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Literature summary for 1.8.3.1 extracted from

  • Johnson-Winters, K.; Davis, A.C.; Arnold, A.R.; Berry, R.E.; Tollin, G.; Enemark, J.H.
    Probing the role of a conserved salt bridge in the intramolecular electron transfer kinetics of human sulfite oxidase (2013), J. Biol. Inorg. Chem., 18, 645-653.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
D342K significant decrease in the intramolecular electron transfer rate constant, kcat value is higher than the corresponding intramolecular electron transfer rate constant values, and the redox potentials of both metal centers are affected Homo sapiens
R472D significant decrease in the intramolecular electron transfer rate constant, and the redox potentials of both metal centers are affected Homo sapiens
R472D/D342K mutation reverses the charges of the salt bridge components, large decrease in intramolecular electron transfer rate constant Homo sapiens
R472K 40% increase in catalytic efficiency Homo sapiens
R472M significant decrease in the intramolecular electron transfer rate constant, kcat value is higher than the corresponding intramolecular electron transfer rate constant values, and the redox potentials of both metal centers are affected Homo sapiens
R472Q significant decrease in the intramolecular electron transfer rate constant, kcat value is higher than the corresponding intramolecular electron transfer rate constant values, and the redox potentials of both metal centers are affected Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0032
-
sulfite mutant R472K, pH 7.6, 25°C Homo sapiens
0.011
-
sulfite wild-type, pH 8.0, 25°C Homo sapiens
0.012
-
sulfite mutant D342K, pH 7.6, 25°C Homo sapiens
0.022
-
sulfite mutant R472Q, pH 7.6, 25°C Homo sapiens
0.023
-
sulfite mutant R472D, pH 7.6, 25°C Homo sapiens
0.023
-
sulfite mutant R472D/D342K, pH 7.6, 25°C Homo sapiens
0.042
-
sulfite mutant R472M, pH 7.6, 25°C Homo sapiens
0.051
-
sulfite mutant R472D, pH 6.5, 25°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sulfite + O2 + H2O
-
Homo sapiens sulfate + H2O2
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.7
-
sulfite mutant R472D, pH 6.5, 25°C Homo sapiens
14.1
-
sulfite mutant R472D, pH 7.6, 25°C Homo sapiens
18.5
-
sulfite mutant R472K, pH 7.6, 25°C Homo sapiens
23
-
sulfite mutant D342K, pH 7.6, 25°C Homo sapiens
26.6
-
sulfite mutant R472Q, pH 7.6, 25°C Homo sapiens
26.9
-
sulfite wild-type, pH 8.0, 25°C Homo sapiens
27
-
sulfite mutant R472M, pH 7.6, 25°C Homo sapiens
47
-
sulfite mutant R472D/D342K, pH 7.6, 25°C Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
11
-
sulfite mutant R472D, pH 6.5, 25°C Homo sapiens
610
-
sulfite mutant R472D, pH 7.6, 25°C Homo sapiens
640
-
sulfite mutant R472M, pH 7.6, 25°C Homo sapiens
1200
-
sulfite mutant R472Q, pH 7.6, 25°C Homo sapiens
1900
-
sulfite mutant D342K, pH 7.6, 25°C Homo sapiens
2000
-
sulfite mutant R472D/D342K, pH 7.6, 25°C Homo sapiens
2400
-
sulfite wild-type, pH 8.0, 25°C Homo sapiens
5800
-
sulfite mutant R472K, pH 7.6, 25°C Homo sapiens