porcellus | R-S-S-R + H2O2 | - |
ir |
Subunits | Comment | Organism |
---|---|---|
? | x * 68000, recombinant His-tagged enzyme, SDS-PAGE | Cavia porcellus |
Synonyms | Comment | Organism |
---|---|---|
More | enzyme belongs to the sulfhydryl oxidase/Quiescin Q6 family | Cavia porcellus |
QSOx1 | - |
Cavia porcellus |
sulfhydryl oxidase | - |
Cavia porcellus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Cavia porcellus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Cavia porcellus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | dependent on | Cavia porcellus |