Protein Variants | Comment | Organism |
---|---|---|
D342K | significant decrease in the intramolecular electron transfer rate constant, kcat value is higher than the corresponding intramolecular electron transfer rate constant values, and the redox potentials of both metal centers are affected | Homo sapiens |
R472D | significant decrease in the intramolecular electron transfer rate constant, and the redox potentials of both metal centers are affected | Homo sapiens |
R472D/D342K | mutation reverses the charges of the salt bridge components, large decrease in intramolecular electron transfer rate constant | Homo sapiens |
R472K | 40% increase in catalytic efficiency | Homo sapiens |
R472M | significant decrease in the intramolecular electron transfer rate constant, kcat value is higher than the corresponding intramolecular electron transfer rate constant values, and the redox potentials of both metal centers are affected | Homo sapiens |
R472Q | significant decrease in the intramolecular electron transfer rate constant, kcat value is higher than the corresponding intramolecular electron transfer rate constant values, and the redox potentials of both metal centers are affected | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0032 | - |
sulfite | mutant R472K, pH 7.6, 25°C | Homo sapiens | |
0.011 | - |
sulfite | wild-type, pH 8.0, 25°C | Homo sapiens | |
0.012 | - |
sulfite | mutant D342K, pH 7.6, 25°C | Homo sapiens | |
0.022 | - |
sulfite | mutant R472Q, pH 7.6, 25°C | Homo sapiens | |
0.023 | - |
sulfite | mutant R472D, pH 7.6, 25°C | Homo sapiens | |
0.023 | - |
sulfite | mutant R472D/D342K, pH 7.6, 25°C | Homo sapiens | |
0.042 | - |
sulfite | mutant R472M, pH 7.6, 25°C | Homo sapiens | |
0.051 | - |
sulfite | mutant R472D, pH 6.5, 25°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sulfite + O2 + H2O | - |
Homo sapiens | sulfate + H2O2 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.7 | - |
sulfite | mutant R472D, pH 6.5, 25°C | Homo sapiens | |
14.1 | - |
sulfite | mutant R472D, pH 7.6, 25°C | Homo sapiens | |
18.5 | - |
sulfite | mutant R472K, pH 7.6, 25°C | Homo sapiens | |
23 | - |
sulfite | mutant D342K, pH 7.6, 25°C | Homo sapiens | |
26.6 | - |
sulfite | mutant R472Q, pH 7.6, 25°C | Homo sapiens | |
26.9 | - |
sulfite | wild-type, pH 8.0, 25°C | Homo sapiens | |
27 | - |
sulfite | mutant R472M, pH 7.6, 25°C | Homo sapiens | |
47 | - |
sulfite | mutant R472D/D342K, pH 7.6, 25°C | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11 | - |
sulfite | mutant R472D, pH 6.5, 25°C | Homo sapiens | |
610 | - |
sulfite | mutant R472D, pH 7.6, 25°C | Homo sapiens | |
640 | - |
sulfite | mutant R472M, pH 7.6, 25°C | Homo sapiens | |
1200 | - |
sulfite | mutant R472Q, pH 7.6, 25°C | Homo sapiens | |
1900 | - |
sulfite | mutant D342K, pH 7.6, 25°C | Homo sapiens | |
2000 | - |
sulfite | mutant R472D/D342K, pH 7.6, 25°C | Homo sapiens | |
2400 | - |
sulfite | wild-type, pH 8.0, 25°C | Homo sapiens | |
5800 | - |
sulfite | mutant R472K, pH 7.6, 25°C | Homo sapiens |