Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Sinorhizobium meliloti |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Sinorhizobium meliloti | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Molybdenum | recombinant enzyme contains 0.69 molecules of Mo per protein subunit | Sinorhizobium meliloti |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42669 | - |
2 * 42669, mass spectrometry, 2 * 42791, calculated | Sinorhizobium meliloti |
42791 | - |
2 * 42669, mass spectrometry, 2 * 42791, calculated | Sinorhizobium meliloti |
71000 | - |
gel filtration | Sinorhizobium meliloti |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sinorhizobium meliloti | F7X8Y3 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sulfite + cytochrome c | substrates horse heart cytochrome c, and recombinant Starkeya novella cytochrome c are only reduced to about 40% while Sinorhizobium meliloti cytochrome c is almost completely reduced. Enzyme interacts with two small redox proteins, a cytochrome c and a Cu containing pseudoazurin, that are encoded in the same operon and are co-transcribed with the sorT gene | Sinorhizobium meliloti | sulfate + reduced cytochrome c | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 42669, mass spectrometry, 2 * 42791, calculated | Sinorhizobium meliloti |
Synonyms | Comment | Organism |
---|---|---|
SorT | - |
Sinorhizobium meliloti |
General Information | Comment | Organism |
---|---|---|
physiological function | enzyme is able to couple efficiently to a cytochrome c isolated from the same organism despite being unable to efficiently reduce horse heart cytochrome c. Enzyme interacts with two small redox proteins, a cytochrome c and a Cu containing pseudoazurin, that are encoded in the same operon and are co-transcribed with the sorT gene. The pseudoazurin may act as an intermediate electron shuttle between. The protein system appears to couple directly to the respiratory chain, most likely to a cytochrome oxidase | Sinorhizobium meliloti |